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At least 199 records · Page 11Linked to original sources

PubMed2002

Evidence for apoptosis correlated with mortality in the giant black tiger shrimp Penaeus monodon infected with yellow head virus.

Histological, cytochemical and ultrastructural changes in giant black tiger shrimp Penaeus monodon were investigated at various time intervals after injection with yellow head virus (YHV). Hemocytes, lymphoid organs (LO) and gills were the main focus of the study. After injection with YHV, onset of mortality varied from 36 h onward. By normal hematoxylin and eosin staining, the 3 tissues showed clear and increasing prevalence of nuclear condensation, pyknosis and karyorrhexis from approximately 36 h post-injection (p.i.) until death, although pathology was evident in the LO as early as 12 h p.i. in some shrimp. By nuclear DNA staining with 4',6-diamidino-2-phenylindole (DAPI) and by specific labeling of 3'-OH ends of nuclear DNA using a technique called terminal deoxynucleotidyl transferase-mediated deoxy-UTP nick-end labeling (TUNEL), cells of the 3 tissues showed evidence of chromatin condensation and DNA fragmentation, respectively. Both are generally considered to be characteristic of apoptosis. In addition to TUNEL labeling, evidence for DNA fragmentation was supported by the appearance of approximately 200 base pair DNA ladders at approximately 48 h p.i. in hemocytes of YHV-infected but not uninfected shrimp. Transmission electron microscopy (TEM) of LO tissue revealed features of apoptosis in tissues of YHV-infected shrimp only. These included marginated, condensed and fragmented chromatin without concurrent cytoplasmic damage. Histological, cytochemical, ultrastructural and biochemical data were consistent with the hypothesis that widespread and progressive apoptosis occurred in susceptible shrimp infected with YHV. Although no specific tests were carried out to determine whether this purported apoptosis was the cause of mortality, moribund shrimp had extensive deterioration of vital tissues such as the hemolymph, gills, heart and LO, suggesting that many essential bodily functions had been severely compromised. This probably resulted in the gross signs of lethargy and weakness seen, and it is reasonable to suggest that further, progressive deterioration could have led to the collapse of vital functions followed by death.

Animals↗

PubMed2002

Clearing mechanisms of Vibrio vulnificus biotype I in the black tiger shrimp Penaeus monodon.

Vibrio species' infections are a common sequelae to environmental stress or other disease processes in shrimp, but the mechanism by which the shrimp eliminate the bacteria is poorly understood. In this study, the penetration, fate and the clearing of V. vulnificus were investigated in Penaeus monodon. A bacterial disease isolate from a shrimp farm was identified as V. vulnificus biotype I. Polyclonal antiserum was raised in rabbits against the bacterium and the specificity was verified by ELISA and immunoblot against a range of Vibrio spp. and other gram-negative bacteria. The bacteria were then administered to P. monodon juveniles by injection, immersion and oral intubation. An indirect immunoperoxidase technique was employed in a time course study to follow the bacteria and bacterial antigens in the tissue of the shrimp. Bacteria were cleared by a common route, regardless of the method of administration. Observations in immersion challenge were similar to a combination of those for oral and injection challenges. With immersion, bacteria entered the shrimp through damaged cuticle or via insertion points of cuticular setae. Shortly after entry, whole bacterial cells were observed in the haemolymph and connective tissue. They were either phagocytosed by haemocytes, or broken down outside host cells. Haemocytes containing bacterial cells or antigens (HCB) were observed in the connective tissue and haemolymph. HCB accumulated around the hepatopancreas, midgut, midgut-caecum, gills, heart and lymphoid organ. Free bacterial antigens also accumulated in the heart and lymphoid organ. Bacteria entering through the mouth by oral intubation or immersion were broken down so that only soluble or very fine particles entered the hepatopancreas. Bacterial antigens passed through the hepatopancreas into the haemolymph. Antigens were initially observed in the haemolymph sinuses and subsequently accumulated in the heart and lymphoid organ. Bacterial antigens were released from the shrimp, initially through the gills and subsequently through hepatopancreatic B-cells, branchial podocytes and sub-cuticular podocytes.

Administration, Oral↗

PubMed2005

Four viral proteins of white spot syndrome virus (WSSV) that attach to shrimp cell membranes.

White spot syndrome virus (WSSV) is a major shrimp pathogen that has a widespread negative affect on shrimp production in Asia and the Americas. It is known that WSSV infects shrimp cells through viral attachment proteins (VAP) that bind with shrimp cell receptors. However, the identity of both WSSV VAP and shrimp cell receptors remains unclear. We used digoxigenin (DIG)-labeled shrimp hemocyte and gill cell membranes to bind to WSSV proteins immobilized on nitrocellulose membranes, and 4 putative WSSV VAP (37 kDa, 39 kDa and 2 above 97 kDa) were identified. Mass spectrometric analysis identified the 37 kDa putative VAP as the product of WSSV gene VP281.

Amino Acid Sequence↗

PubMed2002

Carriage of Vibrio species by shrimps harvested from the coastal waters of South West Cameroon.

OBJECTIVES: To determine the prevalence of Vibrio spp in unprocessed shrimps and their susceptibility to antibiotics. DESIGN: A prospective study of Vibrio spp associated with shrimps harvested from the coastal waters of South West Cameroon. SETTING: A laboratory based study at the Department of Life Sciences, University of Buea. Two hundred and thirty six shrimps harvested from the coastal towns of Limbe and Tiko, Cameroon, were examined for the prevalence of Vibrio spp using standard microbiologic procedures. The antibiotic sensitivity of isolates was determined using the Kirby-Bauer disc diffusion technique. RESULTS: Of the 236 shrimps examined, 73 (30.9%) were contaminated with Vibrio spp. Further, a total of 125 Vibrio strains were isolated from the contaminated shrimps. Of this number, 33 (26.4%) were V. cholerae, 55 (44%) V. parahaemolyticus, 34 (27.2%) V. alginolyticus and three (2.4%) V. vulnificus. Antibiotic susceptibility generally ranged from 68.8% for polymyxin B to 99.2% for gentamycin. Multiple resistant strains were noted, especially with V. parahaemolyticus and V. alginolyticus CONCLUSION: Shrimps maintain a reservoir of potential Vibrio spp in the coastal area of South West Cameroon. This finding is of epidemiologic and clinical significance.

Animals↗

PubMed1999

Extension of shelf life of whole and peeled shrimp with organic acid salts and bifidobacteria.

Microbiological and sensory characteristics of treated whole and peeled shrimp from the east coast of Saudi Arabia were evaluated. Shrimp samples were treated with organic acid salts with or without Bifidobacterium breve culture and stored in ice. Peeling alone extended the microbiological shelf life by 4 days. Treatment of whole shrimp with sodium acetate alone or potassium sorbate with bifidobacteria prolonged the microbiological shelf life by 3 days and increased the microbial generation time from 12.8 h (control) to 30.1 h or 31.4 h, respectively. The microbiological and sensory shelf life of peeled shrimp treated with sodium acetate was more than 17 days. Sodium acetate extended the microbial lag phase and lengthened the generation time (38.7 h compared to 15.8 h for the control). Micrococci and coryneforms were the predominant microorganisms in whole shrimp during storage. Treatment with sodium acetate maintained better sensory characteristics for peeled shrimp than potassium sorbate combined with bifidobacteria.

Animals↗

PubMed2000

Effects of gamma radiation on the conformational and antigenic properties of a heat-stable major allergen in brown shrimp.

This study was performed to evaluate the application of food irradiation technology as a method for reducing shrimp allergy without adverse effects. Shrimp heat-stable protein (HSP) was isolated and gamma irradiated at 0, 1, 3, 5, 7, or 10 kGy in the condition of solution (1 mg/ml), and fresh shrimp was also irradiated. Conformational change of irradiated HSP was monitored by means of spectrometric measures, enzyme-linked immunosorbent assay with mouse monoclonal antibody, or human patients' sera and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The ability of the immunoglobulin E of patients allergic to shrimp to bind to irradiated HSP was dose dependently reduced. The amount of intact HSP in an irradiated solution was reduced by gamma irradiation, depending on the dose. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis showed that the main band disappeared and the traces induced from coagulation appeared at a higher molecular weight zone. The binding ability of immunoglobulin E to allergens in the extracts from irradiated shrimp decreased, depending on the dose. The results provide a new method so that food irradiation technology can be applied to reduce allergenicity of shrimp.

Allergens↗

PubMed1999

Facilitated geranylgeranylation of shrimp ras-encoded p25 fusion protein by the binding with guanosine diphosphate.

A cDNA was isolated from the shrimp Penaeus japonicus by homology cloning. Similar to the mammalian Ras proteins, this shrimp hepatopancreas cDNA encodes a 187-residue polypeptide whose predicted amino acid sequence shares 85% homology with mammalian KB-Ras proteins and demonstrates identity in the guanine nucleotide binding domains. Expression of the cDNA of shrimp in Escherichia coli yielded a 25-kDa polypeptide with positive reactivity toward the monoclonal antibodies against Ras of mammals. As judged by nitrocellulose filtration assay, the specific GTP binding activity of ras-encoded p25 fusion protein was approximately 30,000 units/mg of protein, whereas that of GDP was 5,000 units/mg of protein. In other words, the GTP bound form of ras-encoded p25 fusion protein prevails. Fluorography analysis demonstrated that the prenylation of both shrimp Ras-GDP and shrimp Ras-GTP by protein geranylgeranyltransferase I of shrimp Penaeus japonicus exceeded that of nucleotide-free form of Ras by 10-fold and four-fold, respectively. That is, the protein geranylgeranyl transferase I prefers to react with ras-encoded p25 fusion protein in the GDP bound form.

Amino Acid Sequence↗

PubMed2004

Environmental impact of aquaculture-sedimentation and nutrient loadings from shrimp culture of the southeast coastal region of the Bay of Bengal.

Nutrient loadings were measured for surface seawater and bottom sediments of semi-intensive and improved extensive shrimp culture pond, adjacent estuary, and fallow land in the south-east coastal region of Bangladesh during August, 2000-January, 2001 to evaluate the impact of shrimp culture. The mean levels of nutrients found in the pond surface water were 108.780 mg/L for CaCO3, 0.526 mg/L for NH4+ -N, 3.075 wt% for organic carbon, 7.00 mg/L for PO4-P, 5.57 mg/L for NO3-N, and 7.33 mg/L for chlorophyll-a. The maximum mean value of H2S (0.232 mg/L) was found in estuarine water. Nutrients loading were found to be decreased with distance from the shrimp farm discharge unit in estuarine water. The mean level of organic matter, total nitrogen, and organic carbon were found in higher concentrations in sediments of cultured pond compared to bottom soil of adjacent fallow land at the same elevation. Extractable Ca values were found in higher concentration (550.33 ppt) in adjacent fallow land, as the shrimps for molting in shrimp ponds use extractable Ca. The relation between seawater H2S value and sediment pH (r = - 0.94); sediment organic carbon and sediment pH values (r = -0.76), sediment total nitrogen and sediment pH (r = -0.74) were found to be highly negatively correlated. Whereas the relation between seawater H2S value and sediment total nitrogen (r = 0.92), water NH4+ -N and sediment pH (r = 0.66) were found to be positively correlated. The results revealed that load of nutrients at eutrophic level in estuarine water, and decrease of soil pH; leading to acid sulphate soil formation indicates a negative impact of shrimp culture.

Animals↗

PubMed1991

Large mitochondrial DNA differences between morphologically similar Penaeid shrimp.

Sequencing of mtDNA fragments amplified with the polymerase chain reaction is used to examine genetic differences between species of Penaeid shrimp. The data show surprisingly high genetic differences among morphologically and ecologically similar species. Differences at silent sites between subgenera of shrimp are larger than between families of mammals. Between genera, Penaeid shrimp are more divergent than some orders of mammals. Because taxonomic differences are defined on morphological grounds, these results imply that the rates of molecular versus morphological evolution in shrimp are different than in mammals. Perhaps mtDNA evolution is accelerated in shrimp, or perhaps stabilizing selection over a long time period has reduced rates of morphological change. For Penaeid shrimp, morphological similarity can mask large genetic differences.

Amino Acid Sequence↗

PubMed1990

Influence of tadpole shrimp, Triops longicaudatus (Notostraca:Triopsidae), stocking rate on Culex tarsalis development in experimental field microcosms.

The effectiveness of 5 tadpole shrimp (TPS) stocking rates to reduce cohorts of Culex tarsalis was studied in field microcosms (0.81-m2). Larval/pupal abundance in microcosms containing greater than 5 TPS/m2 was significantly (P less than 0.05) lower than that of controls lacking tadpole shrimp due to predation. Adult mosquito abundances captured in emergence units above the microcosms stocked with tadpole shrimp were significantly (P less than 0.05) lower than that of controls at rates greater than or equal to 10 TPS/m2. Tadpole shrimp growth during 17 days of this study was inversely proportional to their stocking rate, and a linear relationship between size and stocking rate was plotted. Tadpole shrimp stocking rates also influenced rate of mosquito development, causing significantly (P less than 0.05) shorter periods for 50% emergence where shrimp were present when compared with that of controls. Adult male Cx. tarsalis emerged significantly earlier then females in microcosms stocked at 5 TPS/m2, while no significant (P greater than 0.05) differences were detected between the sexes at the remaining predator stocking rates.

Animals↗

PubMed1989

[Fluoride contents in tea and Sakura-shrimp in relation to other inorganic constituents].

The purpose of this study is to determine the fluoride contents of shrimp and of tea samples obtained from Shizuoka prefecture which is famous for tea products area and fishery market and to examine the relations between fluoride and other mineral contents, such as calcium, magunesium and phosphorus. Fluoride contents in tea produced in this area have been reported by Matsuura and Kokubu and other investigators, but more recent data are scarce. Samples were taken from a kind of shrimp known as Sakura-shrimp and from 4 kinds of commercial tea (coarse tea, 2 kinds of green tea and refined green tea). After having been dried and powdered, 1g of each sample was reduced to ashes at 550 degrees C for 10 hours with Ca(OH)2 as a fluoride fixative. Fluoride was distilled from each ash sample at about 140 degrees C with HClO4, and about 200 ml of distillate was collected from each sample. Fluoride contents were determined by means of ion-specific electrode. Calcium and magnesium contents were determined by atomic absorption spectrophotometry, and phosphorus contents were determined by Chen, Toribara and Warner's method. Total fluoride contents of the samples were as follows: 61.73ppm in shrimp, 180.16ppm in coarse tea, 72.62ppm and 89.02ppm in the 2 kinds of green tea, and 71.11ppm in refined green tea. More than 99% of the total fluoride was obtained from 150ml distillate of each sample. Calcium contents were extremely high in shrimp (21,822ppm) and 2,106-2,693ppm in tea samples. Magnesium contents were highest in shrimp (3,088ppm) and lowest in coarse tea (1,333ppm).(ABSTRACT TRUNCATED AT 250 WORDS)

Animals↗

PubMedDate not supplied

Homogeneous sample preparation of raw shrimp using dry ice.

Sample homogeneity is critical to accurate and reproducible analysis of trace residues in foods. A method of uniform sample preparation using dry ice is described for shrimp. Other sample preparation techniques for raw shrimp produce nonhomogeneous samples. Sample homogeneity was determined through analysis of chloramphenicol added to intact tiger or white shrimp prior to sample preparation. Simulated chloramphenicol residue levels were 50, 15, 10, and 5 ppb. No significant differences were noted when analyses of shrimp inoculated with chlor-amphenicol prior to sample preparation with dry ice were compared with analyses of shrimp spiked after grinding with dry ice. Grinding shrimp with dry ice produced samples with homogeneous chloramphenicol residues. This technique should be applicable to other tissues and vegetable products.

Animals↗

PubMed2026

Microdroplet-based high-throughput screening for antagonistic bacteria targeting penaeid shrimp pathogenic Vibrio harveyi.

Antagonistic bacteria that suppress the growth of specific bacteria have attracted attention as an antibiotics-independent strategy for infectious disease control in aquaculture. Microfluidics-based water-in-oil droplets (microdroplets) enable high-throughput screening of antagonistic bacteria in the field of medicine or agriculture. However, the use of this screening system in aquaculture has not yet been reported. In particular, penaeid shrimp aquaculture, one of the largest sectors of global aquaculture, has a strong demand for alternative disease control strategies because vaccination is ineffective. Here, we demonstrated a proof-of-concept study of microdroplet-based high-throughput screening system for antagonistic bacteria targeting penaeid shrimp pathogenic Vibrio harveyi. Using this screening system, we successfully isolated 18 bacterial candidates with potential growth-inhibitory activity, representing three genera (Pseudoalteromonas, Shewanella, and Tenacibaculum), from the bacterial community of kuruma shrimp Penaeus japonicus rearing water. 16S rRNA gene-based bacterial community analysis revealed that these isolates included several low-abundance, rare taxa. Although these isolates showed no inhibitory activity on agar plates, one out of four tested strains showed a trend toward improved survival during co-infection tests using kuruma shrimp. Overall, our study highlights both the potential and limitation of microdroplet-based antagonistic bacterial screening to accelerate the development of biological control strategies in shrimp aquaculture.

Animals↗

PubMed1999

Ultracytochemical location of Na(+)/K(+)-atpase activity and effect of high salinity acclimation in gill and renal epithelia of the freshwater shrimp Macrobrachium olfersii (Crustacea, Decapoda).

Accumulation sites of lead phosphate reaction product consequent to Na(+)/K(+)-ATPase activity in gill and renal epithelia of the freshwater shrimp Macrobrachium olfersii were located ultracytochemically by para-nitrophenyl-phosphate hydrolysis and lead precipitation, and quantified per unit membrane area and cytoplasmic volume. In shrimps in freshwater (<0.5 per thousand S, 20 mOsm/kg H(2)O, 0.7 mEq Na(+)/liter), numerous sites of electron-dense, Na(+)/K(+)-ATPase reaction product accumulation were demonstrated in the membrane invaginations of the mitochondria-rich, intralamellar septal cells (12.5 +/- 1.7 sites/microm(2) membrane, 179 +/- 22 sites/microm(3) cytoplasm, mean+/- SEM, N </= 7) and in the basal region of the medial renal tubules (19.8 +/- 1.8 sites/microm(2) membrane, 437 +/- 53 sites/microm(3) cytoplasm), but not in the pillar cells whose apical flanges form the primary interface with the external medium. A putative, ouabain-insensitive Na(+)- or H(+)-ATPase was found in the apical microvilli of the medial renal tubules (17.4 +/- 1.7 sites/microm(2) membrane, 629 +/- 101 sites/microm(3) cytoplasm). This restricted location of Na(+)/K(+)-ATPase activity within the gill epithelium suggests that during uptake, Na(+) moves across the apical pillar cell membrane, passes through specialized, basolateral coupling junctions into the septal cell cytoplasm and is pumped into the hemolymph via the Na(+)/K(+)-ATPase in the invagination membranes. In shrimps acclimated to a high-salinity medium (21 per thousand S, 630 mOsm/kg H(2)O, 280 mEq Na(+)/liter) for 2 and 5 days, the mean number of sites of para-nitrophenylphosphatase activity/microm(2) membrane and /microm(3) cytoplasm for both epithelia increases markedly by 83 and 163%, respectively. However, after 10 days acclimation, the number of sites declines dramatically, attaining values far below those for shrimps in freshwater. These acclimation-induced alterations in numerical density/microm(3) cytoplasm cannot be accounted for by corresponding changes in membrane surface density (microm(2) membrane/microm(3) cytoplasm) and reflect a real alteration in the number of Na(+)/K(+)-ATPase reaction product sites/unit membrane area. These data suggest that neither the gill nor the renal Na(+)/K(+)-ATPase systems function at maximal activity in shrimps in freshwater, possibly due to the low Na(+) concentration, and are initially stimulated by the increase in external ionic concentration. However, these powerful Na(+) transport systems respond to salt loading by a notable reduction in the number of hydrolysis sites, possibly through the incorporation of the Na(+)/K(+)-ATPase into isolated membrane vesicles in the basal invaginations of the medial renal tubules, together with ultrastructural alterations like the spatial isolation of the mitochondria by multiple membrane stacks in the intralamellar septal cells. J. Exp. Zool. 284:617-628, 1999.

Adaptation, Physiological↗

PubMed1993

Specific phosphorylation of membrane proteins of Mr 44,000 and Mr 32,000 by the autophosphorylated insulin receptor from the hepatopancreas of the shrimp Penaeus monodon (Crustacea: Decapoda).

The insulin receptor, purified from the hepatopancreas of the shrimp Penaeus monodon, is a hydrophobic heterodimer of subunits with relative masses (Mr) of 70,000 and 58,000, as estimated by FPLC on Superose 12 and SDS-PAGE. Only the subunit of Mr 70,000 was autophosphorylated after the addition of insulin. The autophosphorylation occurred specifically at Tyr residues, as demonstrated by the specific subsequent dephosphorylation by the phosphotyrosyl protein phosphatase from the hepatopancreas of the shrimp Penaeus monodon. Proteins of Mr 44,000 and Mr 32,000 on the plasma membrane from the hepatopancreas of the shrimp Panaeus monodon were phosphorylated by the autophosphorylated insulin receptor from the shrimp hepatopancreas, but not by that from the human placenta. The detergent, Triton X-100, caused noticeable enhancement of the autophosphorylation of both shrimp and human insulin receptors.

Amino Acid Sequence↗

PubMed2003

Morphology of the midgut trunk in the penaeid shrimp, Sicyonia ingentis, highlighting novel nuclear pore particles and fixed hemocytes.

The morphology of the midgut trunk (MGT) in the penaeid shrimp Sicyonia ingentis was examined by light and scanning and transmission electron microscopy. Although the function of the MGT is poorly understood, it is not involved with the digestion and absorption of nutrients, and it appears to be the surface of a shrimp least protected from penetration by potential pathogens. As described for other decapod crustaceans, the MGT in shrimp is composed of a simple columnar epithelium separated from a layer of connective tissue by a thick basal lamina. Beneath the basal lamina is a previously unreported layer of hemocytes, exclusively of the granulocyte variety, embedded in a matrix continuous with the basal lamina and extending into the connective tissue. This layer was observed in four other species of penaeid shrimp. Granulocytes in circulation can phagocytose and encapsulate foreign material and the granules contain antibacterial molecules, lysosomal enzymes, and prophenoloxidase. We suggest that the granulocytes associated with the basal lamina have matured at this site and are well positioned to fight potential pathogens that have penetrated the epithelial layer of the MGT. A second observation is the presence of clusters of cylinders bound to the nuclear pores of the epithelial cells. The possibility that these clusters are viruses, organelles, or abnormal organelles induced by disease or toxic materials is discussed. These unique particles were observed in S. ingentis but none of the other penaeid shrimp we examined.

Animals↗

PubMed2006

Analysis of novel angiotensin-I-converting enzyme inhibitory peptides from protease-hydrolyzed marine shrimp Acetes chinensis.

Acetes chinensis is an underutilized shrimp species thriving in the Bo Hai Gulf of China. In a previous study, we had used the protease from Bacillus sp. SM98011 to digest this kind of shrimp and found that the oligopeptide-enriched hydrolysate possessed antioxidant activity and high angiotensin I-converting enzyme (ACE) inhibitory activity with an IC50 value of 0.97 mg/ml. In this paper, by ultrafiltration, gel permeation chromatography and reversed-phase high-performance liquid chromatography (RP-HPLC), five peptides with high ACE inhibitory activity were purified from the shrimp hydrolysates and their sequences were identified by amino acid composition analysis and molecular weight (MW) analysis. Three of them, FCVLRP (a), IFVPAF (f) and KPPETV (j), were novel ACE inhibitory peptides. Their IC50 values were 12.3 microM, 3.4 microM and 24.1 microM, respectively, and their recoveries were 30 mg/100 g (solid basis of shrimp), 19 mg/100 g and 33 mg/100 g, respectively. Lineweaver-Burk plots for the three novel peptides showed that they are all competitive inhibitors. To test the ACE inhibitory activity of peptide a, f, j after they were digested by digestive enzymes in vivo, 12 derived peptides from FCVLRP and IFVPAF were synthesized based on their amino acid sequences and the cleavage sites of digestive enzymes. No digestive enzyme cleavage site was found in KPPETV. The IC50 values of the derived peptides were determined and the result showed that except for VPAF, FC and FCVL, the ACE inhibitory activity of the other nine derived peptides did not significantly change when compared with their original peptides. Surprisingly, five peptides had lower IC50 values than their original peptides, particularly for RP (IC50 value = 0.39 microM), which is about 30 times lower than its original peptide and almost the lowest IC50 value for ACE inhibitory peptides reported. Therefore, the novel peptides identified from A. chinensis hydrolysates probably still maintain a high ACE inhibitory activity even if they are digested in vivo. This is the first report about novel ACE inhibitory peptides from hydrolysates of marine shrimp A. chinensis. The novel peptides from hydrolysate of A. chinensis and some of their derived peptides with high ACE inhibitory activity probably have potential in the treatment of hypertension or in clinical nutrition.

Angiotensin-Converting Enzyme Inhibitors↗

PubMed1977

Characterization of some fish and shrimp spoiling bacteria.

The classification of some important groups of bacteria involved in fish and shrimp spoilage was studied. Trimethylamine is produced by Pseudomonas putrefaciens, a "non-defined" group resembling Ps. putrefaciens, Photobacterium spp. and some Moraxella-like bacteria. Hypoxanthine is produced by the same groups of bacteria except the last named and also by the "typical shrimp spoilers" (presumptive Alteromonas). Strong off-odours are produced on fresh fish by Ps. putrefaciens, dextrose-oxidative Pseudomonas spp. (Groups I and II according to Shewan, Hobbs and Hodgkiss, 1960), the above mentioned "non-defined" group and by only some of the "typical shrimp spoilers", whereas Moraxella-like bacteria and Photobacterium spp. failed to produce strong odours. Strong off-odours are produced on boiled shrimp by the "typical shrimp spoilers" (presumptive Altermonas). Ps. putrefaciens, the dextrose-oxidative Pseudomonas spp. and the "non-defined" group; Moraxella-like bacteria produced less offensive odours or none, nor did Photobacterium.

Alcaligenes↗