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Results for “AGGLUTININS AND AGGLUTINATION”

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PubMedDate not supplied

Elementary body agglutination for rapidly demonstrating chlamydial agglutinins in avian serum with emphasis on testing cockatiels.

The development and use of a stained chlamydial elementary body agglutination (EBA) antigen for detecting antibody activity in avian sera is described. Examples of serologic results on serum samples from various types of birds indicate the usefulness of EBA, latex agglutination (LA), and direct complement fixation (DCF) in diagnosing avian chlamydiosis. Results of tests on 10 cockatiels examined in clinics indicate that a combination of serology, culture, and/or antigen-detection enzyme-linked immunosorbent assay may be helpful when testing this type of bird. Agreement between EBA, LA, and DCF was 81.8% when testing 407 serum samples from cockatiels of unknown health status. The relationship between positive (> or = 10 titer) antibody activity and known health status of 77 cockatiels revealed that agreement between the two criteria was only 59.7%. Of 13 Chlamydia-inoculated cockatiels, seven birds seroconverted from negative to positive by EBA; five seroconverted by DCF. Only the five birds that seroconverted by both EBA and DCF were culture-positive for chlamydiae. None of 15 sham-inoculated control cockatiels developed detectable antibody activity, and none of 10 cultured were positive. In tests with column-separated IgM and IgG, EBA detected only IgM activity, LA detected IgM and IgG activity, and DCF detected only IgG activity.

Agglutination Tests↗
PubMed1984

A development-dependent hemagglutinin from cucumber surfaces: I. Hemagglutinin activity of cotyledon surfaces.

During studies on the chemistry of plant surfaces, we observed that concomitant with the development of cucumber (Cucumis sativus L. cv ;ELEM') cotyledons an agglutinin that agglutinates human erythrocytes appeared on epicuticular surfaces. The agglutinin was released from cotyledon surfaces into distilled water by a 1-minute immersion (or even less). Homogenization of the washed cotyledons released residual agglutinating activity. The surface-located hemagglutinating activity was age-dependent and occurred in dark- and light-grown cotyledons. Agglutinating activities were present in light-grown cotyledons of 2- to 14-day-old seedlings and in dark-grown cotyledons of 3- to 17-day-old seedlings. Agglutinating activities were maximal in cotyledons of 3- to 4-day-old seedlings. No activity could be detected in dry seeds or in seedlings up to 2 days and after 17 days of germination. The hemagglutinating activity was specifically inhibited by N,N',N''-triacetylchitotriose, chitin, and chitosan.

Journal Article↗
PubMedDate not supplied

The persistence of leptospiral agglutinins titers in human sera diagnosed by the microscopic agglutination test.

The persistence of agglutinins detected by MAT has created some problems to the interpretation of the results. The aim of this study was to examine the data of serology from 70 patients with serologically confirmed diagnosis of leptospirosis by during 3-13 months after being affected with leptospires in order to elucidate the interpretation of the persistence of agglutinins detected by MAT. Sixty-one patients sera (87.14%) had titers equal or greater than 800. Of these, two individuals maintained titers of 800 thirteen months after the onset. This study showed that only one sample of sera with high titers is not reliable to determine the time at which infection occurred.

Agglutination Tests↗
PubMed1965

DETECTION OF STREPTOCOCCAL GROUP-SPECIFIC ANTIBODIES IN HUMAN SERA.

Two serologically distinct antibodies directed against different antigenic sites on the Group A carbohydrate were detected in human sera by agglutination techniques. Agglutinins with A-variant specificity were demonstrable by the direct agglutination of purified A-variant cell walls, whereas indirect Coombs-type agglutinins with Group A specificity were demonstrable with Group A cell walls. Selective adsorption with homologous cell walls as well as inhibition studies with the soluble carbohydrates confirmed the specificity of the two types of agglutinins. There was an obvious correlation between the magnitude of the antistreptolysin O titer, and the direct Group A-variant and the indirect Group titers. These data suggest that indirect anti-Group A agglutinins and direct anti-Group A-variant agglutinins are produced following a Group A streptococcal infection.

Agglutination↗