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PubMed1980

[Separation of lymphocytes by using visotrast 370 (author's transl)].

Enrichments of lymphocytes are mainly performed according to the flotation principle. By means of numerous differences with regard to the composition and density of the solution for separation, the relative acelleration and duration of the centrifugation the advantages and disadvantages of at least 7 methods were compared with our own modification: 1. Visotrast 370 diluted with Aqua dest. is equal to density gradients containing ficoll. 2. The one-step assays proved to be better and less time consuming than the combined methods. 3. With the density of the flotation medium of 1,076 g . cm-3 79 +/- 11% of the lymphocyte of the original blood were isolated, their purity was 93 +/- 3%, their vitality 91 +/- 4%. 4. There was slight contamination with other blood cells.

Acetrizoic Acid↗
PubMedDate not supplied

[Effect of contrast media on the structure and function of hepatocyte plasma membranes].

The cholecystographic radiopaque agents, adipiodon and endographin, unlike the urographic contrast agents, triombrin and iodamide, decrease the fluorescence intensity of hydrophobic probes 1-anilinonaphthalene-8-sulfonate and 4-dimethylaminochalcone in plasmic membranes (PM) of rat hepatocytes primarily at the expense of the lowering of the constant of probe association with the membranes. The similar but less marked effects were discovered in experiments with lecithin lyposomal preparations. As regards the intensity of action, cardiotrast and triotrast an intermediate position between the two groups of radiographic contrast agents. Adipiodon and endographin induce an inhibition of membranous Mg+2 (Na+, K+) ATPase, which correlates with their effect on the structure of the hepatocyte PM. The data obtained allow a conclusion that, unlike the urographic agents, the cholecystographic radiopaque agents are bound to the definite areas of the hepatocytic PM and provoke alterations in the activity of membranous APTase because of the conformational rearrangements of the membranes.

Acetrizoic Acid↗
PubMed1982

Comparative study: Endografine (diatrizoate), Vasurix polyvidone (acetrizoate), Dimer-X (iocarmate) and Hexabrix (ioxaglate) in hysterosalpingography.

Side effects of hysterosalpingography with Dimer-X, Hexabrix, Vasurix polyvidone and Endografine in 142 consecutive patients, receiving one of the four tested media were evaluated from replies to postal questionnaires. The Dimer-X group had a higher incidence of nausea and dizziness. The Endografine group had a higher incidence of abdominal pain. These differences occur especially in the age groups under 30 years. Hexabrix and Vasurix polyvidone are considered the best contrast media for hysterosalpingography and perhaps because of its low toxicity Hexabrix should be preferred.

Abdomen↗
PubMed1998

Transformation of the ionic X-ray contrast agent diatrizoate and related triiodinated benzoates by Trametes versicolor.

Iodinated X-ray contrast agents are considered to be nondegradable by microorganisms. The decomposition of the ionic X-ray contrast agents Diatrizoate (3,5-di(acetamido)-2,4,6-triiodobenzoic acid) and Iodipamide (3,3'-adipoyl-diimino-di(2,4,6-triiodobenzoic acid) and related triiodinated benzoates (Acetrizoate [3-acetylamino-2,4, 6-triiodobenzoic acid] and Aminotrizoate [3-amino-2,4, 6-triiodobenzoic acid]) by Trametes versicolor has been investigated. The fungus was able to transform all tested triiodinated benzoates cometabolically. During transformation of these compounds, iodide was released, but deiodination was not complete. T. versicolor liberated traces of 14CO2 from uniformly ring-14C-labeled Diatrizoate (3,5-di(acetamido)-2,4,6-triiodobenzoate). Various extracellular metabolites were detected during transformation of the different substances. In the transformation of Diatrizoate, the three main metabolites were identified as 3,5-di(acetamido)-2, 6-diiodobenzoic acid, 3,5-di(acetamido)-2,4-diiodobenzoic acid, and 3,5-di(acetamido)-2-iodobenzoic acid, suggesting reductive deiodinations in steps as initial transformation steps.

Benzoates↗