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The effects of intracanal medicaments, fillers, and sealers on the attachment of human gingival fibroblasts to an exposed dentin surface free of a smear layer.

To date there has been very little research into the possible effects of endodontic therapy on regeneration of the lost periodontal attachment. The objective of this study was to examine the effects of endodontic medicaments on fibroblast attachment to dentin surfaces free of a smear layer. Pulp chambers of extracted third molars were filled with one of the following medicaments: gutta-percha with Roth's zinc oxide and eugenol-based sealer, warm gutta-percha with sealer, warm gutta-percha without sealer, calcium hydroxide, formocresol, cotton pellet, or left empty. A predetermined dentin surface area was then inoculated with human gingival fibroblasts at a concentration of 2 x 10(4) cells per ml. The cells were allowed to adhere to the dentin surface for either 4 or 24 hours, then cell attachment was quantified using a methyl-tetrazolium assay. The data were analyzed using a Kruskal-Wallis one-way analysis of variance and Dunn's multiple comparison test. It was determined that fibroblast attachment was significantly reduced when exposed to formocresol or warm gutta-percha without sealer at both the 4 and 24 hour interval (P < or = 0.05). This suggests that the use of formocresol or warm gutta-percha without sealer in a root canal may impede periodontal wound healing and regeneration.

Analysis of Variance↗

Notes on the uses of psychoanalysis for biography.

It is the dual aim of this presentation to explore the scope and to define the limits of what psychoanalysis can offer to the field of biography. In pursuit of these goals emphasis is placed upon the influence of the writer's emotional attachment to his subject, which, despite intensive psychoanalytic training and experience, may compromise his objectivity and contaminate his narrative. In addition, attention is drawn to psychological issues in the life of the creative artist which may play a decisive role both in determining the quality of his work and in stimulating its production.

Biographies as Topic↗

Mothers' mental representations and their relationship to mother-infant attachment.

The association between the constructs of mental representation posited by object relations theory and attachment theory was studied through an assessment of adolescent mothers' mental representations and their infants' security of attachment. Forty-two pregnant adolescents were given the Adult Attachment Interview (George, Kaplan, & Main, 1985) to assess their modes of attachment, and the Krohn Object Representation Scale for Dreams (Krohn & Mayman, 1974) to assess object-representations. When the 42 infants were 15 months old, they were videotaped in the Strange Situation (Ainsworth, Blehar, Waters, & Wall, 1978) with their mothers. Subjects were primarily low-income African-American and Hispanic mothers and infants. As measured by the significant association between classifications on the Adult Attachment Interview and the Krohn scale (p less than .001), the adolescents' attachment and object-representations were highly related. Both attachment and object relations were also significantly related to infant attachment (p less than .001 and p less than .01, respectively). Results suggest that the concepts of mental representation proposed by object relations theory and attachment theory overlap and that mothers' mental representations have heuristic value in predicting mother-infant attachment.

Adolescent↗

Continuity and change in the measurement of infant attachment: comment on Fraley and Spieker (2003).

R.C. Fraley and S.J. Spieker (2003) refocused attention on the important question of continuous versus categorical measurement of infant attachment. This commentary begins with a brief response to Fraley and Spieker's position. Next, it highlights the usefulness of the categorical approach by reviewing some of the major advances in the field that have been fostered by a categorical approach. These include the identification of the disorganized attachment group, the development of the concept of conditional behavioral strategies, the creation of systems for coding attachment beyond infancy, and a greater understanding of cross-generational associations. The author then makes several propositions for future research that are necessary for clarifying how best to measure infant attachment.

Emotions↗

Suicide and infantile fixations.

Infantile fixations upon primary parental figures and relationships is one of several factors central to the suicidal situation. The association of infantile fixations to suicidal behavior relates not only to their presence or degree but to their traumatic quality and to the occurrence of patterns of relationship with others who are similarly fixated. Other significant features of such infantile fixations are their rigid and repetitive character. Case illustrations highlight both the infantile fixation phenomenon and efforts to change by forming new or different attachments, and the conflict between the two. Lastly, implications for psychotherapy include the needs to reduce the anxiety and self hatred associated with infantile ties; to provide a temporary regression as a first step towards transformation of fixation and mastery; and to accept, rather than to attack, the suicidal patient's infantile attachments and patterns.

Adult↗

Reflective capacity and its significance to the attachment concept of the self.

Attachment writing about the self systematically regards the working model of the self as a social product. At the same time, reflective self-capacity is regarded as a particularly salient aspect of the individual's current state of mind with respect to attachment. Additionally, evidence has accumulated that some individuals have been able to create a coherent working model of the self despite a history of negative attachment experiences. This paper proposes that in these circumstances it is necessary to conceive of a private self through which the history of attachment experiences may be creatively transformed.

Humans↗

Mechanisms and significance of reduced activity and responsiveness in resting frog tadpoles.

Hatchling Xenopus laevis tadpoles spend most of their time attached to objects or the water surface by mucus secreted by a gland on the head. While attached, swimming activity and responsiveness to swim-initiating stimuli are reduced over long periods of time. We have investigated the mechanisms and significance of this apparent long-term inhibition. In behavioural experiments we show, firstly, that innervation of the cement gland and GABA(A)-mediated inhibition are necessary for attachment to reduce responsiveness, and secondly, that denervation of the cement gland increases tadpole activity and increases their predation by damselfly nymphs (Zygoptera). To investigate the neuronal pathway from the cement gland to GABA(A) inhibition, we have devised an immobilized, inverted tadpole preparation where a weight attached to the mucus simulates the force as it hangs. Simulated attachment reduces responsiveness and spontaneous fictive swimming activity. We have recorded the activity and responses of trigeminal neurons innervating the cement gland. They are spontaneously active and simulating attachment results in a sustained increase in this activity. We propose that hanging from a mucus strand increases firing in cement gland afferents. This leads to tonic GABA inhibition that reduces tadpole activity and responses, and leads to fewer attacks by predators.

Animals↗