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New cloning vectors with temperature-sensitive replication.

G J Phillips
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Abstract

A series of cloning vectors with conditional, temperature-sensitive replication that are selectable with ampicillin, chloramphenicol, and kanamycin has been constructed. These vectors are derivatives of a pSC101 mutant that can replicate only at low temperatures. The cloning vectors carry a number of unique restriction sites and provide for screening of recombinant plasmids by alpha complementation. These vectors have proven useful for a variety of applications where conditional replication of a recombinant plasmid is desired.

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Cloning, MolecularDNA ReplicationEscherichia coliGenetic Complementation TestGenetic VectorsPlasmidsRestriction MappingTemperatureJournal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, Non-P.H.S.Research Support, U.S. Gov't, P.H.S.

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BibTeXRIS

G J Phillips. 1999. New cloning vectors with temperature-sensitive replication.. https://doi.org/10.1006/plas.1998.1380

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