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At least 199 records · Page 11Linked to original sources
PubMed1990

[Methylation of Eco RI (GAm'ATTC) sequences of bacterial DNA].

The methylation of Eco RI (GAm6 ATTC) sequences of DNA of bacteria related to the main branches of their phylogenetic dendrogramme, was studied. It was found that methylation of Eco RI sites is observed in bacteria Caulobacter and in Thermus aquaticus. This finding can be substantiated by the resistance of these DNAs to Eco RI restrictase as well as by the fact that Bam HI fragments of these DNAs cloned within the composition of the vector plasmid pUC8 in E. coli cells contain GAATTC sites.

Bacteria↗
PubMedDate not supplied

[Effect of bacterial DNA gyrase inhibitors on the radiosensitivity of thymocytes and on the utilization of exogenous adenine in these cells].

It was established that the postirradiation changes in incorporation of 14C-adenine into acid-soluble and acid-insoluble fractions of thymocytes reflected cell death. When added after irradiation, nalidixic and oxolinic acids exerted a radioprotective action. The effect was absent after single washing of thymocytes incubated with these compounds for 60 min before or after irradiation. Both substances inhibited utilization of 14C-adenine and incorporation thereof into the acid-insoluble fraction of nonirradiated thymocytes and did not influence the viability of cells.

Adenine↗