Expression profiling using cDNA microarrays.

D J Duggan; M Bittner; Y Chen; P Meltzer; J M Trent

doi:10.1038/4434

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PubMed · 9915494

Expression profiling using cDNA microarrays.

D J Duggan·M Bittner·Y Chen·P Meltzer·J M Trent

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Abstract

cDNA microarrays are capable of profiling gene expression patterns of tens of thousands of genes in a single experiment. DNA targets, in the form of 3' expressed sequence tags (ESTs), are arrayed onto glass slides (or membranes) and probed with fluorescent- or radioactively-labelled cDNAs. Here, we review technical aspects of cDNA microarrays, including the general principles, fabrication of the arrays, target labelling, image analysis and data extraction, management and mining.

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Animals DNA, Complementary Databases, Factual Expressed Sequence Tags Gene Expression Humans Image Processing, Computer-Assisted Molecular Probe Techniques Oligonucleotide Array Sequence Analysis Journal Article Review

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D J Duggan, M Bittner, Y Chen, P Meltzer, J M Trent. 1999. Expression profiling using cDNA microarrays.. https://doi.org/10.1038/4434

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