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PubMed · 9987839

A consensus sequence for the Rhodospirillaceae SOS operators.

M Labazi·A del Rey·A R Fernandez de Henestrosa·J Barbé
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Abstract

The sequences controlling the expression of the Rhodobacter capsulatus recA and uvrA genes belonging to the SOS DNA repair system have been identified by PCR mutagenesis. Data obtained demonstrated that the GTTCN7GTAC and GAACN7GAAC motifs present upstream of the recA gene and the GTTCN7GTTC motif found upstream of the uvrA gene are required for their respective DNA damage-mediated induction. Alignment of recA promoters of R. capsulatus, Rhodobacter sphaeroides and Rhodopseudomonas viridis with the uvrA promoters of R. capsulatus and R. sphaeroides has identified the consensus sequence GTTCVYVYTWTGTTC as the SOS operator site of the Rhodospirillaceae family.

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Adenosine TriphosphatasesAmino Acid SequenceBacterial ProteinsDNA-Binding ProteinsEscherichia coli ProteinsGenes, BacterialMolecular Sequence DataMutagenesisOperator Regions, GeneticPolymerase Chain ReactionPromoter Regions, GeneticRec A RecombinasesRhodobacter capsulatusRhodospirillaceaeSOS Response, GeneticsJournal ArticleResearch Support, Non-U.S. Gov't

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M Labazi, A del Rey, A R Fernandez de Henestrosa, J Barbé. 1999-02-01. A consensus sequence for the Rhodospirillaceae SOS operators.. https://doi.org/10.1111/j.1574-6968.1999.tb13409.x

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