NTP Center for the Evaluation of Risks to Human Reproduction: phthalates expert panel report on the reproductive and developmental toxicity of di-isodecyl phthalate.
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Retained placenta, endometritis, cystic ovaries and low conception rates are often considered as interconnected conditions in cattle. Wide ranges in amounts of selenium and vitamin E supplements have been advised in late-pregnancy diets and, frequently, they can be synergistically beneficial. Animals at grass or with other green foods should be of adequate vitamin E status, but dietary selenium deficiencies are both more frequent and severe. Selenium status is important for super-ovulation in cattle and multiple births in ewes because of its importance in sperm transport and establishment of ova. However, the potential toxicity of selenium-enriched feed supplements restricts their use on a free-access basis. Giving sodium selenite or selenate by injection provides only a short-term response in the plasma. Barium selenate given in oil by injection provides a more durable response but has potential tissue residue problems. Compressed metallic selenium powder with iron powder in high-density reticulo-ruminal pellets gives sustained release, but uncertainties regarding possible surface coating and the variable effects of selenium particle size may require additional grinders to ensure prolonged release. Neither reticulo-ruminal pellets of such composition nor barium selenate by injection may be used worldwide because only sodium selenite and selenate have general regulatory approval. A sustained-release multi-trace element/vitamin rumen bolus system effective for several months has increased lambing percentages in ewes, and increased herd conception rates and reduced the spread of calving for herds of beef cattle.
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There are basically two major problems in the genesis of "cloned" gametes in mammals, which have not been addressed in the original debate article. There is no adequate discussion on the mechanisms providing for high fidelity of chromosome segregation in mitosis and meiosis, and for proper imprinting in construction of "reconstituted gametes". The original debate article is uncritical with respect to the currently insufficient database and the incomplete documentation of results.
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The orchid Ophrys sphegodes Miller is pollinated by sexually excited males of the solitary bee Andrena nigroaenea, which are lured to the flowers by visual cues and volatile semiochemicals. In O. sphegodes, visits by pollinators are rare. Because of this low frequency of pollination, one would expect the evolution of strategies that increase the chance that males will visit more than one flower on the same plant; this would increase the number of pollination events on a plant and therefore the number of seeds produced. Using gas chromatography-mass spectrometry (GC-MS) analyses, we identified more than 100 compounds in the odor bouquets of labellum extracts from O. sphegodes; 24 compounds were found to be biologically active in male olfactory receptors based on gas chromatography with electroantennographic detection (GC-EAD). Gas chromatography (GC) analyses of odors from individual flowers showed less intraspecific variation in the odor bouquets of the biologically active compounds as compared to nonactive compounds. This can be explained by a higher selective pressure on the pollinator-attracting communication signal. Furthermore, we found a characteristic variation in the GC-EAD active esters and aldehydes among flowers of different stem positions within an inflorescence and in the n-alkanes and n-alkenes among plants from different populations. In our behavioral field tests, we showed that male bees learn the odor bouquets of individual flowers during mating attempts and recognize them in later encounters. Bees thereby avoid trying to mate with flowers they have visited previously, but do not avoid other flowers either of a different or the same plant. By varying the relative proportions of saturated esters and aldehydes between flowers of different stem positions, we demonstrated that a plant may take advantage of the learning abilities of the pollinators and influence flower visitation behavior. Sixty-seven percent of the males that visited one flower in an inflorescence returned to visit a second flower of the same inflorescence. However, geitonogamy is prevented and the likelihood of cross-fertilization is enhanced by the time required for the pollinium deposited on the pollinator to complete its bending movement, which is necessary for pollination to occur. Cross-fertilization is furthermore enhanced by the high degree of odor variation between plants. This variation minimizes learned avoidance of the flowers and increases the likelihood that a given pollinator would visit several to many different plants within a population.
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