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Protein identification using 20-minute Edman cycles and sequence mixture analysis.

W J Henzel·J Tropea·D Dupont
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Abstract

We have developed a 20-min Edman cycle and a multiple sample horizontal flow reactor for the sequence analysis of PVDF-electroblotted proteins. The 20-min cycle uses a 12-min C18 phenylthiohydantoin separation. This cycle and separation is compatible with most Applied Biosystems sequencers. Using this rapid cycle, 10 residues on six different proteins can be completed within a 24-h period. We also demonstrate the use of an algorithm that can sort mixture sequences derived from PVDF bands that contain coeluting proteins.

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AlgorithmsAmino Acid SequenceHumansMembranes, ArtificialMolecular Sequence DataPeptide MappingPhenylthiohydantoinPolyvinylsProteinsRecombinant ProteinsFluorocarbon PolymersJournal Article

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BibTeXRIS

W J Henzel, J Tropea, D Dupont. 1999-02-01. Protein identification using 20-minute Edman cycles and sequence mixture analysis.. https://doi.org/10.1006/abio.1998.2981

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