Thyroid stimulating hormone and selenium supply interact to regulate selenoenzyme gene expression in thyroid cells (FRTL-5) in culture.

S Villette; G Bermano; J R Arthur; J E Hesketh

doi:10.1016/s0014-5793(98)01280-0

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Thyroid stimulating hormone and selenium supply interact to regulate selenoenzyme gene expression in thyroid cells (FRTL-5) in culture.

S Villette·G Bermano·J R Arthur·J E Hesketh

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Abstract

In the absence of a sodium selenite supplement, FRTL-5 cells showed a reduced activity of cytosolic glutathione peroxidase (cGSH-Px), a marker of selenium status, indicating the cells were Se-deficient. Se-deficient cells showed a 65% reduction in cGSH-Px mRNA abundance but little change in abundance of either phospholipid hydroperoxide glutathione peroxidase or type 1 deiodinase (IDI) mRNA. In Se-replete cells increased thyroid stimulating hormone (TSH) caused a small decrease in IDI abundance but in Se-deficient cells TSH caused a large increase. The results indicate an interaction between TSH and Se status in the regulation of thyroid selenoenzyme synthesis.

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Animals Blotting, Northern Cell Line Culture Media Cytosol Epithelial Cells Gene Expression Regulation, Enzymologic Glutathione Peroxidase Humans Iodide Peroxidase Phospholipid Hydroperoxide Glutathione Peroxidase RNA, Messenger Rats Selenium Thyroid Gland Thyrotropin Journal Article Research Support, Non-U.S. Gov't

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S Villette, G Bermano, J R Arthur, J E Hesketh. 1998-10-30. Thyroid stimulating hormone and selenium supply interact to regulate selenoenzyme gene expression in thyroid cells (FRTL-5) in culture.. https://doi.org/10.1016/s0014-5793(98)01280-0

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