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Effect of a modified toothpaste technique on approximal caries in preschool children.

A 3-year, double-blind caries trial was conducted to evaluate the caries-reducing effect of a modified technique to use toothpaste. At the outset, 369 children, 4 years of age, were randomly assigned to four groups. At the end of the study, when the children were 7 years old, 281 (76%) had completed the trial. Two of the groups (test groups, n = 131) were given the following instructions regarding 'toothpaste technique': (1) to spread the paste evenly on the teeth prior to brushing, (2) not to expectorate more than necessary during brushing, (3) to filter the remaining dentifrice foam in the dentition, together with a sip of water, by active cheek movements for 1 min before expectorating, and (4) not to carry out any further water rinsings afterwards, and not to eat or drink for 2 h after brushing. The children in the other two groups (control groups, n = 150) were not given any instruction how to use the dentifrice and how to rinse after the brushing, but were, as the children in the test groups, encouraged to use the test dentifrice and to brush their teeth twice daily. Two commercial fluoride dentifrices (A and B) were compared: one of the test groups and one of the control groups used each product. Approximal carious lesions were scored on bite-wing radiographs at baseline and at the end of the study on the distal surface of the first and on the mesial surface of the second primary molars. No difference in caries increment was found between toothpastes A and B. The children in the two test groups developed a mean of 1.14 new dfs during the 3 years compared to 1.55 in the two control groups (p < 0.05). Thus, the results indicate that the modified toothpaste technique reduced approximal caries in preschool children by an average of 26%.

Analysis of Variance↗

Plaque composition, fluoride tolerance and acid production of mutans streptococci before and after the suspension of the use of fluoride toothpastes.

Although fluoride toothpastes are widely used for caries prevention, little is known about the impact of fluoride dentifrices on plaque composition. Also the issue of adaptation of mutans streptococci to grow in vivo in a fluoride environment has received little attention. Such an adaptation may be of interest as it has been suggested that adapted mutans streptococci may show reduced glycolytic activity thereby being less cariogenic. In the present experiments the impact of the suspension of the use of fluoride toothpastes on plaque composition, fluoride tolerance and acid production of mutans streptococci was studied. Pooled plaque samples from the lingual surfaces of the lower incisors were collected from individuals (n = 13) just before and 7 weeks after they had replaced their fluoride toothpastes (0.1-0.15% F) with a non-fluoride one. The samples were analysed for fluoride and the numbers and proportions of streptococci, Streptococcus mutans, Streptococcus sobrinus, Actinomyces species, and lactobacilli, respectively. The fluoride tolerance of the mutans streptococci was estimated by culture of the plaque samples on TYCSB agar supplemented with of 0, 1, 2, 3, 4, and 5 mmol/l fluoride (NaF) at pH 7.2. From each plaque sample six S. mutans strains were isolated for the measurement of the rate of acid production (Vap) at pH 7 in the presence of 0, 5, and 10 mmol/l F. The overnight final pH was measured in cultures of the S. mutans strains with excess of glucose and 0, 5, and 10 mmol/l F. The results showed that the removal of the fluoride pressure from plaque did not affect the numbers or proportions of the various species and genera of bacteria. The fluoride tolerance of the mutans streptococci, and the Vap or the overnight final pH of the isolated strains had not changed. These results suggest that the use of fluoride toothpaste had not affected plaque composition, nor fluoride tolerance or acidogenicity of mutans streptococci. Probably the amount of fluoride delivered by fluoride dentifrices to dental plaque is too low to induce such adaptations.

Adaptation, Physiological↗

Dentin morphology and permeability after brushing with different toothpastes in the presence and absence of smear layer.

BACKGROUND: The purpose of this study was to evaluate the morphology and permeability of dentin after brushing with different toothpastes in the presence and absence of smear layers. METHODS: Dentin discs were prepared from extracted third molars. Dentin permeability was measured using a hydraulic pressure apparatus working at 70 cm H2O pressure. Dentin was treated with 0.5 M EDTA for 5 minutes and washed to remove a smear layer and to establish the maximum permeability of each dentin disc, which was expressed as 100%. A new smear layer was then created on the upper surface using a #400 carbide paper under water for 30 seconds. Dentin permeability of the smear layer-covered dentin was measured and expressed as a percentage of the maximum permeability of that specimen, permitting each specimen to serve as its own control. Each sample was then brushed by a mechanical device under water for 3 minutes with constant pressure of 250 g using a medium toothbrush and permeability remeasured. Finally, 1 of 5 different toothpastes was applied on the dentin and brushed for 3 minutes. In another group, the same procedures were performed with the exception of the smear layer production, so it was possible to calculate changes in the permeability of dentin with open tubules following brushing. Scanning electron microscopic (SEM) examination of dentin was obtained before and after treatments with brushing and toothpastes. RESULTS: Dentin permeability was reduced by brushing procedures when the smear layer was absent, but it was increased when the smear layer was present. Toothpaste application reduced dentin permeability when no smear layer was present on the top of the surface, but modified and increased permeability of samples with smear layers. SEM observations demonstrated the presence of dentifrice particles on dentin surfaces and inside dentinal tubules, and this may be responsible for the observed reductions in permeability. Smear plugs produced during dentin brushing were not removed by the dentifrices. CONCLUSIONS: Dentin permeability and morphology are significantly affected by toothbrushing and by the type of dentifrice used. The presence of smear plugs in the dentin may decrease severity.

Adult↗

Can toothpaste or a toothbrush with antibacterial tufts prevent toothbrush contamination?

BACKGROUND: Periopathogens and cariogenic species survive more than 48 hours on toothbrushes even under dry storage. This study examined a decontamination of toothbrushes by means of the bactericidal effect of different toothpastes or a special coating of the tufts. METHODS: Eight untreated periodontitis patients were professionally brushed, using a new toothbrush per quadrant and 3 different toothpastes containing either amine/stannous fluoride (AmF/SnF2), amine fluoride (AmF), or a mild surfactant as major antibacterial component (excipient), or no paste (C). The brushes were rinsed and stored dry at room temperature. At different time intervals, 4 tufts per toothbrush were processed for selective and non-selective culturing procedures. The protocol was repeated comparing AmF/SnF2 and C with 2 toothbrushes with coated tufts (coat1 and coat2). RESULTS: At baseline, C brushes (n = 16) harbored 10(7), 10(8), and 10(6) colony forming units (CFU) of aerobic, anaerobic, and black pigmented species, respectively. After 24 hours 10(6) CFU of aerobic and anaerobic species could still be cultured from 14 of the 16 brushes. Black-pigmented species remained detectable up to 4 hours; detection frequencies for Fusobacterium nucleatum and Streptococcus mutans at 24 hours were 5/16. With AmF/SnF2 and AmF toothpaste the number of adhering bacteria was already 2 log lower at baseline (P<0.05), but not for the excipient (0.5 log, P = 0.7). With AmF/SnF2 no vital species could be detected after 8 hours. AmF reduced the bacterial survival rate significantly more than the excipient, but less than AmF/SnF2 (P<0.05). The bactericidal effect of coat1 and coat2 was negligible when compared to C (P>0.20), and clearly inferior to the AmF/SnF2 paste (P<0.0001). After 24 hours these brushes still harbored >10(5) CFU. CONCLUSIONS: Toothpastes can significantly reduce contamination of toothbrushes, but their bactericidal activity is dependent on their composition. Coated tufts failed to limit the bacterial contamination.

Adult↗

Effects of a lactoperoxidase-system-containing toothpaste on dental plaque and whole saliva in vivo.

The effects of a lactoperoxidase-system-containing toothpaste. Biotene, on saliva and dental plaque were studied. In a double-blind crossover study 20 healthy volunteers used an experimental (comprising the complete peroxidase system) or a placebo (without lactoperoxidase, KSCN, and glucose oxidase) toothpaste twice daily for 2 weeks separated by a 2-week washout period. At base lines and at the end of both test periods saliva and plaque samples were collected, and plaque pH changes were monitored. Saliva was analyzed for hypothiocyanite (HOSCN/OSCN-) and thiocyanate (SCN-) concentrations and salivary peroxidase activity. The amount of total streptococci, mutans streptococci, lactobacilli, and total anaerobic flora was determined both in saliva and in plaque samples. The accumulation and the acidogenicity of plaque were also quantitated. A 2-week daily use of Biotene had no effect on salivary flow rate, peroxidase activity, HOSCN/OSCN-, SCN-, or any of the monitored bacterial counts compared with the placebo toothpaste. The accumulation of dental plaque was not affected by the lactoperoxidase-system-containing toothpaste. The acidogenicity of plaque did not change significantly, nor did the two test dentifrices differ in their ability to inhibit the plaque pH drop caused by sucrose in subjects with normal salivary flow rate.

Adolescent↗

Effects of fluoride toothpaste on etched enamel of orthodontic patients.

OBJECTIVE: To investigate the effects of fluoride toothpaste on acid-etched enamel by means of a scanning electron microscope. METHODS: Permanent first premolars extracted for orthodontic reasons were etched with 37% H3PO4. They were divided into two groups in pairs of contralateral teeth. Group 1 was treated with fluoride toothpaste slurry; Group 2 was treated with deionized water. Treatment time was three weeks (one hour, 3 times a day). RESULTS: At the conclusion of the experiment, large amounts of precipitate were observed on the enamel surface of the teeth that were treated with fluoride toothpaste slurry. The etched structures were no longer evident and the enamel surface was smooth. The acid resistance of the teeth was significantly enhanced. However, clearly etched enamel structures were visible on the enamel surface of the teeth that were treated with deionized water. CONCLUSION: Fluoride toothpaste could promote the remineralization of etched enamel and enhance its resistance to acid attack.

Acid Etching, Dental↗

A clinical study to assess the anticalculus efficacy of a new dentifrice containing a special grade of silica (Colgate Total Plus Whitening Toothpaste): a clinical trial on adults.

The objective of this double-blind clinical study, conducted using the Volpe-Manhold design for studies of dental calculus, was to confirm the supragingival anticalculus efficacy of a formulation variant of a commercially available anticalculus dentifrice. The commercially available dentifrice (Colgate Total Toothpaste) contains 0.3% triclosan and 2.0% PVM/MA copolymer in a 0.243% sodium fluoride/silica base. The new formulation variant contains those same ingredients, of which 10% of the silica is a high cleaning grade (Colgate Total Plus Whitening Toothpaste). In this study, the dentifrice formulation variant was tested for anticalculus efficacy against a negative control dentifrice containing 0.243% sodium fluoride/silica. Adult male and female subjects from the northern New Jersey area were entered into the study based on a pre-test (baseline) Volpe-Manhold Calculus Index score of 7.0 or greater, provided a full oral prophylaxis, and stratified into two treatment groups which were balanced for age, sex, and baseline calculus scores. Subjects were instructed to brush their teeth twice daily (morning and evening) for one minute with their assigned dentifrice, using a soft-bristled toothbrush. Examinations for dental calculus were again performed after eight weeks' use of the study dentifrices. Sixty-three (63) subjects complied with the protocol and completed the entire study. At the eight-week examination, subjects in the Colgate Total Plus Whitening Toothpaste group exhibited a statistically significant 34.13% reduction in mean Volpe-Manhold Calculus Index score as compared to the negative control dentifrice group. Thus, the results of this clinical study support the conclusion that a new dentifrice formulation variant containing a special grade of silica (Colgate Total Plus Whitening Toothpaste) is efficacious for the control of the formation of supragingival calculus.

Adult↗

Toothpastes.

It is well known that plaque is the main cause of caries and parodontopathies, which puts the role of toothpastes as an important means of prevention of odontopathies to the foreground. Toothpastes can be more or less oriented towards having a particular effect, such as cleaning, anticaries effect, antimicrobial effect or inhibition of the formation of tartar. Toothpastes contain substances which promote dental health, such as abrasives (silicium dioxide, brushite, calcite, calcite and aragonite, gibbsite etc.), active components (fluorides, triclosan, metal ions, sanguinarine and surface-active substances), substances which motivate the use of toothpaste (sweetening agents, aromatic oil, colours) and components which are necessary for technical reasons, such as moisturizing agents, binders and opacifiers.

Alkaloids↗

[Clinical observation of toothpaste containing zanthoxylum nitidum extract on dental plaque and gingivitis].

OBJECTIVE: To observe the clinical efficacy of drug toothpaste containing 2% Zanthoxylum Nitidum extract in reducing accumulation of dental plaque and occurrence of gingivitis in adults. METHODS: Adopting double-blind, stratified and parallel design, the status of dental plaque and gingivitis in 200 healthy adults enrolled in the trial (100 in the treated group took the drug toothpaste to clean teeth, and 100 in the control group use common toothpaste instead) were assessed at before experiment, 3 months and 6 months after experiment. RESULTS: At the end of the 3rd month and the 6th month, as compared with the control group, the L e-Silness Gingival Index (GI) in the treated group was decreased by 9.1% (P > 0.05) and 13.9 % (P< 0.5), in the same period, the Quigley-Hein(Turesky Modification) Plaque Index (PlI) decreased by 18.7% and 22.6% (P<0.05), respectively. CONCLUSION: Toothpaste containing Zanthoxylum Nitidum extract can obviously decrease the incidence of dental plaque and enhance gingival health.

Adolescent↗

Effect of amine fluoride (AmF)/stannous fluoride (SnF2) toothpaste and mouthwashes on dental plaque accumulation, gingivitis and root-surface caries.

The aim of the study was to assess the effect of a toothpaste and mouthwashes containing amine fluoride (AmF) and stannous fluoride (SnF2) on dental plaque, gingivitis and root-surface caries. Forty-four adults participated in a five-month double-blind study. The following combinations of toothpastes and mouthwashes were used: (1) AmF/SnF2 toothpaste and AmF/SnF2 (Meridol) mouthwash (20 patients, mean age 45.7 years), (2) sodium fluoride (NaF) toothpaste and NaF mouthwash (24 patients, mean age 48.8 years). The mean values for dental plaque (Silness-Löe index) were clinically and statistically significantly reduced in both groups, the difference being greater in the AmF/SnF2 group. Sulcus-bleeding index values were clinically and statistically significantly decreased in both groups. There was no marked difference between the groups. The root caries index (RCl, Katz) had decreased by the end of the experiment by 10% in the NaF group and by 47.4% in the AmF/SnF2 group.

Amines↗

Effect of anti-sensitive toothpastes on opened dentinal tubules and on two dentin-bonded resins.

Treatment of dentin hypersensitivity will become of increasing importance in the daily practice of dentistry. The goal of any therapy should be the closure of opened dentinal tubules. The present study made an SEM evaluation of the effect of anti-sensitive toothpastes on root dentin with regard to the superficial changes and possible penetration by toothpaste particles. Further, the study performed an evaluation of the wear-resistance of two dentin-bonded resins during brushing, using the same anti-sensitive toothpastes. Having induced coverage of the dentinal tubules with the toothpastes (Sensodyne, Emoform and Macleans Sensitive), no particles were found within the tubules. The application of dentin-bonded resins seems an alternative solution for treating dentin hypersensitivity.

Acrylic Resins↗

[Determination of fluorides and fluorophosphates in drugs, toothpastes and mineral waters by gas liquid chromatography].

Fluorides and monofluorophosphates present in drugs, toothpastes and mineral waters are converted into an organic compound by trimethylchlorosilane at acidic pH. The trimethylfluorosilane formed is determined with isopentane as internal standard by gas liquid chromatography with flame ionization detection. Some drugs containing fluoride at therapeutic or physiologic concentrations also some toothpastes and mineral waters were analyzed easily by this method. No interferences due to excipients or to other active substances present in drugs or toothpastes were observed with this method. Because of its sensitivity (0.01 ppm), its accuracy (CV: 0.7 à 2%) and its simplicity, the chromatographic method proposed is suitable for the routine controls of fluorides in drugs, toothpastes and mineral waters.

Chromatography, Gas↗

[Evaluation of commercially available fluoride containing toothpastes].

The role of fluorides in caries prevention has been well established in the epidemiological studies of fluoridated areas. Without water fluoridation, dentists in Taiwan face the challenge to optimize the use of other forms of fluoride products to reduce caries incidence. Toothpaste containing fluoride constitutes an effective and practical way of exposing teeth to fluoride on a regular basis. The present study evaluated 62 commercially available toothpaste products, of which 35 lacked a proper Chinese label and product information. Forty-one products contained fluorides which were sodium fluoride and/or sodium monofluorophosphate. Half of the children's toothpastes did not contain fluoride. The statement of CNS 439 only indicated that the fluoride concentration of the toothpaste was below 1000 ppm. More information about type of fluoride and its concentration must be provided for the user or dental professionals to make a proper selection. Parental supervision of their children's toothbrushing needs to be reinforced. Certain products used for dental hypersensitivity contained high concentrations of fluoride. Young children must be guarded from accidentally ingesting an overdose of fluoride.

Evaluation Studies as Topic↗

Effect of plaque of five toothpastes used in the treatment of dentin hypersensitivity.

During a study to assess the efficacy of five toothpastes in the treatment of dentin hypersensitivity, the plaque scores of patients were recorded at baseline, 2, 4, and 6 weeks treatment and 6 weeks post-treatment visits. Baseline buccal and lingual plaque indexes were found comparable between the groups and the mean buccal plaque scores were considered low by normal standards. During the use of the three experimental silica-based pastes, one containing strontium acetate, one strontium acetate and MFP and a commercially available formaldehyde-containing paste, plaque scores fell significantly and to a similar extent in all four groups. After cessation of treatment at 6 weeks, plaque scores had increased back to baseline in all groups. During the use of a diatomaceous earth/strontium chloride toothpaste, commercially available, plaque scores increased significantly, both from baseline and by comparison with the other four pastes. Nevertheless, plaque scores in this group returned to baseline levels after cessation of treatment and a return to use of a conventional non-desensitizing toothpaste. The effects of all but the diatomaceous earth/strontium chloride toothpaste would not be inconsistent with previous experience in toothbrushing studies where patients are monitored regularly. The effects on plaque did not necessarily coincide with the previously reported treatment benefit of the respective products in dentin hypersensitivity.

Adult↗

Toothpastes containing 0.3% and 0.5% triclosan. II. Effects of single brushings on salivary bacterial counts.

For antimicrobials such as chlorhexidine antiplaque activity appears due to persistence of considerable antimicrobial action in the mouth. The substantivity of antimicrobial compounds can be evaluated by measuring the magnitude and duration of salivary bacterial counts following a single exposure. Such data has been shown to correlate with plaque inhibitory properties of antimicrobial compounds. This study measured the effects of single brushings with 0.3% and 0.5% triclosan with or without PVM/MA copolymer in toothpastes on salivary bacterial counts in 14 young adults. The results were compared with single rinsing with a 0.2% chlorhexidine solution, water and a single brushing with a commercial fluoride toothpaste. The study was a 7 cell cross-over design, randomized using a 7x7 latin square design. Chlorhexidine produced large and significant reductions which persisted to the 7 hour measurement time with no indication of count recovery at this time. The triclosan pastes containing the copolymer produced significant reductions which did not persist beyond 5 hours and evidence of bacterial count recovery with a paste containing triclosan but no copolymer (increments) was apparent after 30 minutes. The water and control paste produced no significant effects on counts. Intertreatment comparisons showed the chlorhexidine rinse more effective than all other preparations in all time periods. Some of the triclosan toothpastes containing the copolymer produced significantly greater effects compared to water or the control paste at some, but not all time periods. There was little difference between the triclosan pastes with the copolymer. Nevertheless, the duration and magnitude of salivary bacterial count reductions seen with the triclosan toothpastes may explain the plaque inhibition effect.

Adult↗

Potential benefits of toothpaste advertising on dental health.

This paper considers the effect of advertising on the sales of various brands of toothpaste. It has attempted to investigate the relationship between the level of sales of a brand and those of its competitors. Econometric estimations of the demand-function for toothpaste have been made and the authors have tried to determine the interrelationships between advertisement and toothpaste sales. The findings cast doubt upon the efficiency of the present system of competitive advertisement and suggest a possible alternative approach to the marketing of toothpaste which could increase sales and, at the same time, beneficially influence dental health.

Advertising↗

[Clinical and experimental research on some toothpastes].

Authors assayed in vitro 9 toothpastes (signed A to I) against 8 types of microorganisms. Toothpaste D yielded best results, toothpastes A and B shown no antibacterial power; the other ones had intermediate degrees of effectiveness. Authors also performed an in vivo study testing 3 toothpastes on to 5 healthy volunteers, but results are not statistically significant.

Candida↗

Safety evaluation of toothpaste containing chloroform. I. Long-term studies in mice.

In three experiments, chloroform was administered to mice by gavage in a toothpaste base or in arachis oil, in doses up to 60 mg/kg/d on 6 days/wk for 8 wks. Control groups were left untreated or given vehicle only. In general, there were more survivors in chloroform-treated groups than in the controls. In the case of the males of three strains (C57BL, CBA and CF/1), treatment was associated with no adverse affect on the incidence of any type of neoplasm or any other parameter. In the males but not the females of a fourth strain (ICI) and in doses of 60 mg/kg/d but not of 17 mg/kg/d, exposure to chloroform in toothpaste base as a vehicle was associated with increased incidence of epithelial tumours of the kidney. A more pronounced effect of the same kind was seen in mice given 60 mg CHCl3/kg/d in an archis oil vehicle. This treatment was also associated with a higher incidence and severity of non-neoplastic renal disease. The mechanisms underlying the peculiar strain- and sex-specific susceptibility of ICI male mice to develop renal tumours when exposed to chloroform remain obcure; spontaneous renal tumours were also seen in vehicle control mice and possible ways in which this tendency may be enhanced by chloroform treatment are discussed. At the dose levels tested, namely 113 and 400 times average human exposure levels from the use of toothpaste (with 3.5 percent chloroform content), no adverse affect was seen in the liver and there was no increased incidence of liver tumours even in the higher liver tumour susceptible CBA strain. At the 17 mg CHCl3/kg/d level, equivalent to 113 times average human exposure from toothpaste use, no excess of renal tumours was seen even in males of the peculiarly susceptible ICI strain.

Animals↗