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PubMed · 9783163

Thermozymes: biotechnology and structure-function relationships.

J G Zeikus·C Vieille·A Savchenko
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Abstract

Recent findings on the biochemical and molecular features of the following thermozymes are presented, based on their biotechnological use: alpha-amylase and amylopullulanase, used in starch processing; glucose isomerase, used in sweetener production; alcohol dehydrogenase, used in chemical synthesis; and alkaline phosphatase, used in diagnostics. The corresponding genes and recombinant proteins have been characterized in terms of sequence similarities, specific activities, thermophilicity, and unfolding kinetics. Site-directed and nested deletion mutagenesis were used to understand structure-function relationships. All these thermozymes display higher stability and activity than their counterparts currently used in the biotechnology industry.

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Alcohol DehydrogenaseAldose-Ketose IsomerasesAlkaline PhosphataseBacteriaBacterial ProteinsBiotechnologyEnzyme StabilityEnzymesGlycoside HydrolasesHot TemperatureModels, Molecularalpha-AmylasesComparative StudyJournal ArticleReview

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J G Zeikus, C Vieille, A Savchenko. 1998. Thermozymes: biotechnology and structure-function relationships.. https://doi.org/10.1007/s007920050058

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