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PubMed · 9797329

A novel screening method for isolating exopolysaccharide-deficient mutants.

M Liu·J E González·L B Willis·G C Walker
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Abstract

A screening method based on differential staining of the wild type and exopolysaccharide-deficient mutants of Rhizobium (Sinorhizobium) meliloti by the lipophilic dye Sudan Black B is described. Mutants defective in the production of either succinoglycan or EPS II (galactoglucan) were isolated by using this method, which might also prove useful for isolating exopolysaccharide-defective derivatives of other bacteria.

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Azo CompoundsBacteriological TechniquesColoring AgentsGenetic TechniquesNaphthalenesPolysaccharides, BacterialSinorhizobium melilotiJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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M Liu, J E González, L B Willis, G C Walker. 1998. A novel screening method for isolating exopolysaccharide-deficient mutants.. https://doi.org/10.1128/aem.64.11.4600-4602.1998

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