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Sex differences in heritability of sensitization to Blomia tropicalis in asthma using regression of offspring on midparent (ROMP) methods.

A genetic basis for asthma- and atopy-related quantitative traits, such as allergen-specific immunoglobulin E (IgE) levels, has been suggested by the observed familial aggregation of these traits in temperate climates. Less information is available for tropical climates, where different allergens may predominate. Sensitivity to the mite Blomia tropicalis is related to asthma in tropical climates, but heritability of B. tropicalis sensitivity and the impact of age, sex, and other environmental covariates on heritability have not been widely explored. Total and specific IgE levels were measured by immunochemiluminescent assay in 481 members of 29 Barbadian families (comprised of 340 parent-offspring trios or pairs) ascertained through two asthmatic siblings. Trait heritability was estimated using regression of offspring on mid-parent (ROMP) and pairwise correlation analysis of unadjusted IgE levels and on residual values after adjustment for covariates. Heritability of IgE levels to the major antigen of B. tropicalis (Blo t M) estimated by ROMP in 180 complete parent-offspring trios was 0.56. Heritability was consistently greater for male offspring than for female offspring. Similar sex-specific patterns were observed for specific IgE to Dermatophagoides pteronyssinus and total IgE levels and were relatively unaffected by adjustment for covariates. Pairwise correlational analyses of specific and total IgE levels showed similar results. Moderate heritability of Blo t M IgE levels was detected in these Barbadian families and was greater for sons than daughters. Adjustment for covariates had minimal impact. This suggests that future investigations of genetic determinants of IgE levels should include approaches that allow for potential sex differences in their expression.

Acari↗

Influence of animal manures on the biology of temperate earthworm, Eisenia fetida in tropical semiarid climate.

For understanding the potential utility in field scale production of vermicompost and vermiprotein economically, Eisenia fetida was cultured to establish the influence of (i) prevailing tropical semiarid (North-East region of Karnataka, India) environmental factors and (ii) different animal manures (cattle, horse and 1:1 mixture of cattle and horse) (on its growth, reproduction and life span. In three forms of diet, growth (mg/d/g live weight of worm) was almost similar, but the biomass in cattle manure (565.7 +/- 15.3) was significantly more than horse manure (494.9 +/- 22.8) and 1:1 mixture (470.3 +/- 22.0). Mean cocoon production (per worm/week) in horse manure (0.16) was significantly (P<0.001) lower than that in cattle manure (1.6) and in 1:1 mixed manure (1.4). Cattle favoured biomass, growth and cocoon production. Horse manure inhibited cocoon production and the conserved energy in this process might have been added to the body weight almost equal to that in cattle manure. In 1:1 mixed manure, the biomass was less due to moderate cocoon production (presumably due to the stimulatory influence of 50% cattle manure) under semifavorable nutrients and environmental conditions. Fecundity of the worms declined with aging, despite favourable nutrients and environmental conditions. Worms survived up to 92, 68 and 66 weeks in cattle manure, 1:1 mixed manure and horse manure respectively.

Animals↗

Analysis of the egg production pattern in a tropical wet climate: influences of the phase of production and the estimated period of oviposition.

The egg production data of a commercial strain of 400 Shaver laying hens were used to study the changes in the percentage/hen/day production from 1 to 48 weeks in lay, applying both simple linear and logarithmic models, which corresponded best to the real conditions. The maximum rate of egg production occurred in the first 12 weeks of lay, followed by a gradual decrease, which became significantly pronounced in the 25 to 48 week production phase. As the hens advanced in age, significant changes were observed in the trend and period of oviposition indicative of an increasingly delayed laying period.

Aging↗

[The role of cardiovascular diseases among the reasons for the transfer ahead of schedule of specialists working in the tropics].

The authors have considered the causes of inability to continue working in tropic climate because of health deterioration for 359 specialists and 19 lethal cases. The main cause of inability to work in tropical climate and primary cause of death was cardiovascular disease. One-third of the cases were due to exacerbation of chronic diseases. The exacerbations occurred more frequently in the most intensive phases of adaptation. Measures which should be taken to prevent breakdown of health in subjects working in tropical conditions are recommended.

Adaptation, Physiological↗

Sperm motion characteristics of GarolexMalpura sheep evolved in a semi-arid tropical environment through introgression of FecB gene.

The arid and semi-arid tropical climates of India are endowed with vast diversity of non-prolific sheep breeds. The GarolexMalpura sheep has been evolved in a semi-arid tropical environment by introgression of FecB gene via artificial insemination of Malpura ewes using diluted semen of prolific microsheep Garole and subsequently multiplied by inter se mating among GarolexMalpura halfbreds. The aim of the present study was to identify FecB mutation in sexually mature GarolexMalpura rams by forced RFLP-PCR of BMPR-1B gene and evaluate: (i) semen production and sperm motion characteristics of GM rams and (ii) influence of age and FecB genotype on their semen attributes. Semen was collected during autumn season from 12 donor rams by artificial vagina on 8 occasions at weekly interval. The overall means of traits which did not differed significantly with age or FecB genotyping were volume (0.72 ml), mass motility (4.44), sperm concentration (2721.56 x 10(6)ml(-1)), curvilinear velocity (134.51 microm/s), motility (81.3%), amplitude of lateral head displacement (6.24 microm), beat frequency (44.43 Hz), sperm head elongation (48.9%) and sperm head area (10.01 microm(2)). The FecB genotyping had a significant effect (P<0.05) on percent linearity and rapid motile sperms, which did not vary significantly with age. Although sperm concentration was higher in FecB(BB) and FecB(B+), compared to FecB(++) genotypes but the effect was non-significant. The age and FecB genotyping had significant effects (P<0.05) on straightness, average path velocity, straight-line velocity and percentage of medium or slow motile sperms. It is concluded that GarolexMalpura rams with introgressed FecB gene are capable of producing good quality semen in a semi-arid tropical climate.

Aging↗

The debate about acclimatization in the Dutch East Indies (1840-1860).

Around the middle of the nineteenth century, conflicting views were put forward on the influence of climate on health and disease in the Dutch East Indies. In this part of the world, old Hippocratic ideas influenced views on the cause of disease much longer than in the Netherlands. Moreover, Brunonian theories--which had been discarded in the Netherlands--fitted the discussion about the effect of temperature on the body. Additionally, scientific medicine was introduced. Scientific methods, such as the collection of meteorological and statistical data, were promoted by a small group of military health officers. However, the use of scientific data did not guarantee a clear-cut opinion on the causes of disease. Numbers proved as disputable as other, less objective, medical observations. Mortality statistics and numbers of patients especially were used as arguments in various discussions. The example of Bosch, who changed his views on the dangers of tropical climate, demonstrates that statistics could be used for different purposes. At first, in his position as General Inspector, he used them as an argument to provide better care for the military personnel; later, when retired and a civilian, he used them as justification for colonization in relation to an intended improvement of the living conditions of the natives. The dangers of tropical climate for the health of Europeans were played down as soon as other--primarily economic--motives for living in the tropics became strong enough.

Acclimatization↗

Post harvest spoilage of sweetpotato in tropics and control measures.

Sweetpotato storage roots are subjected to several forms of post harvest spoilage in the tropical climate during transportation from farmers' field to market and in storage. These are due to mechanical injury, weight loss, sprouting, and pests and diseases. Sweetpotato weevil is the single most important storage pest in tropical regions for which no control measures or resistant variety are yet available. Several microorganisms (mostly fungi) have been found to induce spoilage in stored sweetpotatoes. The most important among them are Botryodiplodia theobromae, Ceratocystis fimbriata, Fusarium spp., and Rhizopus oryzae. The other less frequently occurring spoilage microorganisms include Cochliobolus lunatus (Curvularia lunata), Macrophomina phaseolina, Sclerotium rolfsii, Rhizoctonia solani, Plenodomus destruens. Microbial spoilage of sweetpotato is found associated with decrease in starch, total sugar, organic acid (ascorbic acid and oxalic acid) contents with concomitant increase in polyphenols, ethylene, and in some instances phytoalexins. Several methods are used to control microbial spoilage. Curing to promote wound healing is found as the most suitable method to control microbial spoilage. Curing naturally occurs in tropical climates where mean day temperature during sweetpotato harvesting season (February-April) invariably remains at 32-35 degrees C and relative humidity at 80-95%. Sweetpotato varieties varied in their root dry matter content, and low root dry matter content attributed for their high curing efficiency. Curing efficiency of varieties also differed in response to curing periods. Fungicide treatment, bio-control, gamma irradiation, hydro warming, and storage in sand and saw dust were found to have intermediate impacts in controlling spoilage and enhancing shelf life of sweetpotato roots. Breeding program has to be chalked out to develop new varieties suitable to curing under tropical conditions in addition to developing varieties having multi-spectrum resistance to major post harvest rot pathogens and sweetpotato weevils.

Food Contamination↗

Climatic impact of tropical lowland deforestation on nearby montane cloud forests.

Tropical montane cloud forests (TMCFs) depend on predictable, frequent, and prolonged immersion in cloud. Clearing upwind lowland forest alters surface energy budgets in ways that influence dry season cloud fields and thus the TMCF environment. Landsat and Geostationary Operational Environmental Satellite imagery show that deforested areas of Costa Rica's Caribbean lowlands remain relatively cloud-free when forested regions have well-developed dry season cumulus cloud fields. Further, regional atmospheric simulations show that cloud base heights are higher over pasture than over tropical forest areas under reasonable dry season conditions. These results suggest that land use in tropical lowlands has serious impacts on ecosystems in adjacent mountains.

Altitude↗

Sperm motion characteristics of Garole rams raised for a prolonged period in a semi-arid tropical environment.

The Garole is a prolific but less well known and rare breed of small sheep found in the hot and humid Sunderban region of West Bengal. An ability to breed throughout the year and to graze in knee-deep water, resistance to foot rot and a strong mothering instinct are some of the special features of this breed. Garole rams could provide germplasm to incorporate prolificacy traits by artificial insemination of the nonprolific sheep breeds found in abundance in the semi-arid and arid tropical climates of India. The aim of the present study was to evaluate the semen production by Garole rams maintained in a semi-arid climate for three years and to objectively assess their semen quality by a computer-assisted sperm analysis technique. The donor rams were randomly selected each year from the original flock procured from their natural habitat or from the offspring born at the Institute farm. Semen was collected weekly for three weeks each autumn for three consecutive years from 8 rams each year. The overall means (SD) of the traits that did not differ significantly with age or year were volume, concentration, curvilinear velocity, average path velocity, amplitude of lateral head displacement, beat frequency, motility and the percentages of rapid motile sperms and of slow motile sperms. The age of the rams had a significant effect (p<0.05) on the straight-line velocity but this was not significantly affected by the length of exposure to the semi-arid climate. However, the age and year had significant effects (p<0.05) on linearity, straightness and the percentage of medium motile sperms. It was concluded that Garole rams are capable of producing good-quality semen even after a prolonged period of exposure to a semi-arid tropical climate.

Animals↗

The shelf life of some antibiotic preparations stored under tropical conditions.

Accelerated storage tests have been carried out to predict the shelf life of several commercial preparations stored directly under temperate and tropical climatic conditions. Two brands each of sulphathiazole tablets, tetracycline hydrochloride capsules and chloramphenicol capsules were studied over a period of three months. Shelf lives ranging from 6.2. to 29.7 months were predicted for temperate storage conditions (20 degree C/31% R.H.), whilst these periods were reduced giving values from 3.8 to 13.9 months for tropical conditions of 30 degree C/31% R.H and from 2.5 to 9.2. months when stored at 30 degree C/75% R.H. A decrease in the rate of drug dissolution was also observed for preparations stored directly under conditions of elevated temperature and humidity. The results indicate the need for stringent control over storage conditions for antibiotic preparations in tropical climates to ensure adequate drug stability and dissolution characteristics.

Anti-Bacterial Agents↗

Microbial keratitis in Hong Kong: relationship to climate, environment and contact-lens disinfection.

Microbial keratitis has been studied in Hong Kong as a representative sub-tropical climate of south China. An 18-month investigation in 1997/98 of 223 cases of ulcerative keratitis (presumed microbial) was conducted in the 2 million population of Shatin and Kowloon at the Prince of Wales and Hong Kong Eye Hospitals respectively with comprehensive microbiology. A case-control study was pursued at the same time between 45 contact-lens wearers (CLW) developing microbial keratitis and 135 lens-wearing volunteers matched for age, sex, educational status and visual acuity. Home water supplies were sampled for Acanthamoeba. Previous ocular surface disease and trauma (preventable by wearing goggles for grinding) were common predisposing causes while cosmetic wear of contact lenses was responsible for 26% of cases overall. Pseudomonas aeruginosa was the commonest bacterium isolated, from both CLW and non-CLW, with infection being acquired within the community. These 28 pseudomonads remained fully sensitive to the third-generation cephalosporins, aminoglycosides and quinolone antibiotics, which is very encouraging. Fungi were isolated, predominantly Fusarium sp., but less commonly than expected. A fungal/bacterial ratio was obtained of 1/17, while in comparison, the expected ratio for a tropical climate ranges from 1/5 (Singapore) to 1/2 (South India). Acanthamoeba was the second commonest microbe isolated from keratitis of CLW. The domestic water environment of 8% of homes of both patients and controls wearing contact lenses was colonized with Acanthamoeba. Lack of hygiene, use of tap water for storing lenses, failure to air-dry lens-storage cases or use of one-step hydrogen peroxide disinfectant were identified as risk factors for keratitis in CLW. The study results commend use of multipurpose solutions by CLW in Hong Kong to achieve the lowest expected rates of infection.

Acanthamoeba Keratitis↗

Abrupt climate change at the end of the last glacial period inferred from trapped air in polar Ice

The last glacial period was terminated by an abrupt warming event in the North Atlantic approximately 15,000 years before the present, and warming events of similar age have been reported from low latitudes. Understanding the mechanism of this termination requires that the precise relative timing of abrupt climate warming in the tropics versus the North Atlantic be known. Nitrogen and argon isotopes in trapped air in Greenland ice show that the Greenland Summit warmed 9 +/- 3 degrees C over a period of several decades, beginning 14,672 years ago. Atmospheric methane concentrations rose abruptly over a approximately 50-year period and began their increase 20 to 30 years after the onset of the abrupt Greenland warming. These data suggest that tropical climate became warmer or wetter (or both) approximately 20 to 80 years after the onset of Greenland warming, supporting a North Atlantic rather than a tropical trigger for the climate event.

Journal Article↗

Relationships between ovulation rate and litter size in purebred Landrace and Yorkshire gilts.

The aim of the present study was to investigate the ovulation rate and its relationship to number of total piglets born in purebred gilts under tropical climatic conditions. This study was conducted in two swine breeding herds (A and B) in the northeastern part of Thailand. The sources of swine genetic material originate from West Europe. Gilts were mated (AI) on the second or later observed estrus at a body weight of at least 130 kg. In most cases, they were mated at third estrus. One hundred and twenty-seven gilts, 24 Landrace and 24 Yorkshire from herd A, and 42 Landrace and 37 Yorkshire from herd B were used. Gilts were examined once by laparoscopy under general anesthesia between days 8 and 15 after mating. The ovaries were examined and the pathological findings were recorded. The number of corpora lutea was counted, and was assumed to equal the ovulation rate. Subsequent mating results and farrowing data were recorded. The data were analyzed with analysis of variance. Single or double unilateral cysts and par-ovarian cysts did not affect mating results. Landrace gilts were significantly younger at first mating than Yorkshire gilts (244 versus 249 days, P < 0.05). At first mating, Yorkshire gilts had a significantly higher ovulation rate compared to Landrace gilts (15.3 versus 13.8, P < 0.001). There was no difference in the number of total piglets born per litter between the two breeds, but the total prenatal loss from ovulation to farrowing was significantly higher in Yorkshire than in Landrace gilts. Both the low ovulation rate and the high prenatal loss contribute to the low litter size in gilts raised under tropical climatic conditions.

Aging↗

[The sleep of Africans and Europeans in the Ivory Coast: questionnaire study].

Sleep behaviour has been extensively studied with questionnaires in industrialised countries to investigate the epidemiology of sleep-wake disorders. However, only few attempts have yet been made to examine sleep behaviour of people living in Africa. Although, a large number of studies in hot or cold environments have used short-term exposures, reporting disrupted sleep for most of them, long-term exposures to stressful thermal environments are rare in the literature. Prior to the present investigation, we used questionnaires to analyse the effects of seasonal heat increase on perceived sleep behaviour and sleep quality in young native African students in Niger [7], even though these methods of investigation are by no means as accurate as polysomnographic recordings. The hypothesis was that sleep behaviour may be influenced by climatic variations in a hot dry tropical climate. Such climatic variations have been shown to induce seasonal heat acclimatisation marked by changes in body temperature rhythms in the hot versus the cool season [13]. Sleep behaviour was examined during two 7-day periods in January ("cool-dry" season, 88 subjects) and May ("hot-dry" season, 53 subjects). The questionnaire was completed after night sleep and/or naps. The subjects slept an average of 7 1/2 hours a day, most of them having afternoon naps. They experienced no major seasonal variation in their sleep behaviour, but for an increased number of awakenings during the hot season. Restorative quality of sleep scored lower after a nap than after nocturnal sleep. Therefore, general sleep characteristics were not modified by seasonal temperature variations in African native students, perhaps because of the limited changes in daylight under the low latitude of Niamey. Another investigation was carried out using the same 12-item questionnaire in Abidjan on 78 medical students who did not have a nap [9]. Contrary to the Niamey students, the Abidjan subjects adopted short duration sleep schedules, without any effect on the subjective quality of the restorative properties of their sleep.

Adult↗

Health surveillance of Glasgow medical undergraduates pursuing elective studies abroad (1992-1998).

BACKGROUND: An integral part of the training for many UK medical undergraduates involves a period of elective study abroad. There is concern about the health risks this poses to the students, and uncertainty regarding the responsibility this places on medical schools. METHODS: Annually since 1992, medical undergraduates at Glasgow University have been asked to complete and return a confidential questionnaire on return from their elective studies. This records personal demographic details, the countries visited, and information about illnesses experienced. Analyses were conducted on the students' health experiences, lifestyle, the health precautions taken, and the climates experienced. RESULTS: Global statistics were compiled on 750 respondents. A subset of 267 completed a more extensive, post-1996, questionnaire enabling detailed study of comparative illness rates. A majority took pretravel health advice, visited only one country, stayed for 1 to 2 months, and experienced a tropical climate. Forty-five percent reported symptoms of illness, and alimentary symptoms predominated (77% of those ill). Higher illness rates were reported in those who experienced a hot desert or tropical climate compared with those who did not. There was correlation between taking professional pretravel health advice and exposure to a more hazardous climate. CONCLUSIONS: The attack rate for medical students on electives compares favorably to that for package holidaymakers; similarly the attack rate for students staying in the tropics compared with other travelers. A preexisting health problem did not predispose to a higher attack rate. Attack rates can be minimized by avoiding climatically extreme locations. This surveillance provides a focus of interest to the students, insight on minimizing avoidable health problems, evidences social responsibility by the Medical Faculty, and has the potential for expansion to other medical schools. Current Scottish medical school policies on HIV risk management would be strengthened by a more coordinated approach.

Adult↗