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DNA typing for BoLA class I using sequence-specific primers (PCR-SSP).

S A Ellis·K A Staines·M J Stear·E J Hensen·W I Morrison
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Abstract

The analysis of cattle MHC (BoLA) class I gene expression is an essential component of studies on immune responses and susceptibility to disease. International BoLA workshops have generated data and reagents that allow discrimination of class I molecules at the haplotype level, but progress has been limited by difficulties encountered in defining single alleles. Our aim in this study was to develop a DNA-based system for improved identification of expressed class I alleles, utilizing available cDNA sequences derived from cattle carrying a series of serologically defined class I specificities. This method has allowed more accurate typing of animals for expression of the class I genes present within a small number of haplotypes. The method has also reliably differentiated between allelic variants (identified by prior sequence analysis) and has split existing serological specificities. The data show that MHC class I genes in cattle are more polymorphic than demonstrated by serology and biochemical analysis.

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AllelesAnimalsCattleDNADNA PrimersHistocompatibility Antigens Class IPolymerase Chain ReactionJournal ArticleResearch Support, Non-U.S. Gov't

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S A Ellis, K A Staines, M J Stear, E J Hensen, W I Morrison. 1998. DNA typing for BoLA class I using sequence-specific primers (PCR-SSP).. https://doi.org/10.1046/j.1365-2370.1998.00112.x

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