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At least 217 records · Page 12Linked to original sources
PubMed1983

[Choice of nutrient media for the laboratory diagnosis of Streptococcus group B].

The comparative study of a large assortment of liquid and solid culture media used for the cultivation of streptococci in laboratory practice in the USSR and abroad was carried out with the aim of selecting the optimal media for the laboratory diagnosis of group B. streptococci. Liquid media were tested with the use of 7 streptococcal reference strains, and some of these media, found to yield the best results, were selected for tests on clinical material. The use of liquid accumulation media was shown to permit the isolation of group B. streptococcal strains which could not be detected by the direct inoculation of clinical material into dishes with blood agar. The character of hemolysis induced by group B. streptococci in solid media with 5% of blood added was found to depend on the composition of the medium and the conditions of cultivation.

Cross Infection↗
PubMed2002

[Value of laboratory diagnosis in inflammatory rheumatologic diseases].

Laboratory investigations play an important part in the diagnostic procedures of inflammatory rheumatic diseases. Besides the classical humoral inflammatory parameters (erythrocyte sedimentation rate, C-reactive protein, protein electrophoresis), hematology, blood chemistry, infectious serology and synovial fluid analysis we want to focus in this review on the more detailed diagnostic by autoantibody testing as well as by specific molecularbiological procedures (PCR technique).

Autoantibodies↗
PubMed2000

Laboratory diagnosis of malaria.

Malaria particularly falciparum malaria is a major public health problem in India. Its correct and early diagnosis is very important for prompt treatment as a preventive and control measure. Microscopy is the traditional method for laboratory diagnosis of malaria, which is used widely. However, it is time consuming, needs expertism and the detection limit is 10-20 parasites/ml blood in thick film and 100 parasites/ml blood in thin film. Quantitative buffy coat technique (QBC) is highly sensitive method but expensive equipment is needed for this test. The serological methods involving antibody detection give information regarding exposure to malaria but do not differentiate between present and past infections. Genetic probes and PCR are highly sensitive methods but require expensive equipments. Parasite antigen detection tests are useful in field and PHC level for rapid diagnosis of P falciparum malaria.LDH based test for diagnosing malaria is sensitive but can not differentiate between species. For monitoring of drug resistance or follow-up of patients, methods which can quantify parasitaemia are needed. Simply microscopy is the best solution at present.

Clinical Laboratory Techniques↗
PubMed1992

Use of a direct enzyme-linked antiglobulin test for laboratory diagnosis of immune-mediated hemolytic anemia in dogs.

Detection of autoantibody, complement, or both bound to RBC is an essential requirement for unequivocal diagnosis of immune-mediated hemolytic anemia in dogs. An enzyme-linked antiglobulin test was adapted for laboratory diagnosis of this disease. The refinement and routine use of this assay have allowed further observation of the pathogenesis of the disease process. In particular, degree of hemolysis can be related to the degree of RBC sensitization associated with primary immune-mediated hemolytic anemia, and this correlation is highest for IgG autoantibody. Results indicate that autoantibody isotype might have an important role in the hemolytic process.

Anemia, Hemolytic, Autoimmune↗
PubMed1991

A dot-immunobinding assay for the laboratory diagnosis of tuberculous meningitis and its comparison with enzyme-linked immunosorbent assay.

In an attempt to establish an alternative to standard bacteriological methods in the laboratory diagnosis of tuberculous meningitis (TBM), a simple dot-immunobinding assay (Dot-Iba) was standardized to detect Mycobacterium tuberculosis antigen 5 and antimycobacterial antibody in cerebrospinal fluid (CSF) specimens of patients with TBM. Sensitivity and specificity of Dot-Iba was compared with conventional enzyme-linked immunosorbent assay (ELISA) and standard bacteriological techniques. The Dot-Iba showed excellent correlation with indirect ELISA for the detection of antimycobacterial antibody in CSF and showed 60% sensitivity and 100% specificity in culture-negative patients with TBM. However Dot-Iba was less sensitive for the detection of antigen 5 in CSFs and showed false negative results (60%) in culture-positive patients with TBM.

Antibodies, Bacterial↗
PubMed2004

Meta-analysis of the clinical and laboratory diagnosis of appendicitis.

BACKGROUND: The importance of specific elements in the clinical diagnosis of appendicitis is controversial. This review analyses the diagnostic value of elements of disease history, clinical findings and laboratory test results in suspected appendicitis. METHODS: A systematic Medline search was made of all published studies on the clinical and laboratory diagnosis of appendicitis in patients admitted to hospital with suspected disease. Meta-analyses of receiver-operator characteristic (ROC) areas, and positive and negative likelihood ratios, of 28 diagnostic variables described in 24 studies are presented. RESULTS: Inflammatory response variables (granulocyte count, proportion of polymorphonuclear blood cells, white blood cell count and C-reactive protein concentration), descriptors of peritoneal irritation (rebound and percussion tenderness, guarding and rigidity) and migration of pain were the strongest discriminators, with ROC areas of 0.78 to 0.68. The discriminatory power of the inflammatory variables was particularly strong for perforated appendicitis, with ROC areas of 0.85 to 0.87. Appendicitis was likely when two or more inflammatory variables were increased and unlikely when all were normal. CONCLUSION: Although all clinical and laboratory variables are weak discriminators individually, they achieve a high discriminatory power when combined. Laboratory examination of the inflammatory response, clinical descriptors of peritoneal irritation, and a history of migration of pain yield the most important diagnostic information and should be included in any diagnostic assessment.

Appendicitis↗
PubMed1993

Kennedy's disease: clinical presentation and laboratory diagnosis.

Kennedy's disease is a form of progressive spinal and bulbar muscular atrophy of adult onset. This paper describes a case of Kennedy's disease and discusses the laboratory diagnosis and the underlying genetic mechanism. Three other neurological diseases, Huntington's disease, myotonic dystrophy and fragile X syndrome, which have similar genetic defects, are also discussed.

Aged↗
PubMed1996

Laboratory diagnosis of insulinoma in the dog: a retrospective study and a new diagnostic procedure.

Simultaneous insulin and glucose measurements from a single sample are often insufficient for the laboratory diagnosis of insulinoma in the hypoglycaemic dog. Although the simple insulin (microU/litre): glucose (mmol/litre) ratio is best, when the selected threshold is set at 13.5, it is not always efficient to confirm or refute insulinoma. The fasting test consists of collecting four samples on a given day (no accurate time period is set between any two samples) from a fasting dog for simultaneous insulin and glucose measurements to detect at least one abnormal insulinaemia peak.

Animals↗
PubMed1984

Laboratory diagnosis of mycoplasma infections.

The relative efficiency of different media for the isolation of M. pneumoniae is discussed, with emphasis on the utility of SP-4 medium as a primary medium for isolation. Media available for isolation and identification of ureaplasmas are also described. Rapid methods of identification of isolated mycoplasmas are surveyed. The use and value of serologic procedures in the clinical laboratory are evaluated with particular reference to the complement fixation procedure and its value as compared to other methods. The possibilities for the development of direct antigen detection procedures for clinical use are discussed with particular reference to ELISA and other antigen capture methods. The problems that arise in susceptibility testing of isolates are raised, and a usable procedure is proposed both for minimal inhibitory concentration and minimal bacteriocidal concentration determination. A general procedure for the laboratory diagnosis of mycoplasmal infection that is adaptable to various laboratory circumstances and needs is proposed.

Anti-Bacterial Agents↗
PubMed2003

Laboratory diagnosis of invasive mycoses.

Rising numbers of immunocompromised patients have led to an ever-increasing population at risk of invasive fungal disease. Much has been achieved in the laboratory diagnosis of these infections, such as advances in blood culture systems, and the development of new biochemical, antigen detection assays, and molecular methodologies. More standardized susceptibility testing guidelines provide for better therapeutic interventions. In an era of economic cutbacks in health care, future challenges include the development of cost-effective and technically simplified systems, which provide early detection and identification of common and emerging fungal pathogens. It will, however, take some time to establish the clinical relevance of these new methodologies in different patient populations.

Culture Media↗
PubMed2006

Laboratory diagnosis and management challenges in the antiphospholipid syndrome.

The antiphospholipid syndrome (APS) is characterized by recurrent arterial and/or venous thrombosis and pregnancy morbidity manifested by early or late losses. Laboratory diagnosis ofAPS relies on the demonstration of a positive test for antiphospholipid antibodies (aPL). In clinical practice, the gold standard tests are those that detect anticardiolipin antibodies (aCL) and/or the lupus anticoagulant (LA). Although other specificities for aPL have been described their clinical utility and standardization has still to be established. Persistence of aPL positive tests must be demonstrated, and other causes and underlying factors considered. Although it is universally recognized that the routine screening tests (aCL and/or LA) might miss some cases, careful differential diagnosis and repeat testing are mandatory before the diagnosis of 'seronegative APS' can be made. Correct identification of patients with APS is important, because prophylactic anticoagulant therapy can prevent thrombosis from recurring, and treatment of affected women during pregnancy can improve fetal and maternal outcome.

Abortion, Habitual↗
PubMedDate not supplied

[Dot-blot analysis in laboratory diagnosis of hemorrhagic fever with renal syndrome].

A sensitive micro EIA utilizing antigen or antibody dotted onto nitrocellulose filters (Dot-ELISA, Dot-blot) has been developed for laboratory diagnosis of hemorrhagic fever with the renal syndrome (HFRS) by detecting the virus specific IgM and IgG in the sera of HFRS patients and the antigen in crude lung suspensions of wild rodents. Measurements of specific IgM and IgG in paired HFRS sera collected during the first month of the disease showed clear-cut seroconversion and 100% correlation with the results of immunofluorescent test. The results of antigen detection in lung suspensions of 605 wild rodents trapped in various regions of Russia were identical to standard ELISA results. This rapid and inexpensive test may be useful in early serologic diagnosis of HFRS and in field serological and epizootological studies.

Animals↗
PubMed1991

[Laboratory diagnosis of pneumonia caused by Mycoplasma pneumoniae].

The authors demonstrated the possibility to use the technique of counter current immunoelectrophoresis, enzyme immunoassay and passive hemagglutination tests with antibody erythrocytic diagnosticum for the rapid laboratory diagnosis of Mycoplasma infection. The rapid diagnosis of Mycoplasma pneumonias turned to be possible due to the detection of antigen and antibodies to M. pneumoniae in the circulation with the help of counter current immunoelectrophoresis.

Adult↗
PubMed1995

The laboratory diagnosis of pneumonia. The role of the community hospital pathologist.

Pneumonia is one of the most serious infections seen in community hospital practice, with virulent bacteria and viruses producing infections in the healthy host and a variety of opportunistic organisms capable of causing disease in the immunocompromised patient. Accurate laboratory diagnosis is extremely important for correct clinical management of pneumonia, and the community hospital pathologist can take an active role in daily review of respiratory tract specimens to optimize and coordinate this important laboratory testing. This article discusses strategies for improving sputum Gram stain interpretation and for the use of both routine and supplementary cultures in community-acquired pneumonias and outlines a comprehensive consultative approach for rapid and reliable pneumonia diagnosis in the compromised patient.

Biopsy↗
PubMed1984

Differential laboratory diagnosis of hypercalcemia.

It is proposed that this review will adopt the following format: establishment of hypercalcemia. This demands a discussion of the problem of normal ranges, the usage of either total calcium or ionized calcium in making this decision and where total calcium is used whether adjustment of this value for serum protein concentration should be used and if so, the formulae which have been cited to perform this. Having established hypercalcemia why is it necessary to differentiate this? This will involve reviewing those clinical situations in which differentiation of hypercalcemia has been attempted and will include an attempt to produce an up to date indication of conditions in which hypercalcemia has been described. When hypercalcemia has been established the laboratory tests which have been further used to discriminate will be divided into single tests such as N- or C- terminal parathormone, 1,25- dihydroxycholecalciferol, cyclic AMP; the combination tests which have been used including phosphate clearance, chloride vs. bicarbonate etc. proceeding to those groups which have used discriminant function to help in the decision making; dynamic testing will also be discussed particularly with reference to steroid suppression but will also include other known suppressants such as Mithramycin and Calcitonin. A final section will be included attempting to assess overall the present state of art in differentiating laboratory diagnosis of hypercalcemia and will also attempt to highlight those areas which appear to be most fruitful areas of progress in the future.

Calcium↗