Protein S-nitrosating agents.

Z Guo; N Miranda; P G Wang

doi:10.1016/s0076-6879(99)01089-7

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PubMed · 9919574

Protein S-nitrosating agents.

Z Guo·N Miranda·P G Wang

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Abstract

A series of peptidyl N-nitrosoanilines were designed, synthesized, and evaluated as inactivators of cysteine protease papain. These new compounds exhibited different inhibitory activities toward cysteine protease papain in a time- and concentration-dependent manner with second-order rate constants (ki/KI) ranging over two orders of magnitude from 0.604 M-1 sec-1 (1) to 100.36 M-1 sec-1 (7). Formation of the S-NO bond in papain is corroborated by evidence obtained from spectroscopic analyses. The fact that S-nitrosylated enzyme can regain its activity upon addition of thiol compounds such as glutathione makes this class of compounds suitable as templates for the development of potent reversible and covalent cysteine protease inhibitors.

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Animals Humans Nitric Oxide Nitrosamines Proteins Journal Article Research Support, U.S. Gov't, P.H.S.

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Z Guo, N Miranda, P G Wang. 1999. Protein S-nitrosating agents.. https://doi.org/10.1016/s0076-6879(99)01089-7

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