Two-way avoidance learning in pigeons after olfactory nerve section.
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Bilaterally nerve-sectioned male albino mice were given access to a novel food (almond) and then body rotated for 50 min. Two days later the mice were given a second access to almond. The procedures were repeated after recovery of olfactory function. Mice formed a presumably taste-mediated aversion while anosmic and a second flavor-mediated aversion (to the same food) after the return of olfactory function. Rotation control subjects formed only the standard flavor aversion subsequent to the first pairing of novel food and motion. It appears that olfaction is required for recognition of a food as novel.
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It was shown that summation action potential (AP) in various points of the nerve result from summation AP in various bundles of fibres. The rate of propagation of AP in various bundles may differ rather significantly. Propagation of AP from the site of stimulation takes place with acceleration, which is presumably due to the inhibitory effect of the stimulus at the site of its application (together with the excitatory effect as well). Both nerves exhibit also supernormal phenomenon which reveals itself in the increase of the propagation rate of the second AP during stimulation by paired stimuli.
Cytophysiology of gonadotropin-releasing-hormone neurons in chum salmon (Oncorhynchus keta) was examined before and after upstream migration by an immunocytochemical technique with a specific antiserum to salmon gonadotropin-releasing hormone and an in situ hybridization technique with an oligonucleotide encoding salmon gonadotropin-releasing hormone precursor (pro-salmon gonadotropin- releasing hormone). In the forebrain (olfactory nerve, olfactory bulb, telencephalon, and preoptic area), salmon gonadotropin-releasing hormone-immunoreactive neurons and neurons showing signals for pro-salmon gonadotropin-releasing hormone mRNA were compared between fish from the coastal sea and those from the spawning ground. Neurons in the dorsal region of the olfactory nerve and in the ventral region of the transitional area between olfactory nerve and olfactory bulb showed strong salmon gonadotropin-releasing-hormone immunoreactivity and strong hybridization signals in fish from the coastal sea, but these activities and signals were not observed or were decreased in number in fish from the spawning ground. The neurons in the olfactory bulb, telencephalon, and preoptic area consistently revealed salmon gonadotropin-releasing-hormone immunoreactivity and hybridization signals, and the hybridization signals of salmon gonadotropin-releasing hormone in the telencephalon and the preoptic area were stronger in fish from the spawning ground than in those from the coastal sea. These findings suggest that salmon gonadotropin-releasing-hormone neurons in the olfactory nerve and the transitional area between olfactory nerve and olfactory bulb have different patterns of hormone production than those in the telencephalon and the preoptic area.