Solubilization of IgA immune precipitates by complement.

G W Miller

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PubMed · 977953

Solubilization of IgA immune precipitates by complement.

G W Miller

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Abstract

The complement (C) system can solubilize immune precipitates prepared with antibodies of the IgG class, and apparently also of the IgM class. This paper shows that C can also solubilize immune precipitates prepared with two different antigen-binding IgA myeloma proteins. Immune precipitates were prepared with a) mouse myeloma protein TEPC 15 and PC-KLH, and b) mouse myeloma protein MOPC 315 and DNP-BSA. The precipitates could be solubilized by fresh mouse serum, but not by zymosan- or heat-inactivated serum. The rate of solubilization was not affected by the removal of of Ca++ ions. These results verify that the C-mediated solubilization effect can proceed entirely via the alternative C pathway, and lend increased support to the view that the effect is a general phenomenon, not restricted to a particular antibody class or type of antigen.

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Animals Antigen-Antibody Complex Calcium Cations, Divalent Complement System Proteins Hemocyanins Immune Sera Immunoglobulin A Magnesium Mice Mice, Inbred BALB C Myeloma Proteins Nitrobenzenes Serum Albumin, Bovine Solubility Journal Article Research Support, U.S. Gov't, P.H.S.

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G W Miller. 1976. Solubilization of IgA immune precipitates by complement.. https://pubmed.ncbi.nlm.nih.gov/977953/

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