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PubMed1976

Bacteriophage typing of Mycobacterium tuberculosis strains isolated in South East England.

The species Mycobacterium tuberculosis may be subdivided by its susceptibility to lysis by bacteriophages. In this study 300 strains have been typed with six phages to determine the prevalence of various phage types in South East England. The distribution of the phage types is considered in relation to the patients' racial origins and the clinical presentation of disease, whether pulmonary or extrapulmonary. The technical aspects of phage typing are described and modifications of existing techniques are discussed.

Bacteriophage Typing↗
PubMed1988

Stabilities of lyophilized Staphylococcus aureus typing bacteriophages.

Staphylococcus aureus bacteriophages (25 phages) were lyophilized in aliquots 12 to 18 years ago and stored in vacuo at -20 degrees C. Eight viruses each lost one log titer, while seventeen retained the original titers. The use of lyophilized phages provided more reproducible phage typing and reduced by 75% the complexity and cost. This important test is thus made feasible for more laboratories.

Bacteriophage Typing↗
PubMed1986

Preliminary studies on bacteriophage typing of Mycobacterium tuberculosis strains isolated in Salamanca (Spain).

A phage typing of 202 strains of Mycobacterium tuberculosis isolated in Central and Northwest Spain was carried out. The commonest phage type was A (64%) and within this type A (45%). This was followed in frequency by phage type B (26%) and in last place type I (10%). No relationship was observed between the phage type and the geographical or anatomical origin of the strains.

Bacteriophage Typing↗
PubMed1977

Bacteriophage-typing designations of Salmonella typhimurium.

The phage-typing scheme of Callow (1959) has been extended. The original number of types was 34; this has now risen to 207. Tables are presented which show the provisional type designations and the definitive designations now being introduced.

Bacteriophage Typing↗
PubMedDate not supplied

[Bacteriophage typing of 823 "Listeria monocytogenes" strains isolated in France from 1958 to 1978 (author's transl)].

This study was undertaken to establish a typing scheme for Listeria monocytogenes. A total of 823 strains, isolated in France from 1958 to 1978, were studied; 69.4% of these belonged to serotype 4. Using a set of 20 phages, the lytic activity, frequency and specific character of these phages were estimated and phage typing carried out. We were able to define a phage typing system for L. monocytogenes using 12 principal phages and 3 secondary phages. In order to discriminate phages types within serotype 1, the phages 1, 3, 4, 5, 6 and 7 were used, and for phage types within serotype 4, the phages 10, 11, 14, 15, 16 and 17. Secondary phages 8, 9 and 20 had a weak lytic activity, but their specificity was very high. The lytic patterns obtained with the phages 9 and 20 were restricted to strains of serotype 5. We established a complete correlation between the lytic pattern of phages 1 to 8 and serotype 1, just as there was a correlation between the lytic pattern of phages 10 to 19 and serotype 4. Using the set of 20 phages we were able to type 645 of 823 strains of L. monocytogenes. Thus we were able to type 78.4% of all the strains examined. This percentage was very different according to the serotype of the strains tested: 88% of strains of serotype 4 and 57% of strains of serotype 1. With this set of phages 552 of 645 typable strains could be subdivided into 8 principal phagetypes: 3 types within the serotype 1 and 5 others in serotype 4. This phage typing system can be used in some epidemiological situations, in taxonomic investigations or as bacterial markers.

Animals↗