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At least 235 records · Page 13Linked to original sources
PubMed1994

[A study on distribution of Porphyromonas gingivalis in oral cavity by using a non-radioactive DNA probe method].

The purpose of this study was to evaluate the distribution of Porphyromonas gingivalis (P. gingivalis) in the dentition using a non-radioactive DNA probe and to compare the presence of P. gingivalis with the clinical parameters. Fifteen adult periodontitis patients and 6 clinically healthy volunteers were examined. Subgingival plaque samples were taken from 4 sites of all the remaining teeth. Probing depth and bleeding on probing (BOP) were also recorded. Plaque samples were investigated using a whole genomic DNA probe from P. gingivalis modified with bisulfite. In the patient group, the detection percentage and the amounts of P. gingivalis present were statistically compared with probing depth and BOP. As a result, P. gingivalis was detected in all 15 patients and 3 out of 6 healthy individuals. The detection average was 31% in the patient group. When the probing depth was over 4mm, 6mm or the BOP was positive, the detection percentage of P. gingivalis significantly increased in the patient group. As more P. gingivalis was identified, the percentage of the sites with deep probing depth or that were BOP-positive increased. However, P. gingivalis was also detected in the clinically healthy sites, and also it was not detected in some sites with deep probing depth or that were BOP-positive. These results indicate that P. gingivalis plays an important role, but is not the only microorganism responsible for adult periodontitis.

Adult↗
PubMed2004

Oral flora in the age of molecular biology.

The present work describes the fundamental aspects of the molecular methods that are applied to oral microbiology: probes, PCR, multiple real time PCR, 16S rDNA, and proteomics. Likewise, it presents the results obtained by applying these methods to the study of the bacterial flora encountered inside the mouth. By identifying 16S rDNA, up to 132 known species and 215 new, unknown phylotypes have been detected inside patients s periodontal pockets. We are currently using a commercial system based on multiple PCR (genomic amplification), followed by reverse hybridization to detect the five species known to cause periodontal disease. We also summarize the findings derived from the application of proteomic techniques to the study of odontologic infections pathogenesis and from the use of molecular methods in the study of resistance to antimicrobial agents. Finally, it puts forth the problems that remain unsolved with respect to oral flora and the treatment of odontogenic infections. Traditional culture methods continue to be indispensable, as they make it possible to later work with the cultured microorganisms.

Aggregatibacter actinomycetemcomitans↗
PubMed1992

Mutans streptococci in margins of fillings and crowns.

A significant correlation was found between the number of mutans streptococci in saliva and the proportional distribution of these microorganisms in pooled plaque from the margins of all restorations. At high salivary numbers there was a tendency towards a higher porportion of mutans streptococci in plaque from the margins of separate restorations. At low and medium salivary numbers, plaque samples could, however, contain greater than 10 per cent mutans streptococci and even less than 1 per cent was found in plaque from margins of restorations at high salivary numbers. The reproducibility of the plaque sampling method was good. Plaque material collected on different occasions from the same margins did not vary significantly in terms of proportions of mutans streptococci. The numbers of mutants streptococci in plaque samples from different restorations of the same kind collected on the same occasion in the same mouth could, however, show a considerable variation. The observations made in this study, indicate that a low salivary number of mutans streptococci does not exclude a high proportion of these microorganisms in plaque from the margins of separate restorations. This has to be considered when efforts are made to reduce the risk of recurrent decay.

Adult↗
PubMed1995

Eliminating effects of an air purifier on infectants during dental procedure.

Blood and drill dust from dental plaque microorganisms, teeth, and filling materials can cause environmental pollution in the dental clinic. Currently, as a preventive measure against air pollution from a patient's mouth during dental treatment, dust-collecting aspirators such as an extra-oral vacuum aspirator (EOVA) are coming into general use. We tested the eliminating effects by the EOVA with the plaque solution aerosol and the aerosol from drilling a tooth by examining the distribution of floating aerosol in the air turbine's tank when a plaque solution was sprayed and when a human tooth was drilled with a plaque solution. We concluded that infectious aerosol increases in diameter with the drilling of human teeth to the size of about 0.5-5.0 micrometers, which is microbiologically and hygienically hazardous and also can be inhaled without much difficulty.

Aerosols↗
PubMed2002

[Occurrence of bacteria in the mouth from genera of Micrococcus, Kocuria, Nesterenkonia, Kytococcus and Dermacoccus].

The aim of the study was to assess the prevalence of different bacteria in the oral cavity. The bacteria were present in the oral cavities of 73 (48.7%) of 150 individuals. Nesterenkonia halobia, the most frequently isolated species, was found in 20 (27%) individuals, Micrococcus luteus in 16 (22%), Kocuria kristinae in 12 (16%), Kocuria varians in 10 (14%), Dermacoccus sedentarius in 9 (12%), Micrococcus lylae in 8 (11%), and Kytococcus nishinomiyaensis in 3 (4%). Mean counts of these microorganisms were relatively low and amounted in log10 CFU/ml saliva for M. luteus 1.87 +/- 0.52, for M. lylae 2.03 +/- 0.39, for N. halobia 2.14 +/- 0.56, for K. kristinae 2.20 +/- 0.69, for K. varians 2.19 +/- 0.67, for K. nishinomiyaensis 1.72 +/- 0.39, and for D. sedentarius 2.27 +/- 0.55. The factor limiting the population sizes of these microorganisms was most probably the antagonistic activity of the bacteria living in oral cavity.

Adolescent↗