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PubMed · 9868915

Multiple sample PCR amplification and electrophoretic analysis on a microchip.

L C Waters·S C Jacobson·N Kroutchinina·J Khandurina·R S Foote·J M Ramsey
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Abstract

Polymerase chain reactions (PCRs) were carried out on as many as four DNA samples at a time on a microchip device. The PCR products were then analyzed, either individually or together on the same device, by microchip gel electrophoresis. A standard PCR protocol was used to amplify 199- and 500-base pair (bp) regions of bacteriophage lambda DNA and 346- and 410-bp regions of E. coli genomic and plasmid DNAs, respectively. Thermal lysis of the bacteria was integrated into the PCR cycle. A product sizing medium, poly(dimethylacrylamide), and an intercalating dye for fluorescence detection were used in the electrophoretic analysis of the products. PCR product sizes were determined by coelectrophoresis with marker DNA.

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DNA, BacterialDNA, ViralElectrophoresisGene AmplificationPolymerase Chain ReactionJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.

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L C Waters, S C Jacobson, N Kroutchinina, J Khandurina, R S Foote, J M Ramsey. 1998-12-15. Multiple sample PCR amplification and electrophoretic analysis on a microchip.. https://doi.org/10.1021/ac980447e

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