[Histochemical methods of study of phosphomonoesterases with chromogenic substrates; new technic for staining histological sections].
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For attaching histological sections to slides a new adhesive medium consisting of rubber silicon is described. Using this substance the damage to histological sections caused by various techniques and enzyme digestion (floating) may be eliminated. This method can be applied both to paraffin and frozen sections.
Authors worked out a new method for the histological demonstration of chloride ions by modifying old techniques. Unfixed, fresh tissue pieces are treated with silver nitrat, then embedded in paraffin. Chloride ions present in the tissue react with silvernitrate, thus forming silver chloride. Silver salts of other anions present in the tissue can be eliminated with weak solution of nitric acid, thus silver precipitate formed by reduction of silver chloride indicate only the localization of chloride ions. This method seems to be suitable for the demonstration of chlorid ions in the parietal cells of the stomach and for the lokalization of reabsorbed chloride ions in the renal tubules.
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Technical progress in sonographic equipment providing routinely high resolution images makes it appropriate that some criteria of normality have to be reviewed. In this paper normal sonographic images of the kidney are correlated with anatomy and histology. The effect of aging upon normal anatomical relations and histologic findings is analysed. Special attention is focussed on particular anatomical details as far as they can explain the particular topography or sonographic appearance of some pathologies.
Histological analysis of 91 roots from 80 freshly extracted teeth from patients with known calendar age and life-history parameters revealed that the validity of a chronological age-at-death diagnosis based on the count of incremental lines in human dental cementum is essentially dependent from two parameters: method of analysis, and pathological state of the investigated tooth. In this paper, we want to contribute to the establishment of a methodological standard procedure by comparison of our own results with otherwise published research designs. We conclude that as long as the necessary prerequisites are met, the quantitative analysis of cementum annulli will lead to a rather precise chronological age-at-death diagnosis. In addition, a qualitative analysis permits the ontogenetic dating of certain individual life-history parameters (e.g. previous pregnancies).
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