Time-resolved fluoroimmunoassay for plasma enterolactone.

H Adlercreutz; G J Wang; O Lapcík; R Hampl; K Wähälä; T Mäkelä; K Lusa; M Talme; H Mikola

doi:10.1006/abio.1998.2886

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Time-resolved fluoroimmunoassay for plasma enterolactone.

H Adlercreutz·G J Wang·O Lapcík·R Hampl·K Wähälä·T Mäkelä·K Lusa·M Talme·H Mikola

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Abstract

We present a method for the determination of the lignan enterolactone in plasma (serum). This compound, produced by intestinal bacteria from matairesinol and secoisolariciresinol in fiber-rich food, is a biomarker related to the intake of a healthy diet. The method is based on time-resolved fluoroimmunoassay using a europium chelate as a label. After synthesis of 5'-O-carboxymethoxyenterolactone the compound is coupled to bovine serum albumin and then used as antigen in immunization of rabbits. The tracer with the europium chelate is synthesized using the same 5'-derivative of enterolactone. After enzymatic hydrolysis and ether extraction the immunoassay is carried out using the VICTOR 1420 multilabel counter (Wallac Oy, Turku, Finland). No antiserum cross-reactivity with available lignans, isoflavonoids, or flavonoids could be detected. The intraassay and interassay coefficients of variation at different concentrations vary 4.6-6.0 and 5.5-9.9, respectively. The working range of the assay is 1.5-540 nmol/liter. We measured enterolactone in serum/plasma of 224 Finnish subjects: 98.8% of the subjects had values <100 nmol/liter, 38.0% had 20-39.9 nmol/liter, and 34.4% had <20 nmol/liter.

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4-Butyrolactone Fluoroimmunoassay Gas Chromatography-Mass Spectrometry Humans Lignans Sensitivity and Specificity Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

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H Adlercreutz, G J Wang, O Lapcík, R Hampl, K Wähälä, T Mäkelä, K Lusa, M Talme, H Mikola. 1998-12-15. Time-resolved fluoroimmunoassay for plasma enterolactone.. https://doi.org/10.1006/abio.1998.2886

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