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Control of food-borne infections and intoxications.

Most food-borne diseases are caused by microorganisms that initially contaminate the living plant or animal or recontaminate the food during handling or processing. Control measures are intended to (1) prevent microorganisms from contaminating food and involve all hygienic production measures (raw material, premises, equipment, cleaning and disinfection, personnel); (2) prevent microorganisms both from growing or forming toxins, e.g. through chilling, freezing or other processes that do not destroy microbes, such as reduction of aw or pH; (3) eliminate microorganisms, e.g. through thermal processing. Integrated systems for control of the microbiology and hygiene of foods aim at a gradual or stepwise reduction of health hazards at all stages of production and processing until final operations. As a further development of HACCP (Hazard Analysis Critical Control Point), other systems such as the LISA (Longitudinally Integrated Safety Assurance) concept, include also the primary stages of harvesting. With respect to food animals, this clearly involves veterinary control of the livestock as an initial stage of food production. As with salmonellosis, the implementation of control systems is helpful in reducing risks at particular critical points. However, isolated measures do not solve the problem as a whole and cannot meet the demands of consumer protection. At least in the case of salmonellosis a global strategy of control is needed which requires political decisions from the relevant public health bodies.

Animal Husbandry↗

Fuchs heterochromic cyclitis and ocular toxocariasis.

PURPOSE: To report the association of Fuchs heterochromic cyclitis (FHC) and ocular toxocariasis in a young adult. DESIGN: Observational case report. METHODS: A 26-year-old patient was referred for the management of a unilateral intermediate uveitis associated with a lower peripheral subretinal fibrotic lesion near the pars plana. Diagnosis of FHC was clinically confirmed. Laboratory examination was performed to exclude an infectious condition. RESULTS: LISA assay detected significant levels of IgG directed against Toxocara canis. Toxoplasmic serology was negative, excluding this differential diagnosis. Other examinations, including complete blood cell count, urinalysis, serum angiotensin-converting enzyme, lysosyme, chest CT scan, and syphilis serology were noncontributive. CONCLUSIONS: Previous studies have reported on the association of FHC and other parasitic conditions, such as toxoplasmosis but also on herpetic ocular infections. Serologic analysis for toxocariasis may be proposed in patients with FHC and retinal scars in the absence of toxoplasmosis.

Adult↗

The effectiveness of computer-aided learning in teaching orthodontics: a review of the literature.

INTRODUCTION: The purposes of this review were to evaluate the effectiveness of computer-aided learning (CAL) in orthodontic education, to make evidence-based recommendations for the use of CAL in orthodontics, and to develop guidelines for conducting comparative trials to evaluate CAL as a mode of learning in orthodontic education. METHODS: Medline, the Cochrane Library Database, ERIC, CINAHL, LISA, Psycinfo, and IPA were searched for randomized controlled trials evaluating the effectiveness of CAL in orthodontics. Outcome measures included objectively measured posttest scores on multiple choice, written, or oral tests; performance on a clinical procedure or clinical interview; time spent on CAL programs to learn the material presented; and responses to questionnaires conveying participants' attitudes toward various modes of learning. RESULTS: Four randomized controlled trials comparing CAL with conventional teaching fulfilled the inclusion criteria and met the cutoff quality assessment checklist (QAC) score of > or = 8. Each study was assessed for quality by 2 independent reviewers. The validity and strength of the selected studies were assessed by using a QAC for an educational intervention. CONCLUSIONS: The controlled trials of CAL in orthodontics that met our QAC cutoff score of 8 were split, with 2 showing that CAL enjoyed a significant advantage over conventional teaching, 1 showing no difference, and 1 showing that the conventional tutorial method was better. More high-quality trials evaluating the effectiveness of CAL in orthodontics are needed. CAL programs in orthodontics elicit mostly positive responses and attitudes from students toward learning.

Attitude of Health Personnel↗

Catalase binds Grb2 in tumor cells when stimulated with serum or ligands for integrin receptors.

Recent studies have demonstrated that H(2)O(2) acts as a second messenger of mitogenic signaling and that catalase is under the regulation of PKA and PKC signaling. Here we examined whether catalase binds any mitogenic signaling molecules. Our results indicated that serum stimulation of HeLa, Caco-2, and LiSa-2 cells, but not BJ-1 and primary human bronchial epithelial cells, resulted in catalase binding to Grb2. Whereas serum deprivation, butyrate, and herbimycin-A negatively regulated the binding, an extended culture of confluent Caco-2 cells resulted in binding of an additional but as yet unidentified molecule to the Grb2-catalase complex. Expression of active catalase nearly 15-fold over control level in Tet-off HeLa cells substantially increased binding to Grb2, and this was sensitive to 3-aminotriazole, a specific catalase inhibitor. Furthermore, fibrinogen, fibronectin, and laminin, but not collagen types I to V, hyaluronic acid, elastin, insulin, EGF, IGF-I, PDGF, or NGF, resulted in binding similar to that of serum. A mutation of tyrosine to phenylalanine at 447 abolished the binding capability of catalase to Grb2 in vitro. These results support the view that catalase (447)Tyr-Val-Asn-Val binds Grb2 upon phosphorylation in tumor cells when stimulated with serum or ligands for integrin receptors. This is the first report demonstrating that catalase binds a SH2 domain of the molecule and participates in integrin signaling.

Adaptor Proteins, Signal Transducing↗

Spinodal instability and pattern formation in thin liquid films confined between two plates.

The instability, morphology and pattern formation engendered by the van der Waals force in a thin liquid film of thickness h confined between two closely placed solid surfaces (at distance d > h) are investigated based on nonlinear 3D simulations. The initial and the final stages of dewetting and pattern formation are found to be crucially dependent on the volumetric (thickness) ratio of air and liquid and its deviation from the location of the maximum of the spinodal parameter versus volumetric ratio curve. On a low energy surface, relatively thinner films and wider air gaps favor initial dewetting of the lower plate by the formation of holes, whereas thicker films with thinner air gaps initially evolve by the formation of columns/bridges that join the upper plate. In the later stage of evolution, the initial holes in thinner films evolve into columns/drops, while a rapid coalescence of columns in the thicker films eventually causes formation of holes. Thus, a phase inversion, either from liquid-in-air to air-in-liquid dispersion or vice versa, occurs during the final stages of evolution. A thin film confined between two high-energy solid surfaces forms columns (bridges) only when its mean thickness, h0, is greater than a critical thickness (hc) or the air gap is smaller than a critical distance. The patterns can be aligned by using a topographically patterned confining surface. Conditions on pattern periodicity, amplitude, and the volumetric ratio of air and liquid in the gap are explored for the formation of various types of ordered patterns including annular rings of columns, concentric ripples, parallel channels and a rectangular array of complex features. The results are of significance in soft lithographies such as LISA, soft stamping and capillary force lithography.

Journal Article↗

[Auto-antibodies to anti-ENA SSA/RO (52 and 60 kDa): an auto-immunity laboratory's experience].

PURPOSE: Anti-SSA/Ro and anti-SSB/La autoantibodies are frequently encountered in SLE or SGS where anti-SSA subtypes 52 and 60 kDa seems to be differently found in connection with the disease type: anti-SSA/Ro 60 kDa more frequently found in SLE and anti-SSA/Ro 52 kDa in SGS. We try to find if it was interesting in identifying these specificities for all anti-ENA screening. METHOD: The study included 162 patients' sera found anti-SSA 52 and/or anti-SSA 60 and/or anti-SSB positive among 1600 screening tests from the different hospital's services. We used two assays: first, dotblot (Innolia-Ana Update INGEN) as a screening test and second, an Elisa (ENA-LISA BMD) as confirmation. Thirty-eight control sera were found negative with dotblot. RESULTS: Only one subtype of anti-SSA (52 or 60 kD) or anti-SSB was found for 55 sera (44 anti-SSA 52, 10 anti-SSA 60, 1 anti-SSB) and 107 sera were found positive for two or more (73 anti-SSA 52 + 60 and 34 anti-SSA 52 or 60 with another anti-ENA). While anti-SSA 60 kDa alone or not was always positive with the Elisa test, neither anti-SSA 52 alone was anti-SSA Elisa's positive. Diseases associations results show a greater linking of anti-SSA 60 kDa with SLE, a frequent linking of combined reactivity anti-SSA 52/60 in SLE and SGS and a greater spreading of anti-SSA 52 kDa alone among pathological groups, showing an autoimmune disease's linking in 68%. Among SGS, 29% had only anti-SSA 52 kDa. CONCLUSION: We suggest screening specific tests for identifying anti-SSA/Ro 52 kDa reactivity which are missed by routine testing (tests using animal's antigens) and could represent an additional serum marker in Connective Tissue Diseases.

Adolescent↗

Modelling and simulation of acoustic wave propagation in locally resonant sonic materials.

Sonic crystals are artificial structures consisting of a periodic array of acoustic scatterers embedded in a homogeneous matrix material, with a usually large impedance mismatch between the two materials. They exhibit strong sound attenuation at selective frequency bands due to the interference of multiply reflected waves. However, sound attenuation bands in the audible range are only achieved by unfunctionally large sonic crystals. If local resonators are used instead of simple scatterers, the frequencies of the attenuation bands can be reduced by about two orders of magnitude. In the present paper we perform numerical simulations of acoustic wave propagation through sonic crystals consisting of local resonators using the local interaction simulation approach (LISA). Three strong attenuation bands are found at frequencies between 0.3 and 6.0 kHz, which do not depend on the periodicity of the crystal. The results are in good qualitative agreement with experimental data. We analyze the dependence of the resonance frequencies on the structural parameters of the local resonators in order to create a tool for design and optimization of any kind of sonic crystal.

Journal Article↗

Geometrical basis of perception of gaze direction.

Perception of gaze direction depends not only on the position of the irises within the looker's eyes but also on the orientation of the looker's head. A simple analysis of the geometry of gaze direction predicts this dependence. This analysis is applied to explain the Wollaston effect, the Mona Lisa effect, and the newly presented Mirror gaze effect. In an experiment synthetic faces were used in which the position of the iris and the angle of head rotation were varied. Different groups of subjects judged iris position, head rotation, and gaze direction of the same stimuli. The results illustrate how cues of iris location and head orientation interact to determine perceived gaze direction.

Attention↗

Perspectives of a cardiac surgery resident in-training on off-pump coronary bypass operation.

BACKGROUND: Contemporary residency training in the cardiac component of cardiothoracic operation is focused mainly on attaining technical proficiency in coronary artery revascularization. Most trainees in cardiothoracic operation are required to perform 35 cases of coronary revascularization in order to fulfill the minimum requirements for certification by the American Board of Thoracic Surgery. Although experience in minimally invasive coronary revascularization is not required for board certification in cardiothoracic operation, it is recognized by both trainees and program directors as an important component of contemporary training in less-invasive surgical approaches (LISA) for coronary artery disease. The objective of this study was to describe the training of residents in off-pump coronary revascularization in an accredited training program. METHODS: A retrospective analysis of a single resident's experience in minimally invasive coronary revascularization over an 18 month period in a 2-year residency program in cardiothoracic operation was carried out. RESULTS: Of the 166 total cases of coronary bypass operation, the resident performed 61 cases (37% of the total) off-pump as primary surgeon with attending supervision. Patients undergoing off-pump coronary bypass had an average of 2.4 grafts (range 1 to 5) and conventional coronary artery surgeon (on cardiopulmonary bypass) performed by the resident had an average of 4 grafts (range 2 to 5). The marginal/circumflex system was bypassed in patients that required revascularization of the marginal/circumflex system, which was 56% (n = 36 of 62) in the off-pump group and 90% for the conventional group using cardiopulmonary bypass. The conversion rate from off-pump technique to conventional coronary bypass using cardiopulmonary bypass was 3.2% in this series. CONCLUSIONS: Technical innovations and evolution of techniques to better stabilize the heart for off-pump coronary revascularization have made the procedure both effective and safe. Our experience has shown that cardiothoracic residents can be taught the skills necessary to perform coronary revascularization off cardiopulmonary bypass. There are currently no standards for the training of cardiothoracic residents in off-pump coronary artery operation. We propose that at least 50 cases be performed under supervision by a trained surgeon to obtain adequate credentials in minimally invasive coronary revascularization.

Aged↗

Nanobalance: an automated interferometric balance for micro-thrust measurement.

The paper is concerned with a new instrument, nanobalance, measuring thrust and noise of space micro-thrusters in the range 0-1 mN with submicronewton accuracy. Low-noise micro-thrusters will be essential for upcoming "drag-free" space missions like GOCE, SMART-2, LISA, and Darwin. Nanobalance departs from traditional thrust measuring concepts so as to exploit sensitivity of in-vacuum Fabry-Pérot interferometers to subnanometric displacements. That is achieved by an optical cavity embraced by two equal pendulums suspended at a constant distance, one of which carrying the micro-thruster under test. Any thrust, changing the optical path length of the injected laser beam, is detected as the frequency variation restoring a standing wave in the cavity. The paper presents the measurement principles, the error budget as derived from recent tests as well as instrument automation, which is essential for facilitating instrument setup and adaptation from measurement trial to trial. The authors are responsible for instrument automation and measurement processing.

Journal Article↗

Modeling the propagation of ultrasonic waves in the interface region between two bonded elements.

An important task in nondestructive materials evaluation is the development of techniques to characterize the bond quality of adherent joints. Binding forces are nonlinear and cause a nonlinear modulation of transmitted and reflected ultrasonic waves. As a consequence, the higher harmonics generated by an insonified monochromatic wave give information about the adhesive bonds. The local binding forces in thin bonded interfaces can be obtained by the amplitudes of the ultrasonic waves of the insonified frequency and its higher harmonics as transmitted through the interface. Additional phase measurements may enable one to obtain the evaluation of the full hysteretic cycle of the interaction force. In order to gain a deeper understanding of the interface region and to improve the technique, numerical simulations of the ultrasonic wave propagation through specimens of two bonded elements can be used. A simple model based on the local interaction simulation approach (LISA) is described in this contribution, and a comparison between the results of the simulations and the experimental data is presented. Besides its intrinsic relevance for NDE, the problem considered in this paper may be very useful to analyze and test models for the simulation of ultrasonic wave propagation in nonclassical nonlinear mesoscopic elastic materials.

Journal Article↗

G protein-coupled-receptor cross-talk: the fine-tuning of multiple receptor-signalling pathways.

Signalling via the large family of G protein-coupled receptors (GPCRs) can lead to many cellular responses, ranging from regulation of intracellular levels of cAMP to stimulation of gene transcription. Members of this receptor family have been grouped into different categories dependent on the particular G protein subtypes that they predominantly interact with. Thus, receptors that couple to GS proteins will stimulate adenylate cyclase in many cells, while Gq/11-coupled receptors can mobilize intracellular Ca2+ via activation of phospholipase C. There is accumulating evidence, however, that activation of one particular signalling pathway by a GPCR can amplify intracellular signalling within a parallel but separate pathway. In this article Lisa Selbie and Stephen Hill review some of the evidence for these synergistic interactions and suggest that they may have an important role in finetuning signals from multiple receptor signalling pathways.

Animals↗

Pharmacological analysis of intracellular Ca2+ signalling: problems and pitfalls.

The complex changes in intracellular Ca2+ concentration that follow cell stimulation reflect the concerted activities of Ca2+ channels in the plasma membrane and in the membranes of intracellular stores, and the opposing actions of the mechanisms that extrude Ca2+ from the cytosol. Disentangling the roles of each of these processes is hampered by the lack of adequately selective pharmacological tools. In this review, Colin Taylor and Lisa Broad summarize the more serious problems associated with some of the commonly used drugs, and describe specific situations in which the multiple effects of drugs on Ca2(+)-signalling pathways have confused analysis of these pathways.

Animals↗

[Primary-care morbidity and true morbidity due to acute respiratory infections].

The present work presents the study of morbidity due to acute respiratory infections (ARI) in areas of the town of Lisa in Ciudad Habana, and Isla Juventud (Cuba), to characterize different aspects of morbidity measured by health care attendance and to measure true morbidity. About 90% of consultations for ARI were first-time consultations, while their ratio to further consultations was 5.3. True morbidity rates (TMR), obtained trough active research, ranged from 110.4 to 163.4 cases per 1000 inhabitants, considerably higher than morbidity rates measured by primary care consultations (MRPCC) in the same time period. The true morbidity index (TMI), as measured by the ratio of the two previous rates, ranged from 5 to 15. A high proportion (47.6%) of cases reported no medical care attendance. These results provide approximate estimates of true morbidity in the study area, and allow the establishment of a new control program, also improving epidemiologic surveillance within primary care activities.

Acute Disease↗

Reflections on mirror images. Neuropsychology.

About half of the readers of this article would not be able to tell whether a picture of the Mona Lisa is correct or mirror-reversed. Neuropsychological studies may help explain this striking dissociation between the ability to identify images and memory for their handedness.

Animals↗

Space bioreactors and their applications.

Space biology is a young and rapidly developing discipline comprising basic research and biotechnology. With the prospect of longer space missions and the construction of the International Space Station several aspects of biotechnology will play a prominent role in space. In fact, biotechnological processes allowing the recycling of vital elements, such as oxygen or water, and the in-flight production of food becomes essential when considering the financial and logistic standpoint. Every kilogram which, having been recycled or produced in space, does not have to be uploaded will drastically reduce the cost of space missions. In addition, the scientific community is offered a better opportunity to investigate long-term biotechnological processes performing experiments with a duration ranging from weeks to months. Therefore, there is an increasing demand for sophisticated instrumentation to satisfy the requirements of future projects in space biology. The carryover of knowledge from conventional bioreactor technology to miniature space bioreactors for a monitored and controlled cell culturing is one of the key elements for this new dimension in space life science. The first space bioreactors were developed and flown at the end of the last century. It has been demonstrated that cells of different types, from bacteria to mammalian cells, can be successfully grown in this type of culture vessel. This chapter presents different generations of bioreactors developed so far, their performances in space and their potential for the future, as well as the activities of the European Space Agency (ESA) in this domain. A dedicated chapter by Lisa Freed on the rotating wall vessel reactor and the latest NASA bioreactor research is also part of this volume.

Bioreactors↗

Kinetic and mechanistic study of the gas-phase reactions of a series of vinyl ethers with the nitrate radical.

NO(3) oxidation of methyl, ethyl, propyl, and butyl vinyl ethers has been studied under tropospheric conditions (atmospheric pressure and T = 293 +/- 3 K) in the LISA indoor simulation chamber. NO(3) was produced inside the reactor by thermal decomposition of N(2)O(5) previously added to the air-VOC mixture, and concentrations were monitored using FTIR spectrometry. All the kinetic experiments were carried out by relative rate technique using isoprene as reference compound, leading to the rate constants k(1) = (7.2 +/- 1.5) x 10(-13), k(2) = (13.1 +/- 2.7) x 10(-13), k(3) = (13.3 +/- 3.0) x 10(-13), and k(4) = (17.0 +/- 3.7) x 10(-13) cm(3) molecule(-1) s(-1) for methyl, ethyl, propyl, and butyl vinyl ethers, respectively. Main oxidation products have been identified like being formaldehyde and respectively methyl, ethyl, propyl, and butyl formates. Production yields of oxidation products were close to 50%. Oxygenated nitrates and peroxynitrates were also detected.

Free Radicals↗

Relational integration, inhibition, and analogical reasoning in older adults.

The difficulty of reasoning tasks depends on their relational complexity, which increases with the number of relations that must be considered simultaneously to make an inference, and on the number of irrelevant items that must be inhibited. The authors examined the ability of younger and older adults to integrate multiple relations and inhibit irrelevant stimuli. Young adults performed well at all but the highest level of relational complexity, whereas older adults performed poorly even at a medium level of relational complexity, especially when irrelevant information was presented. Simulations based on a neurocomputational model of analogical reasoning, Learning and Inference with Schemas and Analogies (LISA), suggest that the observed decline in reasoning performance may be explained by a decline in attention and inhibitory functions in older adults.

Adolescent↗