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Biochemical basis of obligate autotrophy in Nitrosomonas europaea.

The specific activities of isocitric dehydrogenase, alpha-ketoglutaric dehydrogenase, succinic dehydrogenase, malic dehydrogenase, and reduced nicotinamide adenine dinucleotide (NADH) oxidase were determined in extracts of Nitrosomonas europaea and compared with the corresponding values for Anacystis nidulans and autotrophically grown Hydrogenomonas eutropha. In common with other obligate autotrophs and in contrast to facultative autotrophs, Nitrosomonas extracts lacked alpha-ketoglutaric dehydrogenase and KCN-sensitive NADH oxidase activity and had low succinic dehydrogenase activity. The Nitrosomonas NADH oxidase appeared to be of the peroxidase type.

Isocitrate Dehydrogenase↗

[Determination of the limits of the physiological action of mineral elements in hydrogen bacteria].

The growth of the hydrogen bacterium Hydrogenomonas eutropha Z-1 was studied in the conditions of continuous cultivation at different content of mineral elements in the medium. The physiological action of phosphorus, sulfur, potassium and magnesium in the bacterium was found to be displayed within a wide range of concentrations (mg/ml): P, 18-1400; S, 5.5-350; K, 4-550; Mg, 4.8-430. If the control of mineral elements was above or below these values, the specific growth rate of the cells decreased and their intracellular chemical composition changed.

Culture Media↗

Influence of Cosmos 368 space flight conditions on radiation effects in yeasts, hydrogen bacteria and seeds of lettuce and pea.

Radiobiological studies have been carried out on board the Cosmos 368 satellite, launched on 8 October 1970 and returning to earth on 14 October 1970. Yeast diploid cells Saccharomyces ellipsoides, Megri strain 139-B, haploid cells Zygosaccharomyces Baili, hydrogen bacteria Hydrogenomonas eutropha, strain Z-1, Berlin variety lettuce seeds and Capital variety pea seeds were used in these experiments. The biological specimens were irradiated with gamma-rays at dose rates of 71.8 and 6.7 rad d-1 suring the packaging of containers and after returning the samples to the laboratory. It was found that both on pre-radiation and post-radiation exposure space flight factors did not greatly influence radiobiological effects.

Cosmic Radiation↗

[Characteristics of a continous culture of hydrogen bacteria under conditions of gas limitation].

The effect of the specific growth rate and limiting gas on the concentration of cells, the rate of gas utilization, and the economic coefficients was studied during continuous cultivation of Hydrogenomonas eutropha Z-1. Cell concentrations as functions of the specific growth rate with limitation by H2, 02, and CO2, were plotted as hyperbolae and were linear in the coordinates X, 1/D. The rates of gas absorption Q (M/1 - hr) grow linerly with the concentration of the cells in the culture. The economic coefficients are linear in the coordinates 1/y, 1/micron. The effect of the specific growth rate and kind of limiting gas on the ratios between absorbed H2, 02 and C02 is described.

Carbon Dioxide↗

[Possibility of culturing hydrogen bacteria in the presence of CO].

The growth of Hydrogenomonas eutropha Z-1 was studied in the presence of CO in the gaseous phase. The culture was found to be resistant to the action of carbon monoxide at concentrations of 5 per cent (periodic cultivation) and 7 per cent (continuous cultivation). The hydrogen bacteria can assimilate CO as a source of carbon.

Absorption↗

[Hydrogen and carboxide bacteria belonging to the microflora of degradation].

The following bacteria oxidizing hydrogen and/or carbon monoxide were shown by cultivating in the concentration gradient of acetate, lactate, and glucose, to belong to the microflora of dispersal: Hydrogenomonas eutropha Z-1, H. pantotropha Z-11, Pseudomonas carboxydoflava Z-1107, Achromobacter carboxydus Z-1171, Paracoccus denitrificans, and Alcaligenes paradoxus.

Alcaligenes↗

CHARACTERIZATION OF POLY-BETA-HYDROXYBUTYRATE EXTRACTED FROM DIFFERENT BACTERIA.

Lundgren, D. G. (Syracuse University, Syracuse, N.Y.), R. Alper, C. Schnaitman and R. H. Marchessault. Characterization of poly-beta-hydroxybutyrate extracted from different bacteria. J. Bacteriol. 89:245-251. 1965.-Poly-beta-hydroxybutyrate (PHB) from different bacterial genera was studied with regard to its crystal structure, infrared absorption, intrinsic viscosity, and electron microscopy. All PHB samples precipitated from dilute chloroform solution gave identical X-ray diffractograms confirming uniformity of crystal structure, and uniformity of molecular structure, based on the similarity of the recorded infrared spectra, was also established. Crystal morphology was also similar, showing the reported "lath" shape structure for purified polymer from Bacillus cereus. Intrinsic viscosity ranged from 0.04 to 11.5 depending upon the polymer treatment; polymer molecular weights, based upon viscometry, could be estimated to range from 1,000 to 250,000. It is concluded that the same basic molecule is involved in all PHB present in the bacterial kindgom.

Azotobacter↗