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In vivo microdialysis of corticotropin releasing factor (CRF): calcium dependence of depolarization-induced neurosecretion of CRF.

Corticotropin releasing factor (CRF) is a large neuropeptide which functions as a major neurotransmitter in physiological stress responses. We have developed a microdialysis method for detecting CRF release from the median eminence of anesthetized rats. Depolarizing concentrations of KCl increased release of CRF into the perfusion media; this effect was inhibited by 100 microM verapamil. Our characterization of the physiologic conditions of KCl-induced release of neuronal CRF using the microdialysis technique provides evidence that the CRF release system is Ca(2+)-dependent and maintains its integrity over many hours in anesthetized rats.

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Neurosecretion.

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