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PubMed1966

Pasteurella pestis detection in Fleas by fluorescent antibody staining.

In an effort to develop a method for the rapid field identification of plague-infected fleas, the authors have studied the feasibility of direct fluorescent antibody staining of the midgut contents of fleas fed on mice infected with Pasteurella pestis. Fluorescent antibodies prepared from antisera derived from rabbits inoculated with the water-soluble P. pestis fraction 1b antigen, the somatic antigen of heat-killed P. pestis (Bryans strain), and live avirulent (strain A1122) or virulent (Yreka strain) plague vaccines were used used in this study.This direct staining method proved to be impracticable, but encouraging results were obtained by fluorescent antibody staining of broth cultures of macerates of infected fleas after 24-48 hours' incubation.The broth enrichment technique has not yet been evaluated in the field, but it is expected to be of value since it is relatively simple to perform and requires only material that can easily be transported to remote areas.

Animals↗
PubMed1967

A transport medium for specimens containing Pasteurella pestis.

A medium, originally designed by Stuart and co-workers and later modified by Cary & Blair, for the maintenance and transport, without multiplication, of pathogenic bacteria contained in bacteriological specimens was tested in the laboratory and in the field in Viet-Nam to determine its effectiveness in preserving specimens known to contain Pasteurella pestis.The results indicate that this medium should be useful in diagnostic plague studies in areas where transport facilities are inadequate. Properly collected clinical specimens, sent to a central laboratory by any means and under any climatic conditions likely to be encountered in the hot tropics, should yield viable Pasteurella pestis for at least 30 days.

Culture Media↗
PubMed1969

Enhanced recovery of airborne T3 coliphage and Pasteurella pestis bacteriophage by means of a presampling humidification technique.

This paper reports a series of experiments in which two methods of collecting airborne bacteriophage particles were compared. A standard aerosol sampler, the AGI-30, was evaluated for its competence in measuring the content of bacteriophage aerosols. It was used alone or with a prewetting or humidification device (humidifier bulb) to recover T(3) coliphage and Pasteurella pestis bacteriophage particles from aerosols maintained at 21 C and varied relative humidity. Collection of bacteriophage particles via the humidifier bulb altered both the initial recovery level and the apparent biological decay. Sampling airborne bacteriophage particles by the AGI-30 alone yielded data that apparently underestimated the maximal number of potentially viable particles within the aerosol, sometimes by as much as 3 logs.

Aerosols↗
PubMed1966

An evaluation of Pasteurella pestis fraction-1-specific antibody for the confirmation of plague infections.

Owing to the relatively long duration of specific serum plague antibodies in the host, the serological diagnosis of plague has substantial advantages over the conventional method of isolation of the causative agent. For the performance of both the complement-fixation (CF) and passive haemagglutination (HA) tests, the use of highly purified, specific Fraction 1 antigen is essential, and its preparation is discussed in this paper. Although both tests measure the same antibodies, the passive HA test is the more sensitive and constitutes the most effective means of detecting recovery from plague infection in nature. The CF test is nevertheless invaluable for such purposes as determining the antibody content of sera from immunized or infected hosts, indicating the amount of Fraction 1 developed by strains of Pasteurella pestis in cultures and therefore present in a vaccine, or detecting and estimating the amount of Fraction 1 antigen in extracts prepared from infected animal tissues and therefore for the field diagnosis of wild rodent plague after contamination or decomposition of rodents' carcasses.

Animals↗