Age-related changes in the subsets and functions of human T lymphocytes.
Explore the source record for details and available documents.
Adolescent↗
SEARCH · PubMed Health
Results for “T-Lymphocyte Subsets”
Explore indexed PubMed citations for clinical trials, systematic reviews and public health research. Read source abstracts and follow each citation to its original PubMed record.
Quote a phrase for an exact phrase match. Source license links do not imply unrestricted reuse.
Failure to respond to influenza vaccine in the aged: correlation with B-cell number and function.
Failure to respond to influenza vaccination correlated with a decreased number and percentage of IgD-bearing PBL and a dampened lymphocyte response to PWM in a subset of healthy aged volunteers. These subjects had normal levels of serum immunoglobulins, intact T cell function, and normal numbers of E-rosetting cells.
Age Factors↗
Changes in T-cell subsets and their clinical significance in cancer patients.
The proportion of T cells that have receptors for IgG-Fc (Fc R+ T cells), the functioning of T cell helpers in immunoglobulin production and the blastogenic response of lymphocytes to PHA and Con A were measured in cancer patients. Increases in the percentage of IgG-Fc R+ T cells were observed in most patients; these were greater in patients with advanced tumours and fell within the normal range after complete surgical removal of the tumour. The functioning of T cell helpers in immunoglobulin production and response of lymphocytes to PHA were poor in those patients in whom IgG-Fc R+ T cell percentages were increased. The lymphocytes of such patients responded well to Con A. These changes in T-cell subsets appear to be related to the presence and progress of tumours.
Carcinoma↗
Antigen-initiated B lymphocyte differentiation. XIII. Different B cell subsets give different AFC-production kinetics and respond in different functional assays.
Explore the source record for details and available documents.
Animals↗
Thymus independent anti-horse erythrocyte antibody response and suppressor T cells in the Mexican axolotl (Amphibia, Urodela, ambystoma mexicanum).
Anti-horse erythrocyte (anti-HRBC) antibody synthesis was studied in normal, early thymectomized and adult thymectomized axolotls. The kinetics of the responses were similar to those described in the same species for antibody synthesis against bacterial or viral antigens. Booster injections did not induce any characteristic anamnestic responses. Early and adult thymectomized axoltls gave in three experimental groups higher anti-HRBC responses than controls. It is concluded that HRBC acts in the axolotl as a thymus-independent antigen. The enhanced response in early as well as in adult thymectomized animals can be interpreted by the presence of a suppressor T-cell activity on anti-HRBC synthesis. These results do not exclude possible thymus-dependent responses for antibody synthesis in the axolotl, although such responses were not demonstrated in urodele. The questionable lacking of some functional T-cell subsets in urodele is discussed as a working hypothesis.
Ambystoma↗
Suppression of adult B cell differentiation in pokeweed mitogen-stimulated cultures by Fc(IgG) receptor-negative T cells from cord blood.
Unfractionated T lymphocytes from cord blood suppressed adult B cell differentiation into immunoglobulin-producing cells in pokeweed mitogen-stimulated co-culture system. Cord blood T cells were fractionated into T cells bearing Fc receptors for IgG (Tgamma cells) and T cells lacking Fc receptors for IgG(Tnon-gamma cells) by rosette formation with ox erythrocytes coated by the IgG fraction of rabbit antisera followed by Ficoll-Hypaque gradient sedimentation. T gamma cells from cord blood, even though isolated after the interaction with immune complexes, showed no suppressor activity on adult B cell differentiation, whereas Tnon-gamma cells exerted strong suppression to a similar extent to that by unfractionated cord T cells. The suppressor activity on B cell differentiation by Tnon-gamma cell as well as by unfractioned T cells from cord blood was completely abrogated by irradiation with 2000 rads. These results indicated that, contrary to suppressor function found in adult T cells, the suppressor activity in cord T cells might be exerted by a T cell subset lacking Fc receptors for IgG(Tnon-gamma cells).
Adult↗
Presence of T cell-associated surface antigens on murine NK cells.
The expression of T cell-associated surface antigens on natural killer (NK) spleen cells of C57BL/6 mice was evaluated by cytotoxic depletion experiments with alloantisera prepared against the Thy 1, Ly 1, Ly 2, Ly 5, Ly 6, and NK 1 antigens. The NK activity of these nonimmunized spleen cells for YAC-1 leukemia cells was dramatically reduced by antisera to the Ly 5 and NK 1 antigens. Variable results were obtained with anti-Ly 6 sera--certain pools of this antiserum decreased the NK activity, whereas other pools showed only negligible effects. The NK activity of the same cell suspensions was not affected by antisera to the Thy 1, Ly 1, and Ly 2 antigens. In parallel tests the T cell-associated cell surface antigens of alloimmune T killer cells were similarly evaluated by cytotoxic depletion experiments. In this case, the activity of these cells was consistently diminished by antisera to the Thy 1, Ly 2, Ly 5, and Ly 6 antigens, but not by antisera to the Ly 1 and NK 1 antigens. On this basis it was concluded that the NK cells expressed a restricted subset of T cell-associated alloantigens and therefore may have been derived from the T cell lineage of lymphocytes.
Animals↗
Further characterization of the human inducer T cell subset defined by monoclonal antibody.
Evidence is presented that the OKTA+ T cell subset in man, defined by a monoclonal hybridoma antibody, provides help for B lymphocyte differentiation in a PWM driven system. Both B cell proliferation and intracytoplasmic immunoglobulin synthesis are facilitated by OKT4+ and not by OKT4- T cells. Given earlier studies demonstrating that OKT4+ T cells were necessary for generation of T cytotoxic cells and the present study that OKT+ T cells are necessary for the differentiation of B cells, it would appear that the OKT+ population is the major human T helper (inducer) subset.
Animals↗
Human thymus cells: a study of rosette-forming capacity with unsensitized and sensitized erythrocytes of various species.
Human thymus cells from 117 donors aged 2 months to 15 years, were studied for rosette-forming capacity with unsensitized and sensitized erythrocytes of various species. Viability of the thymus cells at the completion of isolation ranged from 95 to 99%. Over 90% of the total population of thymus cells were identified as T lymphocytes by either standard Es-rosette assay utilizing a 4 degree incubation with preceding centrifugation or T--LCL assay, on the average. Gravity Es-rosette assay high affinity Es-rosette assay, stable Es-rosette assay or active Es-rosette assay for identification of subsets of T lymphocytes indicate that most of the human thymus cells were capable of forming rosettes with unsensitized sheep erythrocytes at higher degrees of temperature with or without preceding centrifugation. Approximately half of the human thymus cells were also capable of forming rosettes with unsensitized allogeneic erythrocytes. Less than 3% of the thymus cells expressed receptors for the Fc portion of IgG (T gamma suppressor cells) whereas less than 1% of thymus cells expressed receptors for the Fc portion of IgM (T mu helper cells), indicating that most of the thymus cells are non-T mu/non T gamma cells. Only 1% or less of the thymus cells were identified as B cells by the SIg assay, the Em-rosette assay or the Ia-like antigen assay. There was no significant difference of any rosette assay between males and females or among various age groups.
Adolescent↗
The collaboration of T-cell subsets in the mitogenic stimulation of purified B-cell subpopulations.
A highly purified preparation of mouse B cells showed greatly decreased incorporation of [6-3H]-thymidine when stimulated with pokeweed Pa-1 mitogen or bacterial lipopolysaccharide compared with mouse splenic lymphocytes. This decreased stimulation was restored by the addition of purified T cells, but not macrophages. Nylon-adherent T cells exerted this helper activity only toward complement receptor-positive B cells (CR+B cells). whereas the helper activity of nylon-non-adherent T cells was effective only on complement receptor-negative B cells (CR-B cells). Since the helper activity of nylon-adherent T cells was completely abolished by the treatment with anti-Ia antiserum and complement but that of nylon-non-adherent T cells was not, it was assumed that Ia+T cells were helper cells for CR+B cells and Ia-T cells helper cells for CR-B cells. Moreover, these helper activities of both T-cell subsets were mediated by soluble factors, which were effective just before the onset of DNA synthesis of the corresponding B-cell subpopulations.
Animals↗
Subsets of T lymphocytes of patients with malignancies of the urogenital tract.
Explore the source record for details and available documents.
Adult↗
Characterization of human T lymphocyte subpopulations (TG and T non G): cytologic examination and cytographic analysis.
Two T lymphocyte subpopulations were separated with an immunological method. In fact recently two T cell subsets with receptors for the Fc of IgM (TM) and of IgG (TG) respectively have been described. We have specifically separated TG and T non G lymphocytes by the rosetting technique. Cytological examination was performed on the two cell fractions: the results are in agreement with that already reported. Cytographic analysis was also carried out confirming the cytologic results. The possible practical implications of these acquisitions and those already applied are discussed.
Cell Separation↗
[Involvement of lymphocyte populations in rheumatoid synovial lesions].
T and B lymphocyte proportions have been studied in synovial fluid and peripheral blood from 70 patients with classical or definite rheumatoid arthritis (RA). The results have shown that T-cell numbers assessed by E-rosettes and anti-HTLA antiserum, B-cell numbers detected by membrane immunofluorescence and EAC-rosettes from peripheral blood were similar in RA and in a control group. Conversely, "active" E-rosettes which detected a subset of mature T cells, were decreased in blood and very increased in synovial fluid. Furthermore, the percentages of mononuclear cells bearing a Fc-receptor for IgG (EA-rosettes) were higher in synovial fluid than in RA peripheral blood. These data could be in favour of the cell-mediated immunity in rheumatoid arthritis lesions.
Arthritis, Rheumatoid↗
Markers of human T cell subsets identified by alloantisera.
Analysis by the indirect fluorescence test followed by fluorescence-activated cell sorter (FACS) analysis has shown that antisera recognizing subsets of human T lymphocytes can be produced by planned immunizations involving HLA-A and HLA-B compatible donors. The reactivity of these antisera against some individuals of a population but not others shows that they recognize a polymorphic cell surface component. The reactive subpopulation largely overlaps with the JRA+ subset, which was previously shown to possess regulatory properties in functional assays. The specificity of the antisera for a T cell subset and the unrelatedness of the anti-B cell activity of the same antisera has been confirmed by two-color fluorescence tests.
Antibody Specificity↗
Local xenogeneic graft-vs-host reaction: a practical assessment of T cell function among cancer patients.
The local xenogeneic graft-vs-host reaction (XGVHR) was used as a practical bioassay to assess T lymphocyte function and immunocompetence among cancer patients. Positive XGVHR was found in 99.5% of normal donors, 70% of cancer patients with early stage disease, and 30% of cancer patients with metastatic disease (p less than 0.001). A minimum of 4.5 x 10(6) immunocompetent T lymphocytes are necessary in order to elicit a positive XGVHR. Negative reactions among cancer patients are characterized by the lack of edema fluid accumulation and the appearance of the most basophils at the test site. This suggests that insufficient amounts of lymphokines are being released by the incompetent T lymphocytes, whereas the host is capable of mounting a rejection reaction as evidenced by the appearance of the basophils. Preliminary evidence suggests that the immunologic defect detected by the XGVHR cannot be corrected by monocyte depletion. The identification of putative suppressor T cell subsets may bear immunotherapeutic implications in the future.
Animals↗
Casein-induced experimental amyloidosis. V. The response of lymphoid organs to T and B mitogens.
Functional and morphologic studies were performed on the lymphoid organs of inbred CBA/J mice receiving chronic casein administration. In the spleen, this regimen produces marked reticuloendothelial proliferation between 8 and 16 injections (preamyloid phase) and amyloid deposition between 16 and 24 injections. No amyloid was found in the thymus, lymph nodes, and bone marrow of these animals. Phytohemagglutinin and concanavalin A lymphocyte responses as measured by 3-H-thymidine incorporation were reduced in the spleen and lymph node of preamyloid animals but demonstrated partial recovery during the amyloid phase. Phytohemagglutinin and concanavalin A stimulation of thymic cells was significantly increased during both stages of amyloid induction, although the histologic studies revealed a marked involution of the thymic cortex. Lipopolysaccharide stimulation of spleen cells was normal in preamyloid and amyloid animals whereas in lymph node and bone marrow lipopolysaccharide responses were significantly decreased. The findings suggest a selective removal of subsets of T cell populations in the spleen and thymus and migration of B cells from bone marrow to the spleen during experimental amyloidosis.
Amyloidosis↗
Immunological activities of rat lymphocytes. I. Mitogenic responses and surface markers of lymphocytes from normal, thymectomized and B rats.
Lymphocytes from the peripheral blood, thoracic duct, spleen and lymph nodes of normal, thymectomized (TX) and thymectomized lethally irradiated marrow reconstituted (TXBM or B rats) Lewis rats were studied for their ability to proliferate in vitro in the presence of Con A or PHA. At the same time the lymphoid tissues of these animals were examined for the presence of B cells or T cells by immunofluorescence staining with antiserums to rat immunoglobulins or rat brain antigens (ARBS), respectively. The specificity of ARBS fort T cells was first established in both cytotoxicity and immunofluoresecnce studies with thymocytes as well as pruified T and B cells. In various lymphoid tissues from TX and B rats, cells carrying brain antigens were found which were unable to respond to Con A and PHA. Thymectomy has a lesser effect on the response to Con A that to PHA; this was particularly true in the thoracic duct where, following thymectomy, lymphocytes continued to respond to Con A but lost their ability to respond to PHA. This suggests that the response to PHA and Con A may belong to different cells' subsets but that the ability to respond to either one of these mitogens may be impaired in cells carrying known T cell markers.
Animals↗
Ala-1: murine alloantigen of activated lymphocytes. II. T and B effector cells express ala-1.
Ala-1 (activated lymphocyte antigen-1) is a murine alloantigen expressed only on activated peripheral T and B lymphocytes. The presence or absence of Ala-1 on specific functional lymphocyte subsets was determined by treating the relevant cell population with anti-Ala-1 and complement, and assaying for residual functional activity. By this method, Ala-1 was shown to be on in vivo primed killer T cells cytotoxic for allogeneic tumor cells. It was also found on helper T cells generated in vivo to sheep red blood cells, and on IgM and IgG plaque-forming cells (PFC) to sheep red blood cells. In contrast, splenic precursors of helper cells and of IgM PFC to sheep red blood cells were completely resistant to treatment with anti-Ala-1 and complement. These findings indicate that effector cells can be distinguished from their nonactivated precursors by their expression of Ala-1.
Animals↗