Hydrolase-catalyzed beta-mannosylations.

N Taubken; J Thiem

doi:10.1023/a:1006999614526

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PubMed · 9870351

Hydrolase-catalyzed beta-mannosylations.

N Taubken·J Thiem

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Abstract

Transmannosylations catalysed by beta-mannosidase from snail viscera or beta-galactosidase from Aspergillus oryzae were accomplished with 4-nitrophenyl beta-D-mannopyranoside as donor substrate. With suitable hydrophobic acceptor molecules preferentially beta1-4-linked disaccharides were obtained. The activities of both glycosidases in buffer cosolvent mixtures were determined, and conditions for their immobilization were elaborated and optimized. A model of the enzymic transfer mechanism is suggested.

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Animals Aspergillus oryzae Disaccharides Enzymes, Immobilized Glycoside Hydrolases Glycosides Glycosylation Hydrogen-Ion Concentration Mannosidases Mannosides Models, Chemical Nuclear Magnetic Resonance, Biomolecular Snails Viscera beta-Galactosidase beta-Mannosidase Journal Article Research Support, Non-U.S. Gov't

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N Taubken, J Thiem. 1998. Hydrolase-catalyzed beta-mannosylations.. https://doi.org/10.1023/a%3A1006999614526

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