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DNA preparation and efficient microsatellite analysis from insect hemolymph.

T Gerken·J Kurtz·K P Sauer·T Lubjuhn
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Abstract

A simple and time-saving method for DNA preparation for efficient microsatellite analysis is described. The method is based on thermal treatment of only 1-5 microL of insect hemolymph in a Chelex 100-suspension. Since hemolymph withdrawal does not harm the insects, analysis of mating systems, population structure and phylogenetic reconstruction can be conducted with minimal experimental influence.

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AnimalsDNAGenes, InsectHemolymphInsectaMicrosatellite RepeatsJournal ArticleResearch Support, Non-U.S. Gov't

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T Gerken, J Kurtz, K P Sauer, T Lubjuhn. 1998. DNA preparation and efficient microsatellite analysis from insect hemolymph.. https://doi.org/10.1002/elps.1150191804

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