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PubMed · 9795190

cDNA sequence analysis, chromosomal assignment and expression pattern of the gene coding for integral membrane protein 2B.

K Pittois·W Deleersnijder·J Merregaert
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Abstract

The complete cDNA of the mouse integral membrane protein 2B gene (Itm2b) was determined by sequence analysis of expressed sequence tag (EST) clone L26775 and a clone isolated from a cDNA library of the osteogenic stromal cell line MN7 (Mathieu et al., 1992. Calcif. Tissue Int. 50, 362-371) and by 5' rapid amplification of cDNA ends (RACE). Alignment of different mouse ESTs confirmed the entire sequence. Northern blot analysis of different neonatal and adult mouse tissues showed that Itm2b is ubiquitously expressed. There are three mRNAs with different lengths in neonatal as well as in adult tissues, originating from alternative polyadenylation by usage of one consensus and two additional variant polyadenylation signals. The cDNA sequence of the human Itm2b homolog (ITM2B) was assembled using data from available human ESTs. Both the mouse and the human gene code for a protein of 266 amino acids (aa) that is homologous to a previously described integral membrane protein, Itm2A, of which the expression is restricted to osteo- and chondrogenic tissues. Itm2A and Itm2B belong to a family of type II integral membrane proteins, which contains a third member, Itm2C (Deleersnijder et al., 1996. J. Biol. Chem. 271, 19475-19482). The human ITM2B and mouse Itm2b genes were previously mapped as unknown ESTs to conserved syntenic regions Homo sapiens 13q12-13 and Mus musculus 14.

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Adaptor Proteins, Signal TransducingAlgorithmsAmino Acid SequenceAmyloidAnimalsBase SequenceBone and BonesCell LineChromosome MappingConsensus SequenceDNA PrimersDNA, ComplementaryDatabases, FactualExpressed Sequence TagsGene Expression RegulationHumansMembrane GlycoproteinsMembrane ProteinsMiceMolecular Sequence DataOrgan SpecificityRandom Amplified Polymorphic DNA TechniqueReverse Transcriptase Polymerase Chain ReactionStromal CellsTranscription, GeneticJournal ArticleResearch Support, Non-U.S. Gov't

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K Pittois, W Deleersnijder, J Merregaert. 1998-09-14. cDNA sequence analysis, chromosomal assignment and expression pattern of the gene coding for integral membrane protein 2B.. https://doi.org/10.1016/s0378-1119(98)00354-0

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