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Mycoplasma gallisepticum infection in wild-type turkeys living in close contact with domestic fowl.

Mycoplasma gallisepticum was isolated from 2 wild-type turkeys (Meleagris gallopavo) and 1 domestic turkey living in close contact on a farm in Tehama County, California. Sinusitis was detected in 2 of 14 wild-type turkeys and in 1 of 12 feral broad-breasted bronze turkeys, but in none of several chickens on the premises. The entire mixed flock was captured, sinus aspirates were collected from affected birds, and blood samples were obtained from all birds for serologic testing. Blood samples also were obtained from 10 domestic turkeys on adjacent premises from which breeding stock had been borrowed. The M gallisepticum isolated from sinus aspirates was typed and inoculated into susceptible chickens, resulting in airsacculitis. California wild turkeys with and without histories of exposure to domestic fowl and wild turkeys shipped into California from Texas for release were tested for antibodies to M gallisepticum, using the plate agglutination test. Evidence of M gallisepticum infection was not found in wild turkeys at any location other than the original premises.

Air Sacs↗

[Studies of the origin of Chinese domestic fowls].

A total of 539 bases fragment of mitochondrial DNA D-loop region of six domestic chicken breeds (30 individuals) were sequenced and compared to that of red junglefowl, grey junglefowl, green junglefowl and Lafayette's junglefowl issued in GenBank, and the phylogenetic trees for the chickens constructed based on the D-loop sequences. The results indicated that the four species of genus Gallus had great differences between each other, the G. g. domesticus was the next of kin to red junglefowl in Thailand and its adjacent regions, and near of kin to red junglefowl in Indonesian, Lafayette's junglefowl, grey junglefowl and green junglefowl one by one in proper order, suggesting that the red junglefowl in Thailand and its neighbour areas sufficed as the matriarchic ancestor of Chinese domestic fowls. It was also discovered that the two subspecies of Thailand i.e. G. g. gallus and G. g. spadiceus should belong to one subspecies because of their far lower differentiation compared to that among the domestic breeds.

Animals↗

Experimental eosinophilia in domestic fowls and ducks following horse serum stimulation.

Multiple intraperitoneal injections of horse serum given to domestic fowls at five different ages resulted in eosinophilia in 55% of adults and in 36% of the younger birds, the highest individual eosinophil level reached being 50% of the differential white cell count. The primary eosinophilic response in fowls was between injections 7 and 36 and there was a secondary response between injections 32 and 72. Horse serum injections produced an eosinophilia in 88% of adult ducks with a maximum individual level of 31% of the differential white cell count. In ducks, primary and secondary eosinophilic responses occurred at injections 13 and 28 respectively.

Age Factors↗

Effect of starvation and protein-feeding on blood amino acid compartmentation of domestic fowl hatchlings.

The amino acid concentrations in plasma and blood cells of 5-day old domestic fowl hatchlings that received either standard feeding, protein-feeding or were starved have been determined. The effects of 5-day starvation or protein feeding did not alter significantly the combined amino acid concentration of blood plasma, but decreased blood cell levels. The patterns of individual amino acid changes observed in starvation or protein-feeding were similar in both groups when compared with those of controls. However, starvation-induced effects were actually more marked than those observed in protein-fed animals. The patterns of change with starvation of individual amino acids in the hatchling blood compartments were very different from those observed in mammals subjected to short or medium-term starvation. The mechanisms controlling circulating amino acid concentrations act in both situations studied to maintain the plasma amino acid concentrations despite marked changes in the availability of 2-amino nitrogen energy to the animal; changes in blood amino acid compartmentation buffering plasma amino acid availability.

Amino Acids↗

Faecal measurements of oestradiol and testosterone allow the non-invasive estimation of plasma steroid concentrations in the domestic fowl.

1. Radioimmunoassays were validated for oestradiol and testosterone in faecal and plasma samples from domestic fowls. Faecal samples were prepared in phosphate buffer. A delay of up to 24 h between defaecation and the freezing of a dropping did not affect faecal steroid concentrations. 2. Differences in plasma concentrations of testosterone and oestradiol between cockerels and hens were clearly reflected in faecal hormone concentrations. Faecal concentrations of oestradiol were low in males and did not overlap with much higher concentrations in females, whereas there was some overlap between the sexes in faecal concentrations of testosterone. 3. The correlation coefficients (r2) between plasma and faecal steroid concentrations were 0.464 for testosterone and 0.852 for oestradiol (log-transformed data). The coefficients did not increase when the mean hormone concentrations for several droppings produced by each bird during a collection period were used. 4. Faecal steroid concentrations can be used as a measure of plasma steroid concentrations and hence of gonadal activity in chickens. The resolution of the faecal steroid method is less than if plasma measurements were used, but it offers a practical alternative to blood sampling that is non-invasive and does not require birds to be handled.

Animals↗

A radiographic study of mineralised tendons in the domestic fowl.

1. The occurrence of mineralised tendons in the wing and pelvic limb of the domestic fowl was studied radiographically in adults and in growing birds from hatching to 182 d. 2. Mineralised tendons were found to occur in all the adults in the dorsal and plantar tarsometatarsus and variably in the caudal tibiotarsus, cranial metacarpus and caudal antebrachium. In the cranial tibiotarsus a mineralised tendon was detected in two cockerels only. 3. Mineralisation was detected from 91 d onwards, at first in tendons in the plantar tarsometatarsus and cranial metacarpus and later in the dorsal tarsometatarsus. The mineralised tendons found in adults at other sites were not found during the growing period studied.

Animals↗

Assay of sperm quality in the domestic fowl by MTT reduction.

1. An assay which measures the capacity of spermatozoa from the domestic fowl to reduce the colourless tetrazolium dye MTT to its highly coloured purple formazan pigment has been developed and characterised. 2. The assay has several advantages over a previous tetrazolium-based sperm quality assay: it functions without the unstable phenazine methosulphate and toxic cyanide reagents and, following the reductive reaction, the sperm suspension is solubilised to produce optically clear solutions without centrifugation. 3. For samples of semen from individual males, MTT-reduction is strongly correlated with INT-reduction, which has been previously shown to be a useful predictor of sperm fertilising ability.

Animals↗

Sperm mobility: A primary determinant of fertility in the domestic fowl (Gallus domesticus).

Previous research demonstrated that sperm mobility is a quantitative trait of the domestic fowl. The trait is quantified by measuring the absorbance of an Accudenz solution after overlay with a sperm suspension and brief incubation at body temperature. In the present work, average and high sperm mobility phenotypes (n = 30 males per phenotype) were selected from a base population. Differences were found between sperm oxygen consumption (p < 0.0001), acylcarnitine content (p < 0.05), linear velocity (p < 0.001), and straightness (p < 0.001), a trajectory variable measured with the Hobson SpermTracker. Oxygen consumption and stearoylcarnitine content of sperm from the high-mobility phenotype were twice those observed with sperm from average males, implying a pivotal role for mitochondria. On the basis of these results, a graded relationship was predicted between fertility and sperm mobility. Males (n = 48) were chosen at random from another base population, sperm mobility was measured per male, and each ejaculate was used to inseminate 8-12 hens (8 x 10(7) viable sperm per hen). When fertility was plotted as a function of sperm mobility, data points approximated a skewed logistic function. The hypothesis that vaginal immunoglobulins constitute an immunological barrier to sperm transport was tested and rejected. Therefore, we concluded that sperm mobility is a primary determinant of fertility in the fowl.

Animals↗

[Fine structure of the trigeminal nerve nucleus of the domestic fowl].

The trigeminal nerve nuclei are examined light- and electron-microscopically in the adult domestic fowl. The nucleus sensibilis principalis nervi trigemini is formed by scarce, medium-sized, round-to-ovoid polygonal neurons. The Nissl bodies are concentrated around the nucleus and consist of short cisterns of the rough endoplasmic reticulum densely bordered with ribosomes. The nucleus tractus spinalis nervi trigemini extends to the first segments of the cervical cord. The rostral part of the nucleus is characterized by medium-sized polygonal neurons. Their cell bodies are densely packed with coarse Nissl bodies. Small multiforme cell types with large nuclei frequently showing two nucleoli predominate in the caudal part. The motorical main portion, nucleus motorius nervi trigemini consists of medium-sized as well as great polygonal neurons. The accessory portion, nucleus motorius dorsalis nervi trigemini, consists of medium-sized polygonal neurons. Both nuclei show the typical motoneuron cytomorphology. In the neuropil, the axodendritic synapses can be differentiated into five types. Occasionally, densely packed glial lamellae and giant mitochondria occur.

Animals↗

Influence of fasting, glucose and insulin on the levels of growth hormone and prolactin in the plasma of the domestic fowl (Gallus domesticus).

The concentrations of both GH and prolactin in the circulation of the domestic fowl have been determined after various treatments known to affect carbohydrate metabolism. Fasting decreased the level of glucose, stimulated the secretion of GH and inhibited the secretion of prolactin. Administration of insulin significantly depressed the level of GH in the plasma of normal or fasted birds and also in chickens which had received simultaneous injections of glucose or 2-deoxy-D-glucose. No consistent effect of insulin on the secretion of prolactin was observed. Hyperglycaemia subsequent to administration of glucose had no effect on the levels of either GH or prolactin. Glucagon-induced hyperglycaemia suppressed the level of GH in the plasma and stimulated that of prolactin.

Animals↗

Comparison of various anesthetic regimens in the domestic fowl.

Three anesthetic agents (equithesin, metomidate, and ketamine) combined with diazepam were tested in the domestic fowl. Laparotomy and thoractomy were possible with the equithesin/diazepam combination, but only laparotomy was possible with the metomidate/diazepam combination. The combination of ketamine/diazepam did not result in the depth of anesthesia required for surgical procedures. Repeated blood pressure and heart rate measurements were recorded by use of a modified noninvasive Doppler technique. Equithesin/diazepam was our combination of choice for long-duration surgical anesthesia. The depth of anesthesia could be modulated by increasing the dose of diazepam. Metomidate/diazepam was useful when short-term anesthesia was required. In contrast to equithesin/diazepam, the metomidate/diazepam combination provided unstable anesthesia of varying depth and duration. Adverse reactions with metomidate indicated caution when using this drug in chickens; the drug also caused marked bradycardia. Ketamine/diazepam combination cannot be recommended as an anesthetic agent for use in chickens. The combination may be useful for minor surgical procedures or treatment, but not for experimental procedures that involve major surgery. Diazepam alone had a slight tranquilizing effect.

Anesthesia, General↗

Broiler pulmonary hypertension syndrome. II. The direct measurement of right ventricular and pulmonary artery pressures in the closed chest domestic fowl.

A technique for the catheterisation of the right ventricle and pulmonary artery of the domestic fowl is described. Mean peak systolic, minimum diastolic and mean pressures measured in the right ventricle and pulmonary artery of a group of clinically normal broilers housed at a medium altitude of 1,300 m were 24.3; 8.1; 17.8; 25.0; 22.1 and 22.3 mm Hg respectively. The pulmonary artery pressures are different from those reported elsewhere using the open chest technique. These differences are possibly due to both the effect of opening the thoracic cavity and the hypoxic pressor effect at this altitude.

Animals↗

Ultrastructural localisation of alkaline phosphatase in the testis of domestic fowl.

The ultrastructural distribution of alkaline phosphatase has been investigated in the testis of the domestic fowl. Enzyme activity was observed in the germinal epithelium and in the peritubular tissue. In both situations the activity was located in the plasma membranes. The activity in the germinal epithelium was marked basally in the germinal cells and in the Sertoli cells. There was a gradual reduction and an ultimate disappearance of enzyme activity towards the luminal region. The presence of enzyme in the germinal epithelium is in contrast to results of similar studies in some mammals. The peritubular localisation is comparable to that of mammals and confirms light microscope observations reported for the fowl testis.

Alkaline Phosphatase↗

Histology and immunology of Peyer's patches in the domestic fowl (Gallus domesticus).

Peyer's patches (PP) were identified in the small intestine of the domestic fowl by their thickened villi, flattened epithelium which lacked goblet cells and by the accumulations of lymphocytes in the form of encapsulated germinal centres (GC) and diffuse lymphoid tissue (DLT). Particles of orally administered carbon were seen in GC and DLT, in macrophages and within epithelial cells (M cells). Faults in the PP epithelium, which was positive for alkaline phosphatase, allowed the extrusion of lymphocytes into the intestinal lumen. Immunofluorescence detected more lymphoid cells with cytoplasmic IgG than IgA or IgM. Specific antibody production was seen in cells of GC ad DLT in PP from fowls that had received multiple injections of horse serum.

Animals↗

Preliminary investigation on the submicroscopical structure of blood capillaries of the pineal body in the embryonic and adult domestic fowl.

The morphological changes of the blood network capillaries in the pineal gland of the domestic fowl during embryonic development and in the first post-hatching year have been analyzed. Our preliminary results indicate that the first intrinsic vessels, showing an immature aspect, appear among the epithelial vesicles on 7th-8th incubation day (i.d.), when the pinealoblasts are actively proliferating; then, they drastically increase in number (10th i.d.), but they begin to undergo significant maturative changes only on the 19th i.d., in parallel with the unfolding of the pinealocytes differentiation. Finally they reach the definitive set-up within the first post-hatching year, while the pineal is acquiring its full morphological and functional maturity.

Aging↗

Effects of prostaglandins E1, E2 and F2 alpha and polyphloretin phosphate of domestic fowl.

The predominant effect of PGE1, E2, and F2 alpha on systemic arterial pressure of domestic fowl is hypotension. PGE1 and E2 are approximately equiactive and PGF2 alpha is about 10 times less active than E1 and E2, PGE1 and E2 produce sudden hypotension with gradual recovery while F2 alpha produces fall in blood pressure with both slow onset and recovery. Polyphloretin phosphate itself produces depressor responses in chickens and antagonizes the depressor responses to the prostaglandins dose-dependently.

Acetylcholine↗

Taste sensitivity in the embryo of the domestic fowl.

Two experiments were carried out to investigate the sense of taste in embryos of the domestic fowl. In the first, four taste substances; NaCl, HCl, glucose and SOA were diluted with distilled water and the response was compared with that to distilled water alone. No significant effects of taste were found. In the second experiment five taste substances: HCl, fructose, NaCl, KCl and quinine were diluted with fluids normally imbibed by the embryo: amniotic and/or allantoic fluid taken from other eggs. These solutions and also distilled water were compared with egg-fluid alone. A highly significant effect of the five solutions was found showing that the taste system becomes functional before the time of hatching. Distilled water produced on an unexpectedly large response in the embryo; possible reasons for this are discussed.

Animals↗

Oestrogen and progesterone receptors and their relationship to histological grades of epithelial tumours of the magnum region of the oviduct of the domestic fowl.

Tumours of the proprial tubular glands of the magnum region of the oviduct of the domestic fowl (Gallus domesticus) were found to contain receptors for oestrogen and progesterone. The distribution of the receptors in the cytosol and nucleus of the tumour cells was similar to that of the normal magnum under oestrogen stimulation. Receptor content showed a positive correlation with increasing clinical stage and histological grade, i.e., malignancy and undifferentiation of the tumours. These tumours are unusual for hormonally responsive tumours in that their receptor mechanism remains intact with loss of differentiation.

Animals↗