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At least 217 records · Page 12Linked to original sources
PubMed1981

Density and apparent location of the sodium pump in frog sartorius muscle.

The binding of the cardiosteroid 3H-ouabain to frog skeletal muscle was determined by studying the kinetics of its uptake and release. The amount of ouabain bound as a function of drug concentration in the external medium follows a hyperbolic relationship with a maximum binding (Bmax) of the order of 2500 molecules per square micrometer of surface membrane and an affinity constant (K) of 2.2 X 10(-7)M. The data do not suggest a drug-receptor (Na pump site) relation other than one-to-one. Ouabain molecules are released from whole muscle into ouabain-free media very slowly. The release is a single exponential function of time (tau approximately equal to 25 hr). When re-binding is prevented by the presence of unlabeled ouabain in the external medium, the loss of labeled ouabain is increased (tau approximately equal to 15 hr). Increasing [K+]O from 2.5 to 10 mM slows the time course of binding without any significant change in binding capacity of the muscle fibers. Experiments on detubulated muscles indicate that the density of pump sites is considerably higher in the surface than in the T-tubular membrane. These findings agree with the report by Narahara et al. [Narahara, H.T., Vogrin, V.G., Green, J.D., Kent, R.A., Gould, M.K. (1979) Biochim. Biophys. Acta 552:247] on the distribution of (Na+ + K+)- ATPase among different cell membrane fractions from frog skeletal muscle.

Animals↗
PubMed2003

Water-lily sign on MR imaging of primary intramuscular hydatidosis of sartorius muscle.

Primary hydatidosis of muscle is very rare and can cause a variety of diagnostic problems, especially in the absence of typical radiologic findings. This is the report of a case of primary intramuscular hydatid cyst in a 35-year-old woman, who presented with a 1-year history of a painless lump in the distal left thigh. The authors document the pathognomonic water-lily sign that has not previously been reported in a case of intramuscular hydatid disease.

Adult↗