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[Electron microscopic observation of ependyma (author's transl)].

The fine structure of the normal ependymal cell has been described. The ependymal cells form a closely knit single layer lining the ventricles. They are bordered on one side by the ventricular lumen and by the neuropil on the basal surface. The luminal surface is characterized by microvilli and cilia. The former differ from those seen in the respiratory tract or intestine in that they have a smooth surface and are devoid of a coating material. They differ from the microvilli of the choroid plexus by being straight and relatively stubby. The cilia appear indistinguishable from those seen on other cells and display the usual 9+2 microtubuler structure. The apposing surfaces of adjacent ependymal cells show various junctional devices such as desmosomes and others, but apparently no true tight junction such as seen in the choroid plexus are present. The basal surface abuts directly on the neuropil usually with no intervening basement membrane. The cytoplasm contains usual organelles including fine (60-90 A) filaments and glycogen granules. The latter two structures become especially abundant under pathological conditions which elicit an ependymal response. These changes very closely resemble those seen in reactive astrocytes.

Choroid Plexus↗

[The pathomorphological characteristics of the vascular plexuses and ependyma of the cerebral ventricles in human craniocerebral trauma].

The morphology of the studied substrates was analyzed in lethal craniocerebral injuries of various origins and in various periods after it was suffered. The data on the pattern and time course of pathomorphologic shifts and functions extend the general notions on the role of the liquor circulation system in the pathogenesis of craniocerebral injury. These results may serve as additional expert criteria of the mechanisms of brain injury.

Autopsy↗

[Changes in the hypothalamus and adjacent ependyma after hormonal stimulation of the ovaries in ewes].

Superovulation treatment leaves alternations in the controlling regions of the hypothalamus and in the adjacent ependyme after ovulation. The test ewes were synchronized with Agelin (20 mg chlorsuperlutin in one vaginal sponge) and stimulated (after the removal of the sponges) with 750 IU PMSG + 750 IU HCG and with 1000 IU HCG and 750 IU PMSG + 5 ml Antisergon (goat antiserum against PMSG), administered 68 hours after PMSG (i.e. 40 hours after HCG). The control ewes were in different stages of the ovarial cycle. The experimental ewes were killed 120 to 130 hours after the start of stimulation. Routine histological techniques were used to treat the brain samples; this treatment was followed by assessment under light microscope. The ependyme epithelium of the third cerebral chamber was studied under scanning microscope. Preparations with different FSH:LH ratios had different effects on the nucleus ventromedialis. Antisergon administration influenced the secretion of NPV (prevented persistent stimulation), which was observed after administration of PMSG + HCG. On the surface of the lower part of the third cerebral chamber the administration of Antisergon slowed the formation of the miniblebs. Supraependyme cells disappeared after stimulation for superovulation.

Animals↗

Ependyma of the goat. I. The third cerebral ventricle: scanning electron microscopy study.

Ependymal surface of the III-rd cerebral ventricle of 13 goat-males and females and in the testicular hermaphrodite was studied by means of scanning electron microscope. The ependymal surface structure of infundibular recess is in relation to the sex, in case of females to the ovarian cycle phase and in males to the season. The supraependymal cells were always present with the exception of the "rest" period of males. The smallest number of the SEC was found in females during anestrus period, the highest one during proestrus and in the hermaphrodite. The supraependymal cells of goats resemble mostly the macrophages, the supraependymal neuron-like cells were present on median eminence of all animals and on rostral part of infundibulum of the hermaphrodite. The finding on the hermaphrodite's genital organs resembled the incomplete developed male without the female genital organ traces. The number, topography and external morphology of the SEC, however, were in agreement with the data of goat-females during proestrus. The results of the III-rd cerebral ventricle demonstrate the ependymal tanycytes participation in all processes occurring during the reproduction cycle of goats.

Animals↗