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At least 181 records · Page 10Linked to original sources
PubMed1995

Definition and application of a fourier domain texture measure: applications to histological image segmentation.

A texture measure is defined for the purpose of two-dimensional histological image classification, based upon a novel exploitation of the modulus of the Fourier transform. Its implementation in the form of an algorithm is presented and discussed, and employed to classify a set of histological images. The results, which were obtained by the analysis and classification of different mammalian tissue types, compare favourably with established texture recognition techniques. Furthermore, the results suggest that the method has a comparable performance to other techniques at reduced storage and time costs for images of a high grey level resolution.

Algorithms↗
PubMed1996

Construction and application of a new class of sequential oligopeptide carriers (SOCn) for multiple anchoring of antigenic peptides--application to the acetylcholine receptor (AChR) main immunogenic region.

A new class of sequential oligopeptide carriers (SOCn), namely (Lys-Aib-Gly)n (n = 2-7), for anchoring antigenic peptides, is presented. These SOCn have been designed in order to assume a determined structural motif, exhibiting defined spatial orientations of the Lys-N epsilon H2 anchoring groups. The NMR study showed that SOCn adopt a rigid conformation with some regularity, initiated from the C-terminus of the carrier, while molecular dynamics simulation confirmed the occurrence of a distorted 3(10)-helix. It was also demonstrated, by 1HNMR, that all the antigenic peptides bound to the SOCn retain their original, folded active, structure and that probably they do not interact to each other. It is concluded that the beneficial structural elements of the SOCn impose a favorable disposition of the anchored peptides so that potent antigens with maximum molecular recognition are generated.

Antibodies, Monoclonal↗
PubMed1980

Thermodynamic nonideality and the dependence of partition coefficient upon solute concentration in exclusion chromatography. Application to self-associating and non-self-associating solutes. Application to hemoglobin.

The theory of partition of macromolecular solute between mobile and stationary phases under highly nonideal conditions is reexamined. Expressions are derived for the dependence of the partition coefficient of a non-self-associating solute, and the weight average partition coefficient of a dimerizing solute, upon the total concentration of solute in the mobile phase. These expressions are based upon a treatment of nonideality in the stationary phase which is more realistic than that proposed earlier [L.W. Nichol, R.J. Siezin and D.J. Winzor, Biophys. Chem. 9 (1979) 17]. It is found that the chromatographic data on oxyhemoglobin in the above paper may be accommodated by the present theory without invoking self-association.

Chromatography, Gel↗
PubMed1996

Stem cells from bone marrow, umbilical cord blood and peripheral blood for clinical application: current status and future application.

Bone marrow transplantation (BMT) has progressed rapidly during the past two decades to that of a treatment of choice as a therapeutically effective modality for the treatment of selected patients with malignant disease and non-malignant hematological disorders. However, its use is limited by availability of human leukocyte antigens (HLA)-matched donor cells, engraftment and graft-versus-host disease (GVHD). Prevention of GVHD, improvement in the speed and quality of marrow reconstitution, and screening of new immunomodulating agents which improve engraftment and augment hemopoiesis are intense areas of investigation. To this end there has clearly been progress in purification and characterization of human stem cells from different tissue sources. Discussed in this review are: (a) stem cell purification, characterization and ex vivo expansion; (b) bone marrow stem cell transplantation; (c) cord blood stem cell transplantation; (d) peripheral blood stem cell transplantation; (e) fetal liver stem cell transplantation; (f) in utero stem cell transplantation; and (g) evaluation of the capacity of stem cells to serve as targets for gene therapy.

Blood Cells↗
PubMed2006

The future of organ replacement: needs, potential applications, and obstacles to application.

The supply of organs available for transplantation is already far smaller than the demand, and the demand may grow substantially in the near future. For this reason, it is timely to consider how organ function might be replaced in the future. In this article, we consider new technologies that might be used to replace organ function, the obstacles to applying new technologies, and how those obstacles might be overcome in the development of new strategies for organ replacement.

Animals↗
PubMed2003

Immunocytochemical applications of specific antisera raised against synthetic fragment peptides of mummichog GtH subunits: examining seasonal variations of gonadotrophs (FSH cells and LH cells) in the mummichog and applications to other acanthopterygian fishes.

Two distinct types of gonadotrophs, FSH (GtH I) cells and LH (GtH II) cells, were immunocytochemically identified from mummichog (Fundulus heteroclitus; Cyprinodontiformes, Acanthopterygii) pituitary using antisera raised against synthetic fragment peptides of FSHbeta and LHbeta. Both cell types were abundant during the spawning period (spring and early summer) and decreased in number during the post-spawning immature period. The number of FSH cells increased again during the early phases of gonadal development (cortical alveoli accumulation in the oocytes and basal spermatogenesis) in early winter, whereas the number of LH cells did not. Only FSH cells were abundant during the latter phases of gonadal development (vitellogenesis and active spermatogenesis) in early spring. These observations suggest that both GtHs have important yet different roles for reproduction in this species. Antisera against the conservative region of the FSHbeta and the LHbeta subunits immunostained FSH cells and LH cells, respectively, also in red seabream (Pagrus major; Perciformes, Acanthopterygii) and small mouth bass (Micropterus dolomieu; Perciformes, Acanthopterygii), suggesting the possibility of their use for other acanthopterygian fishes.

Animals↗