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At least 235 records · Page 13Linked to original sources
PubMed1983

Hormones and human milk.

The presence of several hormones in milk occurring either by passive diffusion or by active concentrating mechanism from blood can be of great concern to the neonate. According to Fulkerson [1979] when the clearance rates of the hormones out of blood is considered, this worry is unwarranted. Concerning the steroids, when their levels have been monitored in milk after lactogenesis, the worry for the baby seems to be unfounded. But with the new finding of high level of LH-RH in milk whose concentration far exceeds that of blood, care should be taken to examine in the neonates whether the levels of LH and FSH become disproportionately high and induce the secretion of gonadal steroids at a higher level.

Adrenal Cortex Hormones↗
PubMed1980

Socioeconomic status, urine estrogens, and breast cancer risk.

Urine estrogens were measured in 46 women students, ages 15-18, at a middle-class high school in Athens and in 40 women of the same age residing at one of three orphanages in the same city. The lower socioeconomic status (SES) of the latter group was documented by their lower mean height (by 5.2 cm) and weight (by 5.3 kg) relative to the high school students. Both in follicular and luteal phases of the menstrual cycle, the women with lower SES had 50% higher estriol ratios (ratio of the concentration of estriol to the sum of the concentrations of estrone and estradiol). In luteal specimens the concentration of all three major estrogens was higher in the group with low SES than in the women in the other group, but the concentration of estriol was most increased. There was also an indication of less frequent anovular cycles among the women with low SES. These findings are consistent with hypotheses linking either the estriol ratio or the frequency of anovular cycles to breast cancer risk.

Adolescent↗
PubMed1980

[Immunofluorescent analysis of intracellular localization of estrogen and progesterone in normal human endometrial cells (author's transl)].

Intracellular localization and the heat-dependent redistribution of estrogen and progesterone in human endometrial cells have been investigated by a fluorescent steroid-antibody technique. The dispersed endometrial cells were incubated with 5 X 10(-8) M estradiol-17 beta and progesterone in TC medium 199 containing 10% calf serum for 1--3 hr at 4 degrees C or 37 degrees C. An indirect immunofluorescent technique using FITC-labeled anti-rabbit IgG and steroid antibodies raised from rabbits immunizing with estradiol-6-oxime-BSA and progesterone-3-oxime-BSA was applied to the smear specimens. In normal endometrial cells in both proliferative and secretory stages, specific fluorescences to estradiol and progesterone were generally observed in the cytoplasm after incubation with the steroids at 4 degrees C for 1 hr. When these cells were incubated with the steroids at 37 degrees C for 1 hr, cytoplasmic and predominant nuclear fluorescences were detected, whereas the 3 hr-incubation at 37 degrees C resulted in disappearance of cytoplasmic fluorescence, remaining nuclear fluorescence alone. These fluorescences were remarkedly eliminated when endometrial cells were incubated with diethylstilbestrol and R-5020 prior to the incubation with estradiol and progesterone, respectively. These results indicate that the fluorescent steroid-antibody technique used in this study enables us to visualize subcellular localization of estradiol and progesterone possibly bound to receptors in each endometrial cell.

Endometrium↗
PubMed1981

Enzyme immunoassay for specific determination of the synthetic estrogen, ethynyl estradiol, in plasma.

In this enzyme immunoassay for ethynyl estradiol, a conjugate of the 3-(O-carboxymethyl)ether with horseradish peroxidase is used as the label and a conjugate of the 6-(O-carboxymethyl)oxime with bovine serum albumin as the immunogen. A solid-phase antibody procedure is used in separating antibody-bound and free steroid. The lower limit of sensitivity of the assay is 2 pg per assay tube. Interference by synthetic progestagens was minimized by extracting the sample with an anti-estrogen serum before assay. Results obtained with a comparison radioimmunoassay and this procedure agreed well (r greater than 0.98).

Contraceptives, Oral↗
PubMed1982

[Impact of synthetic sexual steroids on blood volume (author's transl)].

The isotope dilution technique was used to study the oestrogen component of "Deposiston", a long-acting contraceptive, and its effects on blood volume. Blood volume increased by about ten per cent within 15 days from administration of 3 mg of ethinyloestradiol-sulphonate (1 mg/week). Haematocrit was reduced, at the same time. This haemodynamic initial effect should be always taken into account for any assessment of biochemical parameters in response to hormonal contraception.

Adolescent↗
PubMed1977

[Studies on the excretion of estrogen in cows following oral and percutaneous (pour-on) administration of chlormadinone acetate].

The oestrogen levels were assessed in 180 urine samples taken from 20 cows. The animals had received over 15 days 40 mg one-per-cent oily solution of chloromadinone acetate. The solution had been applied orally or by pour-on methods. The curves of oestrogen excretion were identical for both methods of application, both during and after completed administration. In other words, the effect obtainable from pour-on application is just as good as that obtainable from oral administration.

Administration, Oral↗
PubMed1981

[Excretion of ethinyloestradiol sulfonate in the human milk (author's transl)].

The problem of ethinylestradiol sulphonate excretion in human milk was qualitatively and quantitatively studied. In two women 0.0012 or 0.03000 per cent of total radioactivity were detected within the first nine days from application. Parallel thin-layer chromatography revealed the occurrence of ethinyloestradiol sulphonate in both its depot form and as ethinylestradiol.

Chromatography, Thin Layer↗
PubMed1977

Inhibitory effects of sexual steroidal hormones on the responses of the isolated guinea-pig lleum to acetylcholine and histamine.

Six sexual steroidal hormones (progesterone, pregnenolone, testosterone, ethinyl-oestradiol, oestriol and oestrone) inhibit acetylcholine- and histamine-induced contractions of the isolated guinea-pig ileum. Different degrees of inhibitory capacity were found. High concentrations of PGE1 and F2alpha reverse some of these inhibitions. This reversal seems to be non-specific and probably related to sensitization of the ileal smooth muscle by prostaglandins. The inhibitory action of sexual steroids might be non-specific as well. But a "corticoid-like" effect cannot be excluded.

Acetylcholine↗