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Generation, identification, and evaluation of expressed sequence tags from different developmental stages of the Asian blood fluke Schistosoma japonicum.

J Fan·D J Minchella·S R Day·D P McManus·W U Tiu·P J Brindley
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Abstract

Here we report 658 expressed sequence tags (ESTs) generated from the 5'-termini of clones randomly selected from directional cDNA libraries constructed from mRNAs from three developmental stages of Schistosoma japonicum. Putative identifications were assigned to 46. 2% of the ESTs; 6.4% were previously known from S. japonicum, 5.6% were previously known from S. mansoni, 34.2% were known from other organisms, and the remaining 53.8% may represent S. japonicum-specific genes. These 658 ESTs appeared to be derived from 457 unique genes, which together represent 2 to 3% of the 15,000 to 20,000 genes predicted to occur in the schistosome genome.

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AnimalsBase SequenceDNA PrimersDNA, ComplementaryDNA, HelminthExpressed Sequence TagsFemaleGene Expression Regulation, DevelopmentalGene LibraryGenes, HelminthMaleMiceRNA, HelminthRNA, MessengerRabbitsSchistosoma japonicumJournal ArticleResearch Support, Non-U.S. Gov't

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J Fan, D J Minchella, S R Day, D P McManus, W U Tiu, P J Brindley. 1998-11-18. Generation, identification, and evaluation of expressed sequence tags from different developmental stages of the Asian blood fluke Schistosoma japonicum.. https://doi.org/10.1006/bbrc.1998.9491

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