PubMed Health⌕ Search

SEARCH · PubMed Health

Results for “Microscopy, Polarization”

Explore indexed PubMed citations for clinical trials, systematic reviews and public health research. Read source abstracts and follow each citation to its original PubMed record.

Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.

At least 181 records · Page 10Linked to original sources
PubMed2006

Three-dimensional, spin-resolved structure of magnetic vortex and antivortex states in patterned Co films using scanning ion microscopy with polarization analysis.

Scanning ion microscopy with polarization analysis is utilized for three-dimensional spin mapping of the surface magnetization (SM) of circular Co dots created in situ by focused ion beam etching of 30 nm thin Co/Si(100) films. From 3D scanning ion microscopy with polarization analysis spin maps, direct evidence is found for the existence of vortex-antivortex states with in-plane circular or hyperbolic SM components and a wide core with perpendicular SM components which oscillate in the outer region and become zero.

Journal Article↗
PubMed2000

Detection of adulteration of locust bean gum with guar gum by capillary electrophoresis and polarized light microscopy.

Capillary electrophoresis (CE) and polarized light microscopy (PLM) were utilized in the detection of the adulteration of locust bean gum with guar gum. For CE analyses, standards of locust bean and guar gums were extracted with 30% CH3CN, removing the residual proteins from the gum matrix. A 8.75 mM NaH2PO4-20.6 mM Na2B4O7 buffer, pH 9, was used to separate these proteins and to identify marker proteins that were present in the guar gum. These markers did not co-migrate with components in the extracts of mechanically processed locust bean gum, and are used as indicators of adulteration. Using PLM with toluidine blue and iodine staining techniques, unadulterated locust bean gum samples were distinguished from mixed samples through the differential staining of components in locust bean versus guar and tara gums. These experiments in the use of CE and PLM provide orthogonal and complementary methods for the verification of 'true' positives and the elimination of 'false' positives.

Electrophoresis, Capillary↗
PubMed2004

Immunohistochemical collagen analysis of the most superficial layer in adult articular cartilage.

We investigated immunohistochemically the collagen type of the most superficial layer in 10 normal adult human articular cartilage specimens obtained from eight femoral heads and one each of the femoral condyle and the talus using routine light microscopy and polarizing microscopy. A membrane-like structure with strong bire-fringence covering the articular surface was observed under polarizing microscopy in each specimen. This structure was stained with anti-type I and anti-type III collagen antibodies but not with anti-type II collagen antibody. This immunohistochemical finding was identical to that in synovial tissue. The results of this study confirm that the most superficial layer of adult normal articular cartilage consists not of type II collagen but of types I and III, and that this layer is absolutely independent from its deeper layer.

Adult↗
PubMed1984

Demonstration of lung type II cell differentiation by polarized light microscopy.

Birefringence is shown to be a property of lamellar bodies in living lung type II cells and is used to demonstrate the appearance of differentiated type II cells in cultures of fetal rat lung explanted as early as Day 13. The culture medium was F-12 K without added serum, hormones, or growth factors. This system is considered to be potentially useful in analyzing factors and cell interactions involved in determination and cytodifferentiation of the type II cell from lung bud epithelium.

Animals↗
PubMed2002

Shearing microscopy using polarized optical microscope with shear stage and spectral analyser to study liquid crystalline polymers.

In-situ polarized optical microscopy using a shear stage and a spectral analyser as well as a CCD camera were applied to study the phase transition under shear flow for a thermotropic and side-chain-type liquid crystalline polysiloxane. The onset of the appearance of anisotropic texture of the polysiloxane was observed under shear flow using the CCD camera at temperatures much higher than the isotropic-liquid crystalline phase transition temperature if the polysiloxane was cooled from the isotropic phase in the quiescent state. Both the onset temperature and the temperature for full development of the anisotropic texture across the field of view became higher as the shear rate increased. The transmitted light intensity was also measured using a spectral analyser with crossed polarisers at wavelengths from 300 nm to 800 nm, and the integrated intensity of the spectrum was calculated. Changes in the spectrum and the integrated intensity against temperature in the cooling process were compared with observation using the CCD camera. Temperature dependence of the integrated intensity showed that the onset of the appearance of the anisotropic texture under high shear rates was detected at temperatures slightly higher than that observed using the CCD camera.

Journal Article↗
PubMed1990

[Stratification of the experimental incipient caries (polarized light microscopy and microradiography)].

The enamel caries establishes when at least a part of enamel dissolves by the action of cariogenic bacteria which produce insoluble glucan and lactic acid. The experimental caries should be prepared at the condition which simulates the natural environment. The author made 1 mm thick sections from 29 teeth extracted for convenience of orthodontic reason. The surface was covered except the "window" which will be exposed to the acid milieu. The samples were immersed for 4 to 120 days in the gelatin added lactate buffer of pH 4.5, pH 5.0, and pH 5.5. Ground sections were made after the experiment. The structure of the experimental incipient caries was described after Darling (1963); surface layer, body of the lesion, dark zone, and translucent zone. When the section was immersed for shorter period in the buffer of pH 4.5, the lesion was made up only two layers, namely, the surface layer and the body of the lesion. This type of lesion will be called Type I, hereafter. When the action of acid was more intense, only one layer, the body of the lesion remained after the dissolution of the surface layer. This type of lesion will be called Type II. The lesion which provided with all 4 layers will be called as Type III, and the lesion which was made up of 3 layers which remained after dissolved surface layer, will be called as Type IV. Both Type III and Type IV were established after immersing for longer period in pH 5.5, although they could be observed in groups which immersed in lower pH. Of the samples examined, a few example showed a five-layered structure; the surface layer, the first body of lesion, the first dark zone, the second body of lesion, the second dark zone, and the translucent zone. This phenomenon suggests that the speed of caries development was not uniform throughout the experiment. The relationship between the depth of the lesion, and the pH and the duration of acid action was studied. In the pH 5.0 group, the development of caries lesion was temporarily arrested between 30 and 60 days. This phenomenon would be an explanation to the formation of five-layered lesion.

Dental Caries↗