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Stain for endamoebas.

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Coloring Agents↗
PubMed1978

Amebic peritonitis following rupture of an amebic liver abscess. Successful treatment of two patients.

We present two successfully treated cases of amebic peritonitis. Acute peritonitis secondary to intra-abdominal rupture of an amebic liver abscess is an infrequent but serious complication of invasive amebiasis. Its diagnosis should be considered in anyone with a suspected liver abscess, jaundice, or diarrhea in whom peritonitis develops. This diagnosis should be further suggested in the United States if the patient is a male and is of Mexican origin in areas where this racial group constitutes the majority of cases of amebic disease. Use of radioisotope liver scans and the demonstration of serum precipitins to Endamoeba histolytica may provide rapid evidence of invasive disease, although surgical intervention is often necessary to make a specific diagnosis. Emetine hydrochloride alone or followed by metronidazole combined with surgical drainage is the current treatment for amebic peritonitis.

Adult↗
PubMed2000

[Zoonotic parasitosis transmitted by dogs in the Chaco Salteño, Argentina].

Toxocara canis and Ancylostoma spp. are geohelmints that parasites dogs and can eventually affect humans, mainly children, causing visceral and cutaneous larva migrans respectively, constituting a serious public health problem. This study was carried out in two towns located in the xerophilous forest Chaco salteño where humans live closely with many animals, including dogs. Hematological values and anti-Toxocara canis antibodies, determined by ELISA in serum, were evaluated in 98 children from this area. Thirty-six children presented with eosinophilia of 10% or higher in peripheral blood. Twenty out of 98 (20.4%) children had antibodies against antigen from L2 larvae of Toxocara canis. A high percentage (55.6%) of the children with eosinophilia presented anti-Toxocara canis antibodies. Nine children had multiple serpiginous lesions typical of cutaneous larva migrans. Feces from dogs were collected in the area where children lived, in order to search for parasite contamination. Three different techniques of stool examination were employed and eggs were counted. Out of the 106 feces examined, parasites were found in 82 samples (77.4%). Ancylostoma spp eggs were found in 74 (69.8%) samples and eggs from Toxocara canis were found in 19 (17.2%). The average number of T. canis and Ancylostoma spp eggs/gr of feces, were 200 and 3,871 respectively. Giardia spp (14.5%), Trichuris vulpis (7.6%), Genus Endamoeba (2.8%) and Taenia spp (1.9%) were also identified in the stools. Sanitary control and health education in order to control these parasitoses are emphasized.

Animals↗
PubMed1961

A parasitological survey in three selected communities in Luapula Province, Northern Rhodesia.

The authors present the results of a parasitological survey conducted among children living in three rural and geographically separate communities selected for the work of the Health and Nutrition Scheme, Fort Rosebery, Northern Rhodesia. The survey included observations on Plasmodium spp. and other parasites of the blood, Endamoeba spp., and helminth and arthropod parasites. In addition, data on the distribution and taxonomy of local anophelines and fresh-water molluscs are given.As was expected, malaria, urinary bilharziasis and hookworm were the most important parasitic infections recorded, though the prevalence of each varied considerably in each community. Such local variation in prevalence of a particular parasitic infection should prove to be encouraging, rather than discouraging, to the initiation of control measures.The results of the present survey support the view, too frequently forgotten, that for an adequate understanding of the prevalence of different parasitic infections precise, local investigations are imperative; without such investigations, broad conclusions are seldom valid.

Child↗
PubMed1998

Evaluation of laboratory techniques for diagnosis of amoebiasis.

Evaluation of 82 cases of amoebiasis including 4 of liver abscess was done. Microscopy of 62 stool samples revealed trophozoites in 19 (30.6%) and cysts in 8 (12.9%). PVA preserved samples were stained by iron-hamatoxylin and trichrome. Twenty-nine samples (46.7%) were positive by culture on Endamoeba, NIH and Charcoal agar. Additional two culture positive cases were negative by direct microscopy. Three (75%) samples of liver abscess pus were culture positive. Of the sera screened for amoebic antibodies by ELISA, 13 (17.3%) were positive. Stool and blood of healthy controls and 25 gastroenteritis cases were similarly analysed. Of healthy controls one had a high ELISA titre.

Animals↗