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PubMed · 9792497

Recycling immunoaffinity chromatography for multiple analyte analysis in biological samples.

T M Phillips·J M Krum
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Abstract

The ability to isolate and measure multiple complex analytes in a single biological sample holds great potential in many biomedical fields, especially immunology and diagnostic clinical chemistry. We have developed a procedure involving recycling immunoaffinity chromatography for the simultaneous measurement of a number of analytes in a single sample. The procedure is based on the passage of a fluorochrome-labelled sample through a battery of small immunoaffinity columns, each column extracting a single analyte. Detection is achieved by acid elution of the bound analytes and laser-induced fluorescence. We have applied this system to a number of different biological fluids and found that it is capable of reliably isolating and measuring up to ten different cytokines in a 25-microl sample of human body fluid.

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AntibodiesChromatography, AffinityCytokinesHumansLasersReproducibility of ResultsJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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T M Phillips, J M Krum. 1998-09-11. Recycling immunoaffinity chromatography for multiple analyte analysis in biological samples.. https://doi.org/10.1016/s0378-4347(97)00675-0

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