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PubMed1993

Problems in formulating method-insensitive proficiency testing materials.

Significant problems exist in formulating method-insensitive proficiency materials. Many steps are required in processing human plasma, and difficult choices are involved in the selection of appropriate materials to be added to the processed plasma. Additionally, analytes may vary widely in their recovery from method to method. To enhance understanding of the procedures and problems involved in the development and manufacture of proficiency materials, a number of constituents and their method-specific recoveries are reviewed.

Bias↗
PubMedDate not supplied

Linguistic background and test material considerations in assessing sentence identification ability in English- and Spanish-English-speaking adolescents.

This study investigated the influence of linguistic background differences on sentence identification by groups of English- and Spanish-English-speaking adolescents. Subjects were required to identify recorded meaningful and nonmeaningful (synthetic) sentences presented in a white noise background using a closed message set-response format. The results indicate that linguistic background variables significantly influence sentence identification ability and that these variables are not adequately controlled for by a closed message set. A significant difference in the ability to identify meaningful and nonmeaningful sentences was revealed. Contrary to previous indications, synthetic sentence identification appeared to be contingent upon key work or phrase recognition.

Adolescent↗
PubMed2005

Inverse parameter fitting of biological tissues: a response surface approach.

In this paper, we present the application of a semi-global inverse method for determining material parameters of biological tissues. The approach is based on the successive response surface method, and is illustrated by fitting constitutive parameters to two nonlinear anisotropic constitutive equations, one for aortic sinus and aortic wall, the other for aortic valve tissue. Material test data for the aortic sinus consisted of two independent orthogonal uniaxial tests. Material test data for the aortic valve was obtained from a dynamic inflation test. In each case, a numerical simulation of the experiment was performed and predictions were compared to the real data. For the uniaxial test simulation, the experimental targets were force at a measured displacement. For the inflation test, the experimental targets were the three-dimensional coordinates of material markers at a given pressure. For both sets of tissues, predictions with converged parameters showed excellent agreement with the data, and we found that the method was able to consistently identify model parameters. We believe the method will find wide application in biomedical material characterization and in diagnostic imaging.

Algorithms↗
PubMed2000

Assessment of acute cyto- and genotoxicity of corrosion eluates obtained from orthodontic materials using monolayer cultures of immortalized human gingival keratinocytes.

Whilst a patient is undergoing orthodontic treatment, dental appliances based on non-precious metals or titanium remain in the oral cavity for up to several years. Throughout this period the appliance is in either direct or indirect contact with the oral mucosa. To investigate the possibility of cell damage occurring as a result of appliance corrosion, monolayer cultures of immortalized human gingival keratinocytes were assessed for acute cyto- and genotoxicity using the hexosaminidase assay and the Comet assay respectively. The materials tested included 1. a nickel-free wire, 2. a UK-1 bond, 3. nickel-free as well as nickel-containing brackets with and without color signature and 4. a titanium expansion screw. Each of the test materials was corroded in a solution consisting of equal amounts of lactic acid and sodium chloride (0.1 M) for 1, 3, 7 and 14 days. The cell cultures were then exposed to eluates exhibiting the highest ion concentrations. None of the eluates was found to exhibit acute cytotoxicity, regardless of the type of test system used. Qualitative assessment using neutral red dye for live cells and either trypan blue or propidium iodide to disclose dead cells failed to reveal any significant increase in cell damage when exposed cells were compared to control cultures. Unrestricted cell vitality was confirmed by quantifying viable cells through measurement of hexosaminidase enzyme activity. Furthermore, assessment of genotoxicity revealed no apparent DNA damage to immortalized gingival keratinocytes following exposure to the test eluates. Because the materials tested in this study were corroded using the exacting methods normally applied to precious metals or gold-containing alloys, the lack of either acute cyto- or genotoxic effects following exposure to the test eluates indicates that the materials tested exert no adverse effects on cells similar to those of the target tissue exposed to the materials in situ.

Cell Culture Techniques↗
PubMed1999

Microwave disinfection of denture base materials colonized with Candida albicans.

STATEMENT OF PROBLEM: Infection of denture materials with Candida albicans is common and contributes to denture stomatitis. PURPOSE: This 3-phase investigation examined: (1) the efficacy of microwave irradiation against C albicans colonized on 3 soft denture liners and 1 heat-polymerized denture base resin, and (2) the effect of this irradiation on the hardness of the materials tested. MATERIAL AND METHODS: In phase 1, an experimental protocol was developed. Sterilized specimens from 2 denture base soft liners and 1 heat-polymerized acrylic resin denture base material (n = 45 each) were inoculated with C albicans. Two thirds of the specimens were irradiated in a 60 Hz microwave oven for 5 minutes (dry). C albicans growth was then assessed with streaked blood agar plates and thioglycollate broth. One third of the specimens were not irradiated and served as controls. Pretest and posttest Shore A hardness values were obtained and compared. For phase 2, 15 specimens from each material group were subjected to irradiation (while immersed in water) for 5 minutes; and, 15 from each material were subjected to 10- and 15-minute irradiation (dry), with subsequent sterility and change in hardness assessments completed as described in phase 1. In phase 3, 15 specimens from each material group were subjected to repeated 5-minute irradiation cycles (while immersed in water), and changes in hardness were examined. RESULTS: Only the 5-minute irradiated specimens immersed in water were effectively sterilized, as verified by the thioglycollate assay. The effect of repeated 5-minute irradiation cycles resulted in a significant change in hardness of the PermaSoft specimens. CONCLUSIONS: Five-minute irradiation, while immersed in water, killed all C albicans present on the materials tested; and, repeated 5-minute irradiation significantly affected the hardness of only the PermaSoft material.

Acrylic Resins↗
PubMed2002

Enzymatic responses of human deciduous pulpal fibroblasts to dental restorative materials.

The purpose of this study was to evaluate the responses of succinic dehydrogenase (SDH) and alkaline phosphatase (ALP) activities of human deciduous teeth pulpal fibroblasts (HDPF) to dental restorative materials. Tested materials included Z100 (3M), Dyract (Dentsply), FujiII (GC), and FujiIILC (GC). IRM (Dentsply) and culture medium (MD) alone were used as positive and negative controls, respectively. Specimens 6 mm (diameter) x 3 mm were prepared in accordance with manufacturers' instructions. For light-cured materials, specimens were light cured for 40 s on both sides under a celluloid strip. For chemical-cured materials, specimens were allowed to set at room temperature for 15 min. The specimens were immersed in 1 mL of culture medium without serum for 24 h at room temperature. The extracts were filtered through 0.22-mm filters. HDPF (10,000 cells/well) was incubated with 100 microL of extract and 20 % FBS in a 96-well plate for 24 h in a 37 degrees, 5 % CO(2) incubator. Six wells per material were prepared. Optical density (OD) of SDH and ALP of HDPF were measured by a spectrophotometer. The means were analyzed by ANOVA and then a Duncan Test. The ranking of OD of SDH was IRM < FujiIILC < FujiII = Z100 < Dyract < MD (p < 0.05). The ranking of OD of ALP was IRM < Z100 = Dyract < FujiII < FujiIILC < MD (p < 0.05). The result showed that all of the tested restorative materials were cytotoxic to human deciduous pulpal fibroblasts. The cytotoxicity of resin-modified glass ionomer cements (FujiIILC) was stronger than that of traditional glass ionomer cements (FujiII) and composite resin (Z100), and that of compomer (Dyract) was the weakest. On the contrary, ALP activities of resin-modified glass ionomer cements (FujiIILC) and composite resin (Z100) were higher than those of traditional glass ionomer cements (FujiII), while those of compomer (Dyract) were the lowest. It is concluded that, in this study, FujiIILC was the most cytotoxic material and the least inhibitive of ALP activities, Dyract was the weakest cytotoxic material and had the highest inhibition of ALP activities. The rankings of the MTT assay and the ALP assay were not consistent.

Alkaline Phosphatase↗
PubMedDate not supplied

Wetting properties of saliva substitutes on acrylic resin.

The good wetting of the acrylic resin by saliva substitutes is of clinical importance in xerostomic patients. This study evaluated the wetting properties of different artificial saliva formulations that were mucin-based, carboxymethylcellulose-based, and concentrated ion-based on poly(methyl methacrylate) denture base resin, and compared these properties with natural saliva. The wetting properties of the test materials were examined by contact angle measurements. Ninety-six samples that measured 30 x 30 x 3 mm were examined. The wetting properties of mucin-containing and carboxymethylcellulose-containing substitutes on poly(methyl methacrylate) were significantly better than those of human saliva. Mucin-containing artificial salivas had the best wetting properties on the acrylic resin for the materials tested.

Acrylic Resins↗
PubMed2003

A new austenitic stainless steel with negligible nickel content: an in vitro and in vivo comparative investigation.

New nickel (Ni)-reduced stainless-steel metals have recently been developed to avoid sensitivity to Ni. In the present study, an austenitic Ni-reduced SSt named P558 (P558, Böhler, Milan, Italy) was studied in vitro on primary osteoblasts and in vivo after bone implantation in the sheep tibia, and was compared to ISO 5832-9 SSt (SSt) and Ti6Al4V. Cells were cultured directly on P558 and Ti6Al4V. Cells cultured on polystyrene were used as controls. Osteoblast proliferation, viability and synthetic activity were evaluated at 72 h by assaying WST1, alkaline phosphatase activity (ALP), nitric oxide, pro-collagen I (PICP), osteocalcin (OC), transforming growth factor-beta1 (TGFbeta-1) and interleukin-6 (IL-6) after 1.25(OH)2D3 stimulation. Under general anaesthesia, four sheep were submitted for bilateral tibial implantation of P558, SSt and Ti6Al4V rods. In vitro results demonstrated that the effect of P558 on osteoblast viability, PICP, TGF beta-1, tumor necrosis factor-alpha production did not significantly differ from that exerted by Ti6Al4V and controls. Furthermore, P558 enhanced osteoblast differentiation, as confirmed by ALP and OC levels, and reduced IL-6 production. At 26 weeks, the bone-to-implant contact was higher in P558 than in SSt (28%, p<0.005) and Ti6Al4V (4%, p<0.05), and was higher in Ti6Al4V than in SSt (22%, p<0.005). The tested materials did not affect bone microhardness in pre-existing host bone as evidenced by the measurements taken at 1000 microm from the bone-biomaterial interface (F=1.89, ns). At the bone-biomaterial interface the lowest HV value was found for SSt, whereas no differences in HV were observed between materials (F=1.55, ns). The current findings demonstrate P558 biocompatibility both in vitro and in vivo, and osteointegration processes are shown to be significantly improved by P558 as compared to the other materials tested.

Alloys↗
PubMed1994

Mechanical behavior of glass ionomer cements affected by long-term storage in water.

OBJECTIVES: The early strength of glass ionomer cements was investigated previously (Cattani et al., 1993). Using the same set of glass ionomer cements, this study was designed to determine if mechanical property degradation occurred after an extended period of storage in water. METHODS: In the present study, the effect of aging in water on the compressive, tensile and flexural strengths of twelve commercial glass ionomer cements was evaluated at different time intervals after the beginning of the setting reaction. The aging periods varied from 24 h to 12 mon. RESULTS: The results show that the mechanical properties of the tested materials are in some cases affected by long-term aging in water. The evolution of strength follows distinct patterns of change. It has been established that the glass ionomer cements are neither characterized by a continuous increase nor a continuous decrease in strength. Most of the materials tested maintained a constant strength. However, several of them first display an improvement of the mechanical strength followed by a reduction. In this case, the 24h and the 12 mon strengths are usually similar. SIGNIFICANCE: The aging mechanisms of glass ionomers are complex. Strengthening probably results from additional crosslinking and build-up of a silica gel phase, whereas weakening may result from erosion and plasticizing effect of water.

Acrylic Resins↗