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PubMed · 990188

Globin synthesis in normal human bone marrow.

G Chalevelakis·J B Clegg·D J Weatherall
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Abstract

Globin synthesis has been studied by in vitro labelling with radioactive amino acids in 60 normal human bone-marrow samples. Under the conditions routinely used to fractionate alpha and beta chains by chromatography alpha/beta production ratios ranging from 0.5 to 1.0 were obtained, depending on the method of sample treatment. This variation was due entirely to the presence of non-haem proteins derived from white cells which chromagraphy with globin on CM-cellulose. Purification of globin on Sephadex G100 and fractionation of alpha and beta globin chains by a modified chromatographic system resulted in alpha/beta ratios of unity. The relevance of these findings to the study of marrows in which there is unbalanced globin chain production is discussed.

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Blood ProteinsBone MarrowCentrifugationChromatography, DEAE-CelluloseChromatography, GelDextransErythrocyte MembraneGlobinsHumansIsoleucineLeukocytesLysineTime FactorsTritiumJournal Article

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BibTeXRIS

G Chalevelakis, J B Clegg, D J Weatherall. 1976. Globin synthesis in normal human bone marrow.. https://doi.org/10.1111/j.1365-2141.1976.tb03600.x

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