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A DNA polymerase stop assay for G-quadruplex-interactive compounds.

H Han·L H Hurley·M Salazar
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Abstract

We have developed and characterized an assay for G-quadruplex-interactive compounds that makes use of the fact that G-rich DNA templates present obstacles to DNA synthesis by DNA polymerases. Using Taq DNA polymerase and the G-quadruplex binding 2, 6-diamidoanthraquinone BSU-1051, we find that BSU-1051 leads to enhanced arrest of DNA synthesis in the presence of K+by stabilizing an intramolecular G-quadruplex structure formed by four repeats of either TTGGGG or TTAGGG in the template strand. The data provide additional evidence that BSU-1051 modulates telomerase activity by stabilization of telomeric G-quadruplex DNA and point to a polymerase arrest assay as a sensitive method for screening for G-quadruplex-interactive agents with potential clinical utility.

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AnthraquinonesCations, MonovalentDNAG-QuadruplexesGuanine NucleotidesNucleic Acid ConformationPotassiumTaq PolymeraseTelomeraseJournal ArticleResearch Support, U.S. Gov't, P.H.S.

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H Han, L H Hurley, M Salazar. 1999-01-15. A DNA polymerase stop assay for G-quadruplex-interactive compounds.. https://doi.org/10.1093/nar%2F27.2.537

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