Characterization of cross reacting isolates of foot & mouth disease virus.
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Foot-and-mouth disease (FMD) is the most contagious animal virus disease of cloven-hoofed livestock and requires reliable and accurate diagnosis for the implementation of measures to control effectively its spread. Routine diagnosis of FMD is carried out at the OIE/FAO World Reference Laboratory for Foot-and-Mouth Disease (WRL for FMD), Pirbright by the combined use of ELISA and virus isolation in cell culture supplemented by reverse transcription polymerase chain reaction (RT-PCR) methods. These techniques require skilled personnel and dedicated laboratory facilities which are expensive. The development of a rapid and simple test for the detection of FMD virus antigen using Clearview chromatographic strip test technology for field application is described. This device detected FMD viral antigen in nasal swabs, epithelial suspensions and probangs from clinical samples submitted from the field, from animals infected experimentally and in supernatant fluids resulting from their passage in cell culture. The test system was more sensitive than ELISA for the diagnosis of all seven serotypes of FMD virus in the epithelial suspensions and nasal swabs and had equivalent sensitivity to the ELISA for the detection of contemporary virus strains in cell culture supernatant fluids. The study demonstrated the potential for this device to confirm a clinical diagnosis at the site of a suspected FMD outbreak, thereby offering the possibility of implementing control procedures more rapidly. Such pen-side diagnosis would have particular benefits in FMD emergencies, relevance to FMD control programmes which operate in endemic regions of the world such as South East Asia and for increasing disease awareness in other areas where efforts to control disease may be difficult. In each circumstance the availability of a pen-side device for diagnosis would reduce the necessity for sending routine diagnostic samples to an FMD laboratory and thereby reduce the delay in diagnosis, which can in some areas be considerable.
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Fibromatosis of the plantar fascia is a much less common occurrence than that of the palmar fascia and it is rarer still in children. It is essential to differentiate this entity from fibrosarcoma. In spite of its high incidence of local recurrence and the histological presence of mitotic figures, conservative resection is the treatment of choice. Recurrence following dermofasciectomy and skin grafting is less common than simple excision of the nodules in the fascia. Amputation should never be the treatment.
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This article classifies various forms of arthritis which may affect the foot. Osteoarthritis of the foot usually involves the first metatarsophalangeal joint or the ankle, the latter usually being secondary to prior trauma. Secondary osteoarthritis of the foot is frequently due to Charcot destruction which is usually secondary to diabetic neuropathy. Seronegative spondyloarthropathies include ankylosing spondylitis and Reiter's syndrome. The three variants of psoriatic arthritis of the foot are also discussed.
Sire genetic evaluations for protein yield, productive life, and selected type traits from the US were correlated with sire evaluations for disease from Denmark and Sweden and were then adjusted to approximate genetic correlations. Disease categories from Denmark included reproductive diseases, foot and leg diseases, metabolic and digestive diseases, and all diseases other than mastitis. Genetic evaluations for Denmark were from separate analyses for each disease category using a multiple-trait sire model with first, second, and third lactations handled as multiple traits. Evaluations from Sweden for all diseases other than mastitis were from a single-trait sire model using only first lactations. In addition, Danish and Swedish genetic evaluations were regressed on US type evaluations to test for quadratic relationships. Relationships were based on 104 bulls with US and Danish evaluations (88 with US type) and 84 bulls with US and Swedish evaluations (83 with US type). Genetic correlations between US protein yield and diseases were unfavorable, but correlations were favorable between productive life and disease. Genetic correlations among US type and diseases were around zero, except for correlations with US dairy form (range -0.34 to -0.73). Genetic correlations calculated from residual correlations (adjusted for predicted transmitting abilities for milk) between productive life and diseases were favorable (range 0.29 to 0.51). Genetic correlations calculated from residual correlations (adjusted for predicted transmitting abilities for milk) between dairy form and diseases ranged from -0.10 to -0.53. Selection for increased productive life may reduce disease occurrences, but selection for higher dairy form scores will increase disease occurrences.
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