[Role of Russian scientists in discovery and development of the bacteriologic method of control of harmful rodents].
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To look for possible developmental effects in the offspring of jojoba meal-treated Wistar rats, and to distinguish between the effects of reduced food intake and the specific developmental effects of jojoba meal itself, mated female rats were divided into three groups of 20 rats. They received during gestation: (a) normal rodent food (control group); (b) normal rodent food supplemented with 3% defatted jojoba meal (jojoba group); or (c) normal rodent food pair-fed with the jojoba group (pair-fed group). The jojoba meal group showed approximately 30% inhibition of food intake. Ten rats from each group were killed on gestation day 21. Compared to the control group, foetal body weight was reduced in both the jojoba and pair-fed groups, with a greater reduction in the jojoba group. Skeletal ossification was retarded to the same extent in both the jojoba and pair-fed groups. The other 10 rats from each group were left to produce litters. Compared with controls, the body weight of the pups was lower in both the jojoba and pair-fed groups; the reduction was slightly greater in the jojoba group, but this difference disappeared after 1 week. The offspring showed no other abnormalities and reproduced normally. We conclude that, at the dose used, the retardation in foetal skeletal ossification, induced by jojoba meal supplementation during gestation, is due to food intake inhibition. Moreover, the lower birth weight of the young of jojoba-treated dams compared with the pair-fed group is merely due to a lower body weight gain during gestation.
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An analytical method is developed for defining the residual quantities of rodenticide "Brodifacoum" (talon) used in the control of harmful field rodents and especially for species Microtus. The method is based on extracting the pesticide with chloroform and making use of spectrophotometry in the ultraviolet field in intervals 340-240 nm. The second derivative of the absorption is utilized as well as the band with maximum 288 nm and minimum 228 nm. The peak height in this interval is in proportion to the concentration of the brodifacoum. When analysing residual waters, reservoirs, and soil the elimination of impeding substances is performed by thin-layer chromatography. The "cis" and "trans" form of brodifacoum is jointly determined by spectrophotometry. At thin-layer chromatography processing the two forms are separated. Sensitivity of the method 0.5 microgram/cm3 (in chloroform solution). Reproduction (as coefficient of variation) 5%. A thin-layer chromatographic method is recommended for semiquantitative determination of brodifacoum.
The present study was undertaken to determine the effects of alpha-chlorohydrin at a dosage of 50, 100 or 150 mg/kg body weight and were killed 2 or 10 days later. alpha-chlorohydrin at employed dose levels induced selective damage to the germinal epithelium which seemed to be confined to one "cycle" only. No permanent epididymal lesions were observed in any of the treated bandicoots even following a toxic dose of the drug. Administration of this compound at a dose level of 50 or 100 mg/kg also had no effect on the weight of the testis or sex accessory organs or fructose levels in coagulating glands when compared to controls. These data indicate that the alpha-chlorohydrin possibly acts directly on the testis without influencing the normal endocrine status of the animal.
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