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At least 217 records · Page 12Linked to original sources

Residues of insecticides, fungicides, and herbicides on Ontario-grown vegetables, 1980-1985.

Between 1980 and 1985, 354 composite vegetable samples representing 9 vegetable commodities were collected from farm deliveries to the market place in Ontario, Canada. All samples were analyzed for insecticides, 275 for fungicides, and 135 for herbicides. The analyses included organochlorine, organophosphorus, synthetic pyrethroid, and N-methylcarbamate insecticides; dithiocarbamate, acylalanine, phthalimide, dicarboximide, and other fungicides; and, chlorophenoxy acid, chlorobenzoic acid, bipyridilium, phenylurea, carbamate, and other herbicides. The commodities tested included asparagus, beans, carrots, cauliflower, cucumbers, onions, potatoes, sweet corn, and tomatoes. In most samples, pesticide residues were below the detection limits (i.e., 0.005 to 0.1 mg/kg), and most of the positive findings were a fraction (i.e., less than 1 to 20%) of the maximum residue limit (MRL) permitted for each commodity under the Canadian Food and Drugs Act and Regulations. A small number of samples had residues that exceeded the MRL, and these involved aldicarb and linuron on potatoes and chlorobromuron on carrots.

Fungicides, Industrial↗

Multiresidue method for determining substituted urea herbicides in foods by liquid chromatography.

A method is described for determining substituted urea herbicides in foods. The residues are extracted from the product with methanol, and the food coextractives are removed by using solvent partitioning and Florisil column chromatography. The extract is analyzed using liquid chromatography with postcolumn photodegradation, chemical derivatization with orthophthalaldehyde, and spectrofluorometry. Recoveries were determined by spiking 8 different food products with 6 phenylureas--chlorbromuron, chloroxuron, diuron, fluometuron, linuron, and metobromuron--at 0.05 and 0.5 ppm. Three determinations were made at each level for each product. Average recovery at 0.05 ppm was 95% (with a standard deviation of 7.9%), and at 0.5 ppm, 98% (with a standard deviation of 6.9%).

Chromatography, Liquid↗

Herbicidal treatments for control of Cannabis sativa L.

In order to test herbicides for the destruction of illicit stands of cannabis (Cannabis sativa L.) a series of commercially available herbicides were sprayed on glasshouse-grown plants having 2 to 6 leaves. The following herbicides caused complete kill or severe injury to cannabis plants: (a) herbicides with root and foliage activity--ametryn, atrazine, metribuzin, prometryn, terbutryne, diuron, fluometuron, linuron, methabenzthiazuron, phenobenzuron, ethofumesate, karbutilate, methazole and oxadiazon; and (b) foliar-acting herbicides with brief or no soil persistence--amitrole, bentazon, 2,4-D, diquat + paraquat, glyphosate and phenmedipham. In field experiments herbicides of the latter group, and ioxynil, metribuzin, and a MSMA-cacodylate mixture, caused death or severe damage to young cannabis plants. Glyphosate, ioxynil and bentazon destroyed developed cannabis plants. In glasshouse and field experiments the following herbicides applied to young cannabis plants caused marked deformations of stems, leaves and/or inflorescences: barban, butralin, dalapon, difenzoquat, dinitramine, diphenamid, IPC, napropamide, penoxalin, triffuralin, and U-27267.

Cannabis↗

Herbicidal treatments for control of Papaver somniferum L.

Fifty-five commercially available herbicides were evaluated for possible use to destroy illicit opium poppy crops (Papaver somniferum). In the first stage, herbicides were sprayed on poppy plants grown in containers. The following compounds killed poppy plants: (a) herbicides with typical foliar activity--amitrole, bromoxynil, 2,4-D, glyphosate, ioxynil and paraquat; and (b) herbicides with root and foliar activity--the triazines ametryn, atrazine, metribuzin, prometryn, simazine and terbutryn; the substituted ureas benzthiazuron, chloroxuron, diuron, fluometuron, linuron, methabenzthiazuron, neburon and phenobenzuron; and the miscellaneous compounds karbutilate, methazole, oxadiazon and pyrazon. Severe but sublethal injury was caused by cycloate, EPTC, molinate, pobulate, cacodylate + MSMA, ethofumesate, perfluidone and phenmedipham. Abnormal development of vegetative or reproductive parts of the plant was induced by benefin, butralin, dinitramine, pendimethalin, trifluralin, diphenamid, napropamide, dalapon and propham. Efficient herbicides with negligible persistence in soil at the doses applied were evaluated on poppy plants in the field at various stages of growth. Small plants were severely injured by 2,4-D, killed rapidly by bromoxynil, ioxynil, paraquat (in mixture + diquat), and more slowly by glyphosate and metribuzin. The resistance to herbicides increased with the age of the poppy plant. Severe damage with partial kill of developed plants was obtained with bromoxynil, ioxynil, glyphosate, and paraquat + diquat; the last treatment produced the fastest effect.

Drug and Narcotic Control↗

High-pressure liquid chromatographic analysis of urea herbicides in foods.

A method is described for the direct analysis of the urea herbicides linuron, monuron, diuron, chlorbromuron, fluometuron, chloroxuron, and fenuron in cabbage, corn, potatoes, turnip, and wheat at 0.01-1.0 ppm. The samples are extracted with acetone; the filtrate is partitioned with hexane-methylene chloride (1+1) followed by 2 other extractions with methylene chloride. The organic phases are dried and concentrated for column chromatographic cleanup on 2% deactivated Florisil. The column fractions are evaporated just to dryness and redissolved in 10 ml isooctane for high-pressure liquid chromatography on a 25 cm silica gel (5 mum) column. Isopropanol in isooctane is the mobile phase, and compounds are measured by ultraviolet absorption at 254 nm. Recoveries are greater than 80% in most cases. These results are confirmed by alkylation with sodium hydride-methyl iodide in dimethyl sulfoxide to form the methyl products which are analyzed by the same chromatographic system or by gas-liquid chromatography.

Chromatography, High Pressure Liquid↗

Determination of phenylurea herbicide residues in vegetables by liquid chromatography after gel permeation chromatography and Florisil cartridge cleanup.

A liquid chromatographic method for quantitative determination of 9 phenylurea herbicide residues (metoxuron, metobromuron, monolinuron, chlortoluron, isoproturon, diuron, linuron, chloroxuron, and neburon) in potatoes, carrots, and mixed vegetables is described. Samples are extracted with acetone, partitioned with ethyl acetate-cyclohexane (50 + 50, v/v) and cleaned up by gel permeation chromatography with ethyl acetate-cyclohexane (50 + 50, v/v) as eluant. A small column (1 cm id) packed with Biobeads SX3 resin is used to reduce solvent consumption and analytical time. After solid-phase extraction on a Florisil cartridge, herbicide residues are successfully separated on a C18 column by gradient elution and determined by UV detection at 242 nm. Average recoveries of 9 compounds from different samples range from 70 to 98% at 0.010 and 0.100 mg/kg fortification levels. Quantitation limits are 0.010 mg/kg.

Chromatography, Gel↗

Electrically driven microseparation methods for pesticides and metabolites. II: on-line and off-line preconcentration of urea herbicides in capillary electrochromatography.

Capillary electrochromatography (CEC) was introduced to the separation of nine important urea herbicides using octadecyl-silica (ODS) capillary columns that were specially designed to allow the realization of a relatively strong electroosmotic flow (EOF) and, in turn, fast separations. The ODS stationary phase was intentionally prepared to have a low surface coverage in octadecyl ligands in order to ensure a strong EOF. This ODS stationary phase of low surface coverage exhibited the usual reversed-phase chromatographic behavior as was manifested by the linearity of plots of log kappa versus the percent organic modifier in the mobile phase. The nature of the organic modifier of the mobile phase influenced the order of elution as well as the separation efficiency of the nine urea herbicides. Mobile phases containing acetonitrile yielded higher separation efficiency (by a factor of 1.5) than methanol-containing mobile phases. This was attributed to the higher mass transfer resistances of the solute in and out of the pores in the presence of the more viscous methanol-containing mobile phases. Due to the relatively strong affinity of the urea herbicides to the ODS stationary phase, on-line preconcentration consisting of prolonged injections allowed the determination of 10(-5) M urea herbicide samples using a UV detector without sacrificing separation efficiency. This was further decreased to 10(-7) M when the prolonged injection was preceded by the injection of a plug of water. The plug of water (the more retentive mobile phase) brought about an enhanced accumulation of the dilute samples into a narrow band at the inlet of the CEC column. When this on-column sample enrichment approach was combined with an off-line sample preconcentration step, which consisted of a solid-phase extraction process, ultra dilute samples of 10(-10) M (0.1 ppb) could be detected.

Chromatography, High Pressure Liquid↗

Gas chromatography mass spectrometry of some thermally labile urea pesticides.

The application of gas chromatography/mass spectrometry to three thermally labile phenylurea pesticides is reported. Using alcohols as solvents the decomposition of the pesticides is followed by a reaction in which esters of N-(3,4-dichlorophenyl)carbamic acid are formed. As these reactions occur during the gas chromatographic analysis, it is the esters which are identified in the mass spectrometer. The methyl ester is itself a pesticide so an erroneous conclusion about the original pesticide could be reached when methanol is the solvent.

Diuron↗

[Studies on the embryotoxicity of monolinuron and buturon in NMRI-mice (author's transl)].

The acute LD50 in virginal NMRI-Mice was found to be 2528 mg/kg for monolinuron and 1791 mg/kg for buturon. Pregnant female mice of the NMRI strain were administered orally 25--1000 mg monolinuron/kg (I) and 100-400 mg buturon/kg (II) on days 6--15 of gestation and, during defined phases of fetal development (days 10--13 after conception), 500 mg I/kg and 350 mg II/kg. Following administration of 10 doses, an increase of postimplantative losses and clear retardation of development in the upper dose range from 100 mg I/kg and 300 mg II/kg as well as a dose-dependent increase of the rate of cleft palates could be observed. High doses of both substances given from day 6--15 of pregnancy produced minor numbers of wavy and fused ribs as well as hypoplasia of the upper jaw after application of monolinuron and exenteria and exencephaly after buturon. Administration of monolinuron between days 10 and 13 of gestation resulted a minor and that of buturon a clear increase of the number of cleft palates. To evaluate postnatal development 200 and 500 mg I/kg, and 200 and 300 mg II/kg were administered orally on days 6--15 of gestation. In the higher doses, both substances produced an increased mortality among the offspring up to 3 weeks after birth, and a clear increase of the rate of cleft palates.

Abnormalities, Drug-Induced↗

A comparative study on pesticide formulations for application in running waters.

Release rates into water of monolinuron, desmetryne, and carbofuran from three different types of formulations have been studied to control aquatic weeds in running waters. The migration of active ingredients (AI) from conventional wettable powder formulations was complete within 1 day, while the migration from ethylene-vinyl acetate copolymeric matrix (EVA) lasted significantly longer due to low diffusion coefficients of monomeric pesticides in polymeric matrices Do = 10(-8)-10(-12) (cm2 X sec-1). The herbicidal action could be extrapolated up to several years. Release duration of AI from calcium alginate beads lasted 2 weeks. Herbicidal effectiveness of terbutryne-EVA formulation has been investigated in a laboratory-scale simulated flow system device with duckweed plants (Lemna minor) as test organism. Growth inhibition of duckweed could be achieved at approx 20 ppb terbutryne in steady state.

Absorption↗

Fate of [14C]monolinuron in potatoes and soil under outdoor conditions.

[phenyl-14C]Monolinuron was applied (2.5 and 1.9 kg/ha) to the soil surface of an outdoor lysimeter in two successive years: then, potatoes were grown. Total recovery of 14C in soil, plants, and leached water was about 55% (of 14C applied) after the first growing period and about 43% after the second growing period. Radioactivity in soil contained 77.1% (based on total 14C recovered in soil) bound residues, 15% monolinuron, and the following conversion products: N-(4-hydroxyphenyl)-N'-methoxy-N'-methylurea, N-(4-chlorophenyl)-N'-methylurea. N-(4-chlorophenyl)methylcarbamate, N-(4-chlorophenyl)-N-methyl-methylcarbamate, and 4-chloroformanilide. The leachate contained 0.8% (based on total 14C recovered in leachate) N-(4-hydroxyphenyl)-N'-methoxy-N'-methylurea. Potato plants contained 0.106 mg/kg radioactive residues in peeled tubers after one growing period and 15.94 mg/kg in the tops; after two growing periods, peeled tubers contained 0.091 mg/kg and tops contained 18.87 mg/kg radioactive residues. These residues consisted of bound 14C (57.9% of total 14C recovered in plants), N-(4-hydroxyphenyl)-N'-methoxy-N'-methylurea, N-(4-chlorophenyl)-N'-methylurea, N-(4-chlorophenyl)methylcarbamate, N-(4-chlorophenyl)-N-methyl-methylcarbamate, and 4,4'-dichlorozobenzene.

Biodegradation, Environmental↗

[Biotransformation of monolinuron (N-(4-chlorophenyl)-N'-methyl-N'methoxyurea) in isolated perfused chicken liver].

The biotransformation of radioactively labelled monolinuron (N-(4-chloro[U-14C]phenyl)-N'-methyl-N'-methoxyurea) was studied in the isolated perfused liver of the chicken. After a 4-hr perfusion, 83.1% of the added radioactivity was recovered, 56.6% in the perfusion medium and 26.5% in the liver and bile. The fraction of radioactivity extractable from the perfusion medium into ethyl acetate amounted to 47.8% of the added dose. In addition to monolinuron, five breakdown products were identified in this extract, namely N-(4-chlorophenyl)-N'-hydroxymethyl-N'-methoxyurea, N-(4-chlorophenyl)-N'-methoxyurea, N-(4-chlorophenyl)-N'-methylurea, 4-chlorophenylurea and 4-chloroacetanilide. Of particular interest was the absence of arylhydroxylated monolinuron derivatives, since in monolinuron-metabolism studies in the laying hen 2-hydroxy-4-chlorophenylurea and 3-hydroxy-4-chlorophenylurea were both detected. This differing metabolism corresponds to earlier findings in the rat, in which arylhydroxylated breakdown products were detected only in in vivo studies and not in rat-liver perfusion. Possible reasons for the differing metabolism of monolinuron in vivo and in vitro are discussed.

Animals↗

Follow-up testing of rodent carcinogens not positive in the standard genotoxicity testing battery: IWGT workgroup report.

At the Plymouth Third International Workshop on Genotoxicity Testing in June 2002, a new expert group started a working process to provide guidance on a common strategy for genotoxicity testing beyond the current standard battery. The group identified amongst others "Follow-up testing of tumorigenic agents not positive in the standard genotoxicity test battery" as one subject for further consideration [L. Müller, D. Blakey, K.L. Dearfield, S. Galloway, P. Guzzie, M. Hayashi, P. Kasper, D. Kirkland, J.T. MacGregor, J.M. Parry, L. Schechtman, A. Smith, N. Tanaka, D. Tweats, H. Yamasaki, Strategy for genotoxicity testing and stratification of genotoxicity test results-report on initial activities of the IWGT Expert Group, Mutat. Res. 540 (2003) 177-181]. A workgroup devoted to this topic was formed and met on September 9-10, 2005, in San Francisco. This workgroup was devoted to the discussion of when it would be appropriate to conduct additional genetic toxicology studies, as well as what type of studies, if the initial standard battery of tests was negative, but tumor formation was observed in the rodent carcinogenicity assessment. The important role of the standard genetic toxicology testing to determine the mode of action (MOA) for carcinogenesis (genotoxic versus non-genotoxic) was discussed, but the limitations of the standard testing were also reviewed. The workgroup also acknowledged that the entire toxicological profile (e.g. structure-activity relationships, the nature of the tumor finding and metabolic profiles) of a compound needed to be taken into consideration before the conduct of any additional testing. As part of the meeting, case studies were discussed to understand the practical application of additional testing as well as to form a decision tree. Finally, suitable additional genetic toxicology assays to help determine the carcinogenic MOA or establish a weight of evidence (WOE) argument were discussed and formulated into a decision tree.

Acetamides↗