Intracellular detection assays for high-throughput screening.

J E González; P A Negulescu

doi:10.1016/s0958-1669(98)80141-9

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Intracellular detection assays for high-throughput screening.

J E González·P A Negulescu

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Abstract

New optical assay methods promise to accelerate the use of living cells in screens for drug discovery. Most of these methods employ either fluorescent or luminescent read-outs and allow cell-based assays for most targets, including receptors, ion channels and intracellular enzymes. Furthermore, genetically encoded probes offer the possibility of custom-engineered biosensors for intracellular biochemistry, specifically localized targets, and protein-protein interactions.

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Animals Cells, Cultured Drug Evaluation, Preclinical Enzymes Genes, Reporter Green Fluorescent Proteins Humans Ion Channels Luminescent Measurements Luminescent Proteins Receptors, Cell Surface Recombinant Proteins Spectrometry, Fluorescence Transfection Journal Article Review

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J E González, P A Negulescu. 1998. Intracellular detection assays for high-throughput screening.. https://doi.org/10.1016/s0958-1669(98)80141-9

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