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Measurement of rate constants for quenching singlet oxygen with a Cypridina luciferin analog (2-methyl-6-[p-methoxyphenyl]-3,7-dihydroimidazo [1,2-a]pyrazin-3-one) and sodium azide.

The rate constants for [1O2] [MCLA] and [1O2] [NaN3] were measured by quenching the near-infrared emission (1 delta g----3 sigma g) in steady state with MCLA and NaN3, respectively. 1O2 was constantly generated by energy transfer to O2 from Ar laser-excited Rose Bengal. The Stern-Volmer plots yielded the second-order rate constants of 2.94 x 10(9) M-1 S-1 and 3.83 x 10(8) M-1 S-1 for quenching 1O2 with MCLA and NaN3 in water at pH 5.4, respectively. The 1O2 + MCLA reaction emitted light with maximum at 465 nm at pD 4.5 identical to the O(2-) + MCLA reaction.

Azides↗